We narrowed to 877 results for: cel
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TypeBlog Post...Voigt CA, Ellington AD. Cell. 2009 Jun 26;137(7):1272-81. doi: 10.1016/j.cell.2009.04.048. PubMed. Find...with sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so ...
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Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...to supplement your cells with biliverdin for maximal brightness. In mammalian cells, one of the improved...Imaging Center at UCSF. A common requirement for live cell imaging experiments is the ability to follow multiple... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post...bacteria, so it can be transformed as-is into cells. The cells then get to work fixing up the “damage” and...nicked plasmid together long enough to get it into cells and let them do the repair work. The key is T4 DNA... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post...20, 2021. By Will Arnold and Shreya Vedantam Accelerating research by improving the availability of materials...plasmids to help academic and industrial labs accelerate research and to help find a faster solution to...modification may not be easily expressed in non-mammalian cells. Yeast expression plasmids The yeast expression ... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...they either trace downstream from the soma (neuron cell body) toward the axon (anterograde tracer) or upstream...particles require receptor-mediated entry into the cell. This means that not all neurons and pathways will...chemogenetic vector directly into the region where the cell bodies of the projection neurons reside. Impact... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...quantitatively evaluating antibody staining, or counting cells are now routine, open-source software projects that...portable device for studying plankton using flow cells, has become an established alternative to high-priced... and blueprints has the potential not only to accelerate scientific progress, but also to enable science... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These cells can be...Enzymes Required No No Yes Yes Lab Made Competent Cells Required Yes No No No Comparison of REPLACR-mutagenesis... -
Summer SciComm: Preprints
TypeBlog Post...from peer-reviewed publishing in a journal. Accelerating scientific communication Preprints have been...Figure 1: Stand aside, CERN — it's a new age of accelerating science! Photo courtesy of Henry Mühlpfordt,...Lacy was also employed by the American Society for Cell Biology in a position working with preprints in ... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...Professional Career Coaching can be an excellent tactic for scientists making a career shift or who wish...very popular speaker and workshop leader. Lauren Celano of Propel Careers is not a professional coach but...Consulting is part of that group and she is especially excellent in coaching for communication skills. Industry... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...encode proteins. They play important roles in many cellular and regulatory processes, but much of their functionality...screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol...non-coding RNAs with the CRISPR/Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi...