...Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following a cut... accessibility due to chromatin status). No computational prediction is ever perfect, but this can decrease...to conduct genetic screens, however, a library composed of the latter would likely be more effective, ...

... a post-program self-assessment, participants completed follow-up self assessments and program evaluations...knowledge and skills had improved significantly when compared to their pre-program self-assesment. Achievements...other countries. In 2016, we expect the program to comprise over 2000 participants (mentors and mentees). ...

...that you have is the expected size, or that it is composed of a backbone and insert of expected sizes. This...

...positions he has held in his career, his favorite company tradition, and Addgene's role in the scientific...

...validation is so important. Antibodies are a critical component of the researcher toolkit due to their ability...will bind to - particularly when you start using complex samples such as whole cell lysate or tissue sections...BioRender.com.     Orthogonal Strategies This approach compares target expression of antibody-independent approaches...

...In 1976, the world’s first genetic engineering company, Genentech, was founded.  This commercialization...instead of recognizing nucleotide triplets, the components recognize individual nucleotides. This imparts...intracellular pangenesis to the rediscovery of Mendel. Comptes Rendus de l’Académie des Sciences - Series III ...

...expression, and is flanked (“floxed”) by two sets of incompatible recognition sites (e.g., loxP and lox511) in... Han, 2016). Not so long ago, however, it was completely unclear which subpopulation of DA neurons could... "Two possible roles of bacteriorhodopsin; a comparative study of strains of Halobacterium halobium differing...

...transfer expert who understands the somewhat complicated Material Transfer Agreement process that kits...function. Project team A Project Team is assembled to complete a series of tasks with precisely defined goals...organization on the planet, in which case whoa! Mission accomplished! One example of a mission-based team in academia...

... However, many scientists operate in a hyper-competitive environment where getting hired hinges on performing...research biologist by training, so of course I felt compelled to participate in one of the satellite Marches...funding, and teaching experience that it takes to complete your resume, without ever thinking that research...

...attached fluorophore. However, since tRNA suppression competes with natural chain termination at stop codons,...contrast can be improved using disulfide-containing compounds such as British anti-Lewisite (which was originally...Vilensky & Redman, 2003]). Most importantly, these compounds cross cell membranes, thus enabling FRET measurements...