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We narrowed to 954 results for: COMP

Showing: 631 - 640 of 954 results
  1. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...itself. Among other things, science communication comprises broadcasting, science writing, and certain aspects... get on stage for a Science Slam – a sort of competitive TED talk – to win the audience’s applause with...photography and participate in a science photo competition. Or nominate yourself as an educator for a short...
  2. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...structure of the hairpin-DNA complex is thought to destabilize the transcription complex, initiating cleavage ...mechanism, whereas Polymerase II termination is more complex and involves two RNA polymerase-associated proteins...
  3. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...who are usually antagonistic and impolite, Complainers, who spend most of their time moaning about their...with negative comments (hostile-aggressives, complainers, know-it all experts, wet-blanket negativists...support from your colleagues or other supervisors to complete your work. Whether your supervisor is a negative...
  4. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... strategy uses new combinations of PE protein components (Figure 1), an altered epegRNA delivered with...protein-coupled receptors (GPCRs) are critical components of many physiological pathways and represent ...et al., 2024). Each plasmid expresses all the components of the biosensor in optimal proportions from ...
  5. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...screens have gotten larger, easier, and more comprehensive thanks to the consistent development and improvement...vectors is how the cargo — in this case, the CRISPR components — are expressed. Unlike lentivirus, cargo delivered... away from the comfort of Cas9, and opt for a completely different Cas effector, like Cas12a (Cpf1). Even...
  6. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...RNA (pegRNA). The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension...accounting for some of the inherent inaccuracies of computational prediction models. In addition, we provide options...Switzerland), where he developed a strong passion for computational biology. His expertise includes applying machine...
  7. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... fluorescent signal throughout the reaction is compared to a standard curve of amplified DNA of a known...The cycle in which the unknown DNA is detected compared to the standard curve can be used to determine... to note however, that multiplex PCR is more complicated to develop and is often less sensitive than PCRs...
  8. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...proteins on the cell surface. Figure 1: Comparison of common antibody, HCab, and nanobody. For more...starting library size (up to 1012 members) in comparison to other display systems using phage and yeast...the end of the mRNA molecule, forming a ribosome complex consisting of the translated protein (sybody), ...
  9. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...the cell lysis process. To ensure that lysis is complete, use a reagent like Qiagen’s LyseBlue, which uses... uses color to show you if lysis is incomplete. Protein expression strains are not good for cloning Bacterial...the material used for inoculation. To further complicate matters, plasmid copy numbers are often ambiguous...
  10. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...service free of charge to scientists by allowing companies to pay publish their catalogs on the site. Like...of checking off each step as you painstakingly complete your experiment – one small victory in a long ...manage scientific data: Electronic lab notebooks compared with Google Docs and wikis - by Rory Macneil on...
Showing: 631 - 640 of 954 results