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We narrowed to 872 results for: cel

Showing: 651 - 660 of 872 results
  1. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    Blog Post
    ...electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These cells can be...Enzymes Required No No Yes Yes Lab Made Competent Cells Required Yes No No No Comparison of REPLACR-mutagenesis...
  2. Summer SciComm: Preprints

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    Blog Post
    ...from peer-reviewed publishing in a journal.  Accelerating scientific communication Preprints have been...Figure 1: Stand aside, CERN — it's a new age of accelerating science! Photo courtesy of Henry Mühlpfordt,...Lacy was also employed by the American Society for Cell Biology in a position working with preprints in ...
  3. Career Coaching for Scientists: Why and Where Do I Find One?

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    Blog Post
    ...Professional Career Coaching can be an excellent tactic for scientists making a career shift or who wish...very popular speaker and workshop leader. Lauren Celano of Propel Careers is not a professional coach but...Consulting is part of that group and she is especially excellent in coaching for communication skills. Industry...
  4. Open Resources and Plasmid Tools For Studying C. elegans

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    Blog Post
    ...currently doing this using two approaches: single-cell RNA-sequencing to identify neuronal class and deep...WDDD aims to acquire quantitative data on all the cell divisions of the C. elegans embryo when all essential...Wang et al., 2018).” With the split system, only cells that have both halves of cGAL can drive expression...
  5. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post
    ...encode proteins. They play important roles in many cellular and regulatory processes, but much of their functionality...screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol...non-coding RNAs with the CRISPR/Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi...
  6. Making CRISPR Plasmids Using Fragmid

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    Blog Post
    ...Drosophilia Plasmid pRDB_051 (for stable fly cell lines; attL and attR sequences for genome integration...Drosophilia Plasmid pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration...rapid assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519. https://doi.org/10.1016/...
  7. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post
    ...triple colocalization is possible even when staining cells with three IgG1 antibodies. Antibodies were individually...workflow yields similar localization patterns as cells stained for one target (compare nanobodies staining...–rabbit IgG secondary nanobodies. The Journal of Cell Biology. PubMed PMID: 29263082 Find plasmids from...
  8. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post
    ... smaller cargos, and the ability to easily cure cells of the plasmid after integration. Figure ...into the mini-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically...distinct biological elements, CRISPR-transposons excel at programmable insertions of even very large genetic...
Showing: 651 - 660 of 872 results