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We narrowed to 1,010 results for: RING;

Showing: 631 - 640 of 1010 results

  1. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... It may be helpful to incorporate regular feeds during this incubation time to maintain cell viability...consider increasing the number of washes performed during your harvest. Validation Recombinant antibodies...temperature shifts. Of course, we are also cautious during the aliquoting and storage process to prevent dropped...

  2. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...for distribution. If your experiment involves delivering a calcium biosensor using AAV, you might decide...viral preps instead of plasmids? The available filtering options now change dynamically based on the type...target organism. Clicking through to RNA Editing brings us to a page with a basic overview and figure, ...

  3. An Introduction to Adenovirus

    Type
    Blog Post
    ...inhibition of the innate immune system; and reconfiguring of the nuclear compartment, gene expression,...’ functions are rescued by helper plasmids used during production and/or by genes present in the HEK293T...license.   The promise of adenoviral vectors This brings us to the final topic, which is that natural AdVs...

  4. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ... the genome, they could promote oncogenesis by altering local gene expression. Self-inactivating (SIN)... PubMed Central PMCID: PMC4486245. 5. Patient monitoring and follow-up in lentiviral clinical trials. ... Mary Gearing...

  5. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...up colony PCR reactions is nearly identical to preparing a standard PCR reaction: combine template, primers...other colony PCR tips are highlighted below. Preparing template: Pick a single colony with a sterile ...to the PCR reaction. The bacteria will be lysed during the initial heating step of the PCR reaction. A...

  6. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...requirements. Cpf1’s preferred PAM is 5’-TTN, differing from that of Cas9 (3’-NGG) in both genomic location...repair templates should remove the PAM sequence. Comparing Cpf1 and Cas9 on-target and off-target efficiency... Mary Gearing...

  7. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ... training, mentoring and coaching services, including Smartegrants, to help those entering the Grants ...Twitter Chat, and then our grant training and mentoring. How do I become a grant professional? Employment...

  8. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...success depends directly on the choices you make during the early stages of the process. Some questions...which acceptor vectors and parts you will be using during the different levels of your MoClo assembly. Know...fragment can be used directly in your assemblies. Preparing reagents Keep it cool You’ve done all the learning...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal circuits. Epitope ...tags, low FP expression may be insufficient for monitoring a weakly expressed POI. Spaghetti Monster - A...fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 (2009): pdb-top63. PubMed...

  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1), a nuclease that generates...comment below. Note: Jennifer Tsang and Mary Gearing contributed to the update of this post. References... genome engineering...
Showing: 631 - 640 of 1010 results