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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 877 results for: cel

Showing: 671 - 680 of 877 results

  1. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...delivered as a pooled library via a lentiviral vector to cell lines that either stably expressed Cas9 or that ...almost all genes involved in the production of a cell surface glycan that’s required for ricin uptake....reveals pathways underlying ricin susceptibility." Cell 152.4 (2013): 909-922. Pubmed PMID: 23394947. Pubmed...

  2. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...score (KO-score) which represents the proportion of cells that have either a frameshift or 21+ bp indel. This...Following delivery of CRISPR components into target cells, genomic DNA from both edited and unedited (control... score. KO Score - Represents the proportion of cells that have either a frameshift or 21+ bp indel. This...

  3. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...example, "while it is possible to grow liver stem cells and liver-like mini-organs or organoids, neither...combine bioengineered scaffolds with patient stem cells to generate compatible functional livers.” Why are... optimization of fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance...

  4. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... . Steps of an IP: 1) Protein extracts from cells or tissues contain a complex mix of proteins seen...common immobilization methods utilizes bacterial cell wall proteins, Protein A and Protein G, which bind...does not. Common negative controls include knockout cell lines or tissues that do not express the protein...

  6. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...attend. Societies, such as the American Society for Cell Biology (ASCB) or the American Chemical Society ...for the position. This post on Business Insider nicely summarizes good job fair practice. Career pages..., there is an etiquette that you should follow, nicely summarized in this post on Forbes.com, and here...

  7. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...Specifically, roles in technical support make excellent use of the skills developed by life science PhDs...issue was with a custom-made reagent to manipulate cells (manufactured by one of the larger biotech companies...about novel developments in the fields of gene and cellular therapies.     Additional Resources on the Addgene...

  8. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...questions from the AMA - as you’ll see below, it pretty nicely displays her energetic, truthful and helpful style... training is abundant, helpful, and sometimes excellent. Finally, as I said in the AMA, it’s incredibly...topics to the Addgene blog The blog has many other excellent posts on science career topics as well. Instead...

  9. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... in the genome. These technologies rely on the cellular DNA repair machinery, which can lead to high frequency...understanding of the adenine base editors which can help accelerate the engineering of novel base editor proteins... C (2000) Hugo De Vries: from the theory of intracellular pangenesis to the rediscovery of Mendel. Comptes...

  10. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...annealing to a complementary ssDNA target in the cell.  Only Beta expression is required for recombineering...definitely the easier of the two options, but these cells are prone to mutations and will have more unintended.../Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering ...
Showing: 671 - 680 of 877 results