We narrowed to 872 results for: cel

...experiments possible in a wide range of organisms and cell lines. In C. elegans, one of the model organisms... efficiency. Finally, as shown in Drosophila S2 cells (Böttcher et al., 2014), inactivation of non-homologous... community. Developments in mammalian and yeast cells using modified Cas9 proteins to regulate gene expression...homologous recombination donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org...

...of high profile people denying science, from celebrities falsely claiming vaccines cause autism to politicians...worried the march would be a simple, unquestioning celebration of science, at a time when scientists must rise...fellow at Vanderbilt University. She studies beta-cell function and failure in Type 2 Diabetes. Birmingham...BC (Canada). She works in a lab that uses yeast cells to study how DNA damage promotes cancer. She is ...

...ImagingResearchers: Andrea Radtke, Ziv YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos...officially the start of Addgene's winter holiday celebrations! We're kicking it off by sharing all the incredible...

...Guide RNA and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Osuka, S., Isomura...efficiency and are important for robust activity in cells (Jiang & Doudna, 2017). Within an interior cavity...it, so many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg...

... Immune cells Flow cytometry (potentially others) Data repository From the Human Cell Differentiation...repository   HuBMAP Consortium Healthy human cells Imaging (IHC, ICC, IF, etc.)  Data repository...

...nuclease target multiple genes in a cell population. The resulting mutant cells are screened for phenotypes of...range. CRISPR can be difficult to deliver. Not all cells efficiently take up plasmids used to produce CRISPR...

... ranging from new tools you can use to monitor cellular perturbations to how CRISPR has made it much easier...2016 Click on the links below to read all of our excellent posts from July: Addgene at the ASM Microbe 2016...Studies Gendered Innovations: Why Does Sex of the Cell Matter? AAV: A Versatile Viral Tool for Gene Expression...

...chimeric cellulases that enable E. coli and Citrobacter freundii to generate sugars from cellulosic biomass...inspire new Ghanaian scientists to continue to do excellent work in the biological sciences. Future awardees...

...more about. Our post on “Making Your Own Competent Cells” has been extremely popular, generating many additional... We’re happy to see scientists making their own cells to save money and create the tools that work best...Save Time and Money by Making Your Own Competent Cells By Melina Fan Plasmids 101: The Promoter Region...

...Multiplex genome engineering demonstrated in human cells They used the Golden Gate cloning method to ligate...successfully to target multiple sites in cultured human cells. While it has not yet been demonstrated, they say...Reference: Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sakuma...