We narrowed to 954 results for: COMP

...decreased p-values of known ricin regulation genes compared to results generated using non-targeting guides...full-length (19-20 bp) or short (17-18 bp) guides. When comparing the toxicity of guides that have 1-bp mismatch...off-target sites, short guides had reduced toxicity compared to full-length guides. Reduced toxicity of short...

...laser cleavage and an RNA aptamer that bound the compound malachite green (MG) to create a non-protein based... 4-hydroxybenzylidene imidazolinone (HBI)-like compound allowing it to fluoresce, the Jaffrey lab asked...production have also made aptamers an important component of high throughput assays with microarrays and...

...are differentially inactivated by nonspecific complement mechanisms in serum. Sometimes this is due to...sugars end up in the envelope and are targets for complement-based killing by anti-alphaGal antibodies. The... as described above and is inactivated by the complement system in serum (DePolo et al., 2000). Other ...

...C) installation by base editors. Pathways that compete with the desired outcome are not pictured. ...Plant base editor platforms and their molecular components. Adenine base editors for plant We developed...editors. Plant CBE and ABE with expanded PAM compatibility All of the base editors mentioned above are...

...ordering the sgRNA is simple and easy. Several companies supply gRNA sequences, such as GenScript, ThermoFisher...order them yourself through whatever DNA supply company you currently use. You can also find many previously...explored and it allowed control over each individual component of the system. However, it is easier to directly...

...line identity and purity making it easier for comparable studies to be performed on a cell line even decades...passage number, cell line, and date, which you complete each time you passage the cells (as well as recording...through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune, and Infors...

...needs a standard against which the results can be compared; results from an experiment lacking the proper...baseline/standard against which other samples can be compared. The Empty Vector Control (mentioned above) could... where expression of that gene is knocked out completely. These controls may or may not be plasmid-based...

...promoter present in your plasmid backbone must to be compatible with the type of RNA that needs to be made: if...speaking, promoters in bacteria are less diverse and complex, having fewer parts than those in eukaryotic cells... repression in the presence of glucose or by competitive binding of the anti-inducer fucose Weaker. Commonly...

...recombination intermediates can then resolve to complete the DNA repair process Homology directed repair...is elongated and ligated to fill the gap, thus completing SDSA.   Break-induced repair (BIR) pathway BIR... uses reverse transcriptase to generate the complementary ssDNA. Easi-CRISPR was shown to increasing editing...

...then following transduction, the partial genomes complement each other to restore the full-expression cassette... has equal or higher transgene expression when compared to using a single over-sized vector, but not always... one of the transgene’s endogenous introns or comprised of non-endogenous sequences, such as a 77bp long...