Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 972 results for: Abo;

Showing: 181 - 210 of 972 results

  1. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...little more about Dr. Doench and others at the Broad Institute here Read other blog posts about CRISPR Learn...undergraduate work at Hamilton College. When asked about what got him into science, Doench explained: “I ...career working on miRNAs and RNAi. He first heard about RNAi at his MIT interview during a lecture given...work in Sharp’s lab.  Doench says, “what I loved about [RNAi], ... it was just this complete black box....Doench worked on using RNAi screens to learn more about cancer biology in Ed Harlow’s group at Harvard Medical...technology for its own sake,” but work in true collaboration with scientists asking biological questions....is something that very much resonates with me.” About Addgene’s role in the future, Doench says, Addgene...

  2. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... more about model organisms in the first Five Popular Model Organisms blog post Read more about plasmids...characteristics that make them ideal for studying in a laboratory environment. We’ve covered five model organisms...have been specifically developed to thrive in laboratory settings. One of its most attractive features...minutes, they are perfect for molecular cloning and metabolic engineering.  The E. coli genome has an extensive...address many areas of microbial research like metabolism. Transport, biofilms, and much more. Because ...characterized genome, and is easily maintained in a laboratory environment. What sets Arabidopsis apart from...variation between different plant species and metabolic properties affecting adaptations.  Arabidopsis...

  3. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... potential colleagues study. Think about how you might collaborate or, at the absolute minimum, can appreciate...the first sentence or two what your research is about and what you think you can accomplish. No matter...your seminar accessible. Let the experts ask you about the details and then you can display your deep knowledge...expert's question than to leave everyone confused about your research because you waded too deep into the...simple in principle The first question I usually get about chalk talks is, what is a Chalk Talk? It is a 60...obstacles, alternative approaches, potential collaborations, critiques of your postdoctoral research, etc...effectively convey your message. Key Things to Think about When Developing your Chalk Talk These items should...

  4. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...Revolution Bioengineering. Read their previous blog post about how they started their company here. The first transgenic... foundation of life on earth, and opportunities abound to build better fuels, feeds, foods, and fibers... we hope to inspire and open a new conversation about GMOs.  Challenge #2: Technical obstacles to plant...not understand, and the complicated and unique metabolic processes of plants are being investigated on ...biochemical pathways involved in central anthocyanin metabolism are well understood. Additional work has also...knockouts to better understand developmental, metabolic, and photosynthetic processes. Often the tools...the color-changing flower, and we’d love to hear about the obstacles you’ve faced as a plant scientist,...

  5. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...you access to hard data about what steps are most likely to fail. Our laboratory manager would definitely...can be (there will always be confusion about this though) about the fact that containers are distinct ... Laboratory management software is not a requisite for a functioning lab, but it is for a scaleable lab...techs through the process. The problem? As we said above, any one of these steps can fail but, in our current...gooey contents of said containers, think carefully about how you want your software to treat those encoded...this case, the physical considerations of the laboratory make object composition a smarter choice than...disposable, like a plastic tube. Talk with your lab team about how they would like these things tracked. Terminology...

  6. Tips for Technical Support Calls

    Type
    Blog Post
    ... that long in customer service, I've daydreamed about the ideal customer service interaction - one that...surprised to discover that the most crucial information about a product is printed right on the tube itself! ...best interest of any company to hide information about their products from you, unless they’re knowingly... tongue-in-cheek website.  If you are skeptical about using Google for serious science questions, consider...question asking process Having thought through the above, don’t be afraid send us an e-mail. That’s why we...they cannot (and should not) answer your questions about an Abcam antibody. Have your catalog number ready...faster if we get as much information as possible about the issue. For Addgene support, make sure to mention...

  7. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ... be able to tell that you know about something and are passionate about it.   Maintain a content-useful...like a grad student or post-doc association) Collaboration and teamwork One of the defining characteristics...and many labs in academia benefit from strong collaborations to advance science faster. Help other people...help will be future advocates and references. Collaborate with another lab, in another field maybe, and...and publish Join a lab that has multiple collaborations, even with Industry labs Work on a big project ...project that relies on a division of labor Serve on a committee and do something big like plan a conference ...all the opportunities you can to be communicating about science with others. If opportunities don't exist...

