We narrowed to 345 results for: Ada

1. Antibodies 101: Isotypes

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   ... constant domains. This switching helps B cells adapt during the course of an immune response since it...

2. Antibodies 101: Monoclonal Antibodies

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   ...10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced...

3. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   ... in much of the world, we have found ourselves adapting to a new way of doing things. These new processes...

4. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   ...RspWYL1 increases both targeted and collateral RNA degradation by RspCas13d. RspWYL1 also increased EsCas13d...

5. Luminescent Imaging with Nano-lanterns

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   ...Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. Improving Nano-...

6. Top Requested Lentivirus and AAV of 2016

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   ...PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell ...

7. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...group inserted sgRNAs targeting various innate and adaptive immune genes into pXPR_053. They expressed this...

8. Targeted Mutagenesis with EvolvR

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   ...Nature 560.7717 (2018): 248. PubMed PMID: 30069054. Sadanand, Saheli. "EvolvR-ing to targeted mutagenesis."...

9. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   ... dsRNAs target specific mRNA transcripts for degradation. Despite how easy these experiments are to physically...

10. Communicating Your Science With Help From ComSciCon

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    ...in science communication from across the US and Canada convene at ComSciCon's annual flagship workshop...

11. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    ...cell differentiation (a cell type important for adaptive immune responses, which flies do not have!) (4...

12. Improved Plasmid Maps Powered by SnapGene

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    ...’re not at your computer. Maps, sequences, & downloadable .gb & .dna files Once you’ve identified a plasmid...

13. Plasmids 101: Screens vs. Selections

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    ...and consider these questions to save yourself a headache later on. Enhancer and suppressor screens These...

14. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    ...years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research...

15. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    ...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although...

16. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ...Repression and Activation. Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...

17. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    ...ardor and attended to with diligence.” - Abigail Adams, 1780 There are potential mentors all around you...

18. All in a Twist: dsRNA

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    ...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...

19. Visualizing Protein Turnover In Situ

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    ...the combined effect of protein synthesis and degradation. These processes control the abundance of proteins...

20. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    ...cells promote oxidative stress leading to the degradation of one key enzyme (among others): caveolin 1,...

21. Summer SciComm: Preprints

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    ...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...

22. Troubleshooting Your Plasmid Cloning Experiment

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    ...provided by the major companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but...

23. Plasmids 101: Cre-lox

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    ...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...

24. Hot Plasmids February 2024

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    ...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission.   Find...

25. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021.   Let me leave...

26. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...indirect ELISA (more details below) can also be adapted to a competitive format. Figure 2: Overview...

27. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well as unique...

28. Adenoviral Vector Production and Troubleshooting

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    ...which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated gene delivery...

29. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...triggers a cascade of events that results in the degradation of the target mRNA and silences its expression...

30. Science communication: 9 Strategies to Get Your Foot in the Door

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    ...to Zooniverse. The Natural History Museum has downloadable resources to enable you to set up your own citizen...

31. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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    ...Office Ben Corb - Director of Public Affairs at ASBMB Adam Fagen, PhD - Director, Early Career Scientist Segment...

32. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of...

33. Mouse Modeling, Part 1: Genetically Engineered Mice

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    ...mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research...

34. Experimenting in an Open Source lab: from CRISPR to Cats

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    ...Project One such space is the Victoria Makerspace in Canada, which I belong to. The team at the Victoria Makerspace...

35. Hot Plasmids and Viral Preps - May 2021

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    ...validating this method through monitoring GFP degradation, this protein compartment was used to optimize...

36. Plasmids 101: Protein Expression

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    ... grown in well-defined media and can be easily adapted to fermentation allowing for large-scale, stable...

37. Truncated gRNAs for Regulating Gene Expression

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    ...interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator...

38. Plasmids 101: Knockout/Knock-In Plasmids

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    ...them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "...

39. Plasmids 101: 5 factors to help you choose the right cloning method

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    ...Gateway cloning protocol is fairly flexible and adaptable to cloning different types of DNA, such as cDNA...

40. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    ...colocalization analysis example is given in Figure 2 (adapted from 4) - Dunn et al. 2011 provides an in-depth...