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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 877 results for: cel

Showing: 661 - 670 of 877 results

  1. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...Drosophilia Plasmid pRDB_051 (for stable fly cell lines; attL and attR sequences for genome integration...Drosophilia Plasmid pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration...rapid assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519. https://doi.org/10.1016/...

  2. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...currently doing this using two approaches: single-cell RNA-sequencing to identify neuronal class and deep...WDDD aims to acquire quantitative data on all the cell divisions of the C. elegans embryo when all essential...Wang et al., 2018).” With the split system, only cells that have both halves of cGAL can drive expression...

  5. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...identifying new genes required for stem and progenitor cell maintenance in planarians in Developmental Biology... his teaching style, with the former offering excellent training in improvisation and the latter making...junction complex is required for stem and progenitor cell maintenance in planarians. Developmental Biology...

  6. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...triple colocalization is possible even when staining cells with three IgG1 antibodies. Antibodies were individually...workflow yields similar localization patterns as cells stained for one target (compare nanobodies staining...–rabbit IgG secondary nanobodies. The Journal of Cell Biology. PubMed PMID: 29263082 Find plasmids from...

  7. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ... smaller cargos, and the ability to easily cure cells of the plasmid after integration. Figure ...into the mini-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically...distinct biological elements, CRISPR-transposons excel at programmable insertions of even very large genetic...

  8. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...useful addition to the toolkit for visualizing cellular processes in C. elegans: mScarlet3 for applications...biologically relevant forces at focal adhesions in living cells in several different contexts.   Figure ...substrate furimazine. Right: Demonstration of PILATeS. Cell adhesion to a PILATeS-functionalized coverslip creates...

  9. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...As part of Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our... be safely used to deliver a genetic payload to cells, and the complexity of effectively deploying them... can be to use. AAVs, which can target different cell types depending on their serotype, are particularly...

  10. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...of experience in [field of research], and have excellent [list the most important skills for the position...your specific skills and experience will help you excel in the role. It can also convey your excitement ... specific examples. Instead of saying “I have excellent communication skills” try giving an example, like...
Showing: 661 - 670 of 877 results