  8. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...to know more about? Interested in doing a postdoc in a presenter’s lab? Want to collaborate with a group...Candidate at the University of Arizona. Perhaps you’re about to present your first scientific poster, but unsure...build your presentation. For example, when thinking about discussing a figure, jot down the question you were...approaches your poster, introduce yourself and ask about his/her background. This will give you a chance ...created for yourself. Hit the key bullet points and elaborate if your audience seems captivated. Expertly navigate...potential questions you may receive. People often ask about the relationship of your work to recently published...ask for clarification. Having them reframe or elaborate their thought will help you to provide an on-topic...

  9. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...plasmid page is nested in. You can see in the example above that the bread crumbs trail starts in the browse... way to find it! But what are all those flames about? Flame logo: The flame that appears beside some...purpose, where can I find more detailed information about the plasmid contents? The middle section of our...The Addgene Vector Database has more information about common vector backbones.  The backbone modifications...inserted into the backbone, and provides information about this gene or DNA element of interest. If the depositor...information: This section provides useful information about how the vector was assembled, and enables requesting... information: This section contains information about the lineage of the plasmid (e.g. if it was derived...

  10. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...could know a little bit about everything or in Jefferson’s case, even a lot about everything. Fast forward...famous discoveries and experiments I’d been learning about for years were usually the products of teams: Watson...very broad term since a project goal could be just about anything. Is the goal of your project to launch ...launch a product? Make an informed decision about something? Fix a problem? Test a hypothesis? Whether you ...it a “project team” or not, most scientific collaborations fall within this category. Unlike a process...to build a team in the first place? Let’s think about some of the advantages of team-based work: Nobody...forward a few hundred years and there are currently about 5.5 million articles in the English version of Wikipedia...

  11. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    ... fine to ask about specific jobs (that is what networking is about), but be upfront about interest in ...scientists-in-training rarely get the opportunity to learn about the myriad of diverse, nonacademic careers that ...with academic scientists who can’t help us learn about the nonacademic sphere where most of us will be ...learning new things. Building new relationships is all about learning new things from other scientists doing ...pursue someone doing a job you wish you knew more about – how do you make a connection? An Informational...alienate connections. If meeting with someone to ask about a job or a particular position at their company,...that this is a good time to ask personal questions about salary, opportunities for advancement, challenges...

  12. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...is the Laboratory Operations Manager at Addgene.  In addition to helping with daily laboratory operations...through barcoded tubes that are scanned into our Laboratory Information Management System (LIMS), a database...available for distribution!    Check Out How We Think about Developing Lab Management Software    The plasmid's...the correct sample. Tracking samples - it's all about location, location, location There are many different...of these samples have the 2D barcodes mentioned above. These barcodes identify what type of sample is ...every barcode on every tube contains information about that tube’s appropriate location. The barcode also...location to your samples, include as much detail about that location as possible. Are there multiple freezers...

  13. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...candidates that are excited about their work and mission.  How do I know what to write about? If you’re struggling...enthusiastic about [something that you love and is important for the job]. Notice that the intros above allow...cover letter is a place to provide helpful context about your candidacy, beyond what is on your resume. It... a great place to proactively address questions about your application. These could be logistical, like...by pointing to your transferable skills. In the above example, if you don’t have experience with ddPCR...but do have experience with qPCR, you could talk about how your experience with qPCR would make it easy...seconds.” That’s exactly what you would want to talk about! You could describe your organizational system, ...

  14. March for Science

    Type
    Blog Post
    ... with a testable hypothesis - an educated guess about how some part of the world functions. Experiments...governments exist in reality and science is how we learn about reality. Formally, a government can be defined ... More concretely, scientists gather information about reality informing people and their governments on...people to rally for science, others are concerned about possible unintended consequences of a march. Three...Democrats more often than Republicans, the March is not about Democrats or Republicans (16). This is a Pro-Science... have more weight than how any one person feels about it. And yet research shows that thoughts on some...politics in meaningful ways, continued conversation about science’s role in government as well as active advocacy...

  15. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...discussions about the pros and cons of different fabrics while we worked. We also had discussions about the difference...grad student. I chose to focus on misinformation about sexual and reproductive health, as the stigma around... misinformation can flourish. I’ve never forgot about all the anxious, whispered questions from friends...the study design or by their preconceived ideas about the controversial medical issues. Participants ...none of the participants had preconceived opinions about “adeolitis.” The participants in my study were ...make reusable cloth menstrual pads while we talked about the environmental impact of disposable products....contexts that shape what people know and believe about the world. It is vital to understand what people...

