We narrowed to 1,010 results for: RING;
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TypeBlog Post...approach that problem in a novel way. Finally, engineering new applications to solve problems will provide...’s a good idea to move around to different labs during your career; you learn new techniques and interact...get feedback from the funding body through their scoring system or meeting with their grant submission panel...
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Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...create a successful science blog 5) Get creative – Bring your knacks into play Perhaps writing just isn’t...projects, you can set up a citizen science project. Answering the big science questions around climate change...guest blogger, Sarah Schmidt! Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas... -
Design Tips for Prime Editing
TypeBlog Post...extension begins with a C base can cause unwanted base pairing (left), while pegRNAs that use other bases in this...the cell to use the edited strand as a template during DNA repair. Test multiple nick sites, starting ... linkers that minimize unwanted intra-RNA base pairing with the primer binding site (Nelson et al., 2022... -
Engaging with science and society at pgEd
TypeBlog Post...disparities. But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists...ways of collaborating, and to organize events that bring different voices together to talk about genetics...running these events will be important both in preparing you for future work in these areas, and in helping... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...produced when the candidate proteins’ interaction brings the pieces of the FP back together (Figure 1). ...interaction in vivo or in vitro, and can be used for measuring protein expression or localization in cells, or...yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Lu et al. Plant J. 2010 Yuhai Cui... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...University. Some enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or... 2018). This means that off-target loci – in neighboring chromatin or throughout the genome – could be...that site. So, when DNMT1 methylates the genome during DNA replication, it doesn’t methylate sites blocked... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...simplicity of gene activation: rather than requiring protein engineering for each loci, CRISPR/Cas systems only...regulation. This can be used to activate transcription during either initiation or elongation, depending on which... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...publications in the fields of research they support. Monitoring these types of accounts can help you find papers...papers later. Pro tip Learn more about authors by exploring author pages (for example, Melina Fan’s author... Instead, you create your own research feed by entering keywords, concepts, journals, and/or papers of... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...faster in the following test and skew results measuring locomotion. Mice may need to be given some time...optional handling animals Experiment Behavioral scoring systems (experimenter differences in behavior observation...of our viral vectors blog posts Find tips for preparing AAVs View our Beginner's Guide to Viral Vectors... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...the moderator should be taking good notes and discovering interesting stories, riffs or opinions that each... a fun discussion. People are much better at remembering these things on a relaxed phone call than when...The idea is to ask questions in a directed way to bring out these interesting stories and remind the panelists...