...delivered as a pooled library via a lentiviral vector to cell lines that either stably expressed Cas9 or that ...almost all genes involved in the production of a cell surface glycan that’s required for ricin uptake....reveals pathways underlying ricin susceptibility." Cell 152.4 (2013): 909-922. Pubmed PMID: 23394947. Pubmed...

...attend. Societies, such as the American Society for Cell Biology (ASCB) or the American Chemical Society ...for the position. This post on Business Insider nicely summarizes good job fair practice. Career pages..., there is an etiquette that you should follow, nicely summarized in this post on Forbes.com, and here...

...Specifically, roles in technical support make excellent use of the skills developed by life science PhDs...issue was with a custom-made reagent to manipulate cells (manufactured by one of the larger biotech companies...about novel developments in the fields of gene and cellular therapies.     Additional Resources on the Addgene...

...questions from the AMA - as you’ll see below, it pretty nicely displays her energetic, truthful and helpful style... training is abundant, helpful, and sometimes excellent. Finally, as I said in the AMA, it’s incredibly...topics to the Addgene blog The blog has many other excellent posts on science career topics as well. Instead...

...annealing to a complementary ssDNA target in the cell.  Only Beta expression is required for recombineering...definitely the easier of the two options, but these cells are prone to mutations and will have more unintended.../Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering ...

... in the genome. These technologies rely on the cellular DNA repair machinery, which can lead to high frequency...understanding of the adenine base editors which can help accelerate the engineering of novel base editor proteins... C (2000) Hugo De Vries: from the theory of intracellular pangenesis to the rediscovery of Mendel. Comptes...

...Fluorescent Protein Plasmids 101: Visualizing Subcellular Structures and Organelles Resources at Addgene.org...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

...Addgene is always looking for new ways to help you celebrate those wins in the lab.   You don’t need...  Quintin Marcelino...

...festivities - however you celebrate! And if you want to see how Addgene celebrates and decorates, follow ...

...co-founder of Addgene. Melina received her Ph.D. in Cell Biology from Harvard University, where she studied...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...