  16. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...because it required only about 15 minutes of my hands on time and a total of about 3 hours. It was robust...Addgene lab. In this post, I’ll tell you a little bit about what I learned from a New England Biolabs (NEB) ...this, but thought we could learn a little bit more about high throughput DNA prepping by trying out NEB’s... new teaching space at Lab Central to learn all about it. LabCentral is a lab and office space shared ...without breaking their bank accounts. When I heard about how LabCentral aims to enable emerging biotech companies...different biotech companies to interact and learn more about one another. They also provided both breakfast, ...go to a workshop because it will help you think about the most useful pieces of information from the workshop...

  17. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...and Jeffrey Tabor have been working on making E. coli light responsive since 2005, when Tabor was working...these classic systems continue to be optimized by Tabor’s lab, making light-controlled gene expression in...for the computational problem of edge detection. Tabor et al. subsequently engineered a second photosensitive... and highest level of “output” signal, was only about 10-fold, precluding the study of gene expression...-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi...Escherichia coli to see light. Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, ...Addgene. A synthetic genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya...

  18. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ... advantage. It wasn’t until I wrote a blog post about a research paper that explored how scientists connect...saw a request for an Addgene guest blogger post about colony PCR, I signed up immediately. I had used ...science communication skills, while also learning about new scientific developments and helping scientists... found this paper. I wrote an Addgene blog post about it and shared the paper with my lab since one of...project. I was invited to participate on a panel about “Social Media for Science Communication” at the ...Twitter pros as well, like tip #4: “Follow people for about a week and then unfollow those who do not capture...social media to consistently engage with others about your topic(s) of interest. Hopefully through these...

  19. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...Ultimately, problem solving is less about technical know-how and more about how you approach new challenges...transferable skills guide: Problem-Solving. I don’t know about your experience in the lab, but mine was plagued...the job description, but this skill is not just about reacting to technical issues. This skill also comes...I try to clarify what people mean when they ask about “problem solving skills” and look at how scientists...effectively resolves the issue.  If this sounds like about seven different skills to you, you are not wrong...Imagine your problem is just a question you have about the world. You might do some background research...went through your head as you made observations about the issue, assessed potential protocol modifications...

  20. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...Northeastern University, she interviewed Addgenies about their career path. Graduate school: Do I or ...or don’t I? I asked the nine Addgenies about why they did or did not go to grad school. QC Scientist Alyssa...our Financial Operations Specialist, Lucy Hammer, about why she did not go. She told me, “I think that my...Perkins noted that there is a more emphasis today about non-academia and industry jobs. Since there are ...to keep my values in mind as I applied. Thinking about this made me curious. I wondered what my coworkers...view of science. It should be really open and collaborative.” She knew she wanted to leave academia, and...from my co-op. It has been so insightful to learn about different career paths, successes and failures, ...

  21. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...School visited our labs in Cambridge, MA to learn about the wide variety of science careers that Addgenies...s never too early to start networking! Learning about careers at Addgene Afterwards, the students split... was very cool!” and Izzy Shaw, who was excited about “Seeing a real lab!” And several of our visitors...visitors made sure to tell us what we already knew about our Shipping Operations Coordinator: “Kory was awesome...awesome!”. The students also got to learn about intellectual property from our Tech Transfer team members...are offered with a biology major.” After hearing about many Addgenies’ career paths, Michelle Healy said...work to help improve K-12 education? Let us know about your educational events in the comments section ...

  22. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...carrying a synthetic gene drive were to escape the laboratory, the drive could eventually spread through the...mistake would necessarily extend far beyond the laboratory and seriously damage public trust in scientists... can spread through populations of transgenic laboratory organisms but not wild organisms. For example...synthetic sequence will only spread in transgenic laboratory populations with that sequence2. Both methods...Reproductive confinement involves working with laboratory organisms that can't reproduce with wild ones...confinement seeks to keep the organisms in the laboratory. It varies by organism, but your local biosafety...population and immunizes it against the first drive. Laboratories interested in building candidate gene drives...

  23. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...When you think about going to a scientific conference, you may think about sitting amongst a sea of chairs...After learning about using sound to visualize bacteria in the lab, I was excited to learn about creative approaches... at different ultrasound frequencies. Read more about ‘Using ultrasound to image bacteria in vivo: acoustic...Protein Resources and get started!  I learned a lot about creative ways to do science communication and outreach...outreach - if you'd like to learn more about Science communication check out these SciComm blog posts.... Methods for Bacterial Genome Engineering Learn about Science Communication using Twitter  Resources ...

