We narrowed to 954 results for: COMP

...also is also called Bimolecular Fluorescence Complementation (BiFC). 8 points to keep in mind when choosing...divided by 1000. In many cases the brightness is compared to that of EGFP which is set as 1. Some proteins... BD bioscience. ilovegfp – a site with very comprehensive data sheets on many FP variants Plasmids 101...

...No examination of the state of science would be complete without the inclusion of some interesting case...Prashers will find it increasingly difficult to compete (for funding, etc.) with that produced by the confident...the U.S. environment, and a decline in the competitiveness of U.S. science and technology.” This last ...

...your samples, the relationships between cellular components, and the accessibility of your target epitope...work by dehydrating and precipitating cellular components. This method of fixation is good for epitopes...away your target (for example, some cytoskeletal components) and you want to fix and permeabilize in one ...

...engineered DNA-binding molecule such as the CRISPR complex is expressed in cells to be analyzed, the Fujii...another "in vitro" form. In this form, the CRISPR complex, consisting of a recombinant dCas9 protein and ... a genomic locus through a specific gRNA-dCas9 complex. When combined with next-generation sequencing,...

...cells can decrease the number of fluorophores, compromising the photon budget. Likewise, the switch from...labeling chemistry easier and more functional in complex biological environments such as live cells and ...brightness of the resulting conjugate, and the complexity of the system. A particularly desirable property...

...importance of the entire GFP protein, which is almost completely required to maintain fluorescent activity; very...may not apply. Table 1: The specific mutations comprising common fluorophores Fluorescent Protein Mutations...chromophore to form with an indole rather than phenol component (cyan derivatives) Y66H Blue-shifts the wavelength...

...both Addgene’s founding and our growth into the company we are today. As part of our 20th anniversary celebration...pages allowed researchers to easily access and compare their options for tools such as empty backbones...Addgene YouTube channel to add a strong visual component to our educational resources. From 2013–2023, ...

...decreased p-values of known ricin regulation genes compared to results generated using non-targeting guides...full-length (19-20 bp) or short (17-18 bp) guides. When comparing the toxicity of guides that have 1-bp mismatch...off-target sites, short guides had reduced toxicity compared to full-length guides. Reduced toxicity of short...

...laser cleavage and an RNA aptamer that bound the compound malachite green (MG) to create a non-protein based... 4-hydroxybenzylidene imidazolinone (HBI)-like compound allowing it to fluoresce, the Jaffrey lab asked...production have also made aptamers an important component of high throughput assays with microarrays and...

... amplify well and others don’t. The size and composition of your library can drastically impact what conditions...suffice…and the answer is no. Due to the size and complexity of pooled libraries, NGS is the best way to validate... plasmids didn’t amplify after your screen is completed!  Producing Your Lentiviral Library Once you've...