  24. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...scientists to make genomic alterations, bringing about a revolution in genome engineering, with new techniques...available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins  An...delivery of RNPs is electroporation (A in the figure above), which generates pores in the cell membrane, allowing...as lipid-mediated transfection (B in the figure above), are in the early stages of development. David ...proteins harboring receptor ligands (C in the figure above), which result in the internalization of Cas9-gRNA...show promise for in vivo studies, little is known about the immunogenicity and stability of the Cas9 protein...and purification, which will require additional laboratory reagents, equipment, and expertise. Final thoughts...

  25. How to Write a Scientific Review Article

    Type
    Blog Post
    ...didn’t have to worry about having to hunt down sources later on.  A comment about writing: initially I...exists. Once you pick a topic, try to be specific about exactly what aspect of the field you plan to review...themes in the field and to learn what people cared about. I also used reviews to get a list of research papers...highlighted a few dozen things that I didn’t know about. Instead of reading a paper and getting my bearings...them. No really, just start writing! Don’t worry about grammar or formatting or continuity. Also don’t ...worry if you feel like you don’t still know enough about the topic. Just get as many words down on the page...between office hours and experiments. A comment about citing: I wasn’t sure if I should cite reviews or...

  26. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...The Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in... of Zimbabwe, Zimbabwe Dr. Idah Sithole-Niang (above, left) is an Full Professor in Biochemistry with... CRISPR effect observed in bacteria. Learn more about the different CRISPR applications in plants.     ...Rodrigo Arturo Rivera Solís, Associate Professor (above, far right), thrives to make affordable biosensors...detect glyphosate via H2O2, one of the reaction’s metabolites. One useful genetically encoded H2O2 probe is... to the Addgene blog for updates and future collaborations. Many thanks to all of the awardees, all who...Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out...

  27. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ... will also serve as a good mentor. To read more about what makes a good mentor, see the previous post ... and personnel management?  If you are thinking about a non-academic path, will the PI support this path...Medical School researcher Connie Cepko to learn about her mentoring style. How can I find out if an advisor...time with the potential advisor and don’t be shy about asking hard questions. Talk to as many lab members... past lab members. Most scientists love to talk about their past lab experiences and will be willing to...members, neighbors or alumni give you information about the PI that concerns you, don’t just brush this ...Mentors by @drmellivora, an excellent blog post about workplace bullying featured on the always interesting...

  28. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...Cell tracing in zebrafish with Zebrabow Before the generation of Zebrabow (Pan et al., 2011), Brainbow ...crossing of Zebrabow animals to an inducible Cre line. The Schier group developed Zebrabow as a system...by injecting Brainbow at the single-cell stage. Zebrabow, or “zebrafish brainbow,” is a collection of transgenic...are introduced using a plasmid construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream...downstream of a ubiquitous promoter. Zebrabow is an extremely versatile technology as recombination can be...an introduction to live cell imaging Learn more about the origin of the Brainbow system Visit our fluorescent... Addgene’s fluorescent protein collection Learn about fluorescent tools for in vivo imaging ...

  29. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...a successful negotiation and collaboration. We’d love to hear more about your experiences and opinions...can get away with using computer modeling and collaborations rather than using animals, then that’s a cost...associated with old equipment, like service repairs, labour costs, and loss of time, can exceed the cost of...Everything is negotiable. Try asking for discounts, collaborations, access to internal award applications, etc...This is a non-profit organization. Therefore, collaboration and community is the ultimate value proposition...Happy Sciencing, Damien   Damien Wilpitz is a laboratory research management consultant and coach in Boston...research scientists create productive and manageable laboratory environments. His business skills enhance his...

  30. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... communication, and in particular communication about experimental methods and approaches. It’s very easy...everything will be fine. It won’t. When we hear about issues with reproducibility, time and time again...the problem comes down to inadequate information about what the original researchers actually did. Admittedly...Communication comes in many forms—from transparency about data to allow others to see what you actually found...fits together to make for a transparent and collaborative research environment. Journals as enforcers ...of methods sections, including clear information about reagents, and why other publishers and organizations... in reproducibility jail and more effective collaboration to drive the progress of science. Sounds like...
Showing: 181 - 210 of 972 results