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We narrowed to 801 results for: MAK

Showing: 241 - 280 of 801 results

  2. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ...Two plasmids that can be used to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently... plasmid prep protocol, it cost less than $10 to make 1000 lanes worth of the 100 bp or the 1 kb ladder... than commercial ladders. Are you interested in making your own DNA ladder? You can find the Tan Lab’s...Protocol Learn How to Analyze Your Restriction Digests Make Your Agarose Gel Other Resources Read about Gel...

  3. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...calculator can do the math for you and it’s simple to make adjustments for the type of tissue culture dish ...dish and number of dishes used. Here’s to making AAV packaging a little easier! Click here to download the... spreadsheet: We hope that this calculator will make it easier to plan your AAV packaging experiments...AAV Packaging and Transfer Plasmids Here New to Making AAV? Check out Addgene’s Step-By-Step Protocol ...

  4. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...available the potential mentor is. If the person makes time for you, as needed, this is a strong indicator...time and resources. You typically have 4-5 years to make discoveries and publish papers to position yourself...most exciting story I can tell with my data?  Can I make better slides that more effectively tell a story...present your research. Face to face meetings can make favorable impressions on potential manuscript or...minimal processing, and low in empty calories will make you feel better and help maintain your health. ...

  5. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...twenty graduate student volunteers worked hard to make this Saturday event a success, a true testament ...communication gap between scientists and the public and help make science more transparent. Second, SITN helps graduate...intelligible scientific resources. SITN hopes to make DayCon a yearly event and to expand the conference...is a great idea and a group of people willing to make that idea a reality! How do you share your science...

  6. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...Tools for Making Scientific Graphics by Beth Kenkel Find a round-up of tools you can use for making scientific...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim Goedhart Guest..., and fusion location, there are many choices to make. 2. Prime Editing: Adding Precision and Flexibility...

  7. Editor's Choice, August 2016

    Type
    Blog Post
    ..., I’m picking two posts that describe methods to make single base edits in DNA. The first, contributed...a thorough walkthrough of how you can use PCR to make small sequence changes to plasmid DNA. This an oldie...CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at target sequences specified by...informative post. Interestingly, this technology may make some uses of Site Directed Mutagenesis by PCR obsolete...

  8. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    ... a job you wish you knew more about – how do you make a connection? An Informational Interview is a great...relationship building through Informational Interviews. Making Your informational interviews productive Informational...phone or video call. If you do decide to use Skype, make sure to follow the rules of successful, remote conversations...many good lists of questions on the internet. To make the most of the opportunity, use a written list ...

  9. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...Tip 1: Use color coding, labels, and post-its to make finding your most used protocols & references easy...for you) and place them on an easy to reach shelf. Make sure all notebooks are visibly labeled on the spine... tabs (for different experiments or projects) to make it easier to know where to start your search. Then...track the revision history. Now you’ll be able to make updates to protocols in real time. You may also ...

  10. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    ...contributed by our depositors, Addgene strives to make it easier for you to find the plasmids and publications...and the original depositor. Our citations feature makes it so that, when you cite a plasmid from Addgene...who may be working on similar projects and should make it easier for them to find your work later. We also...process. Special collections pages In an effort to make it easier for you to browse through the many plasmids...

  11. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...collection, the fluorescent proteins are, which makes mixing and matching a relatively easy proposition...Davidson lab for the last four years. “Researchers can make their own target if it is not already made for them...For more information on choosing a backbone and making fusions, see this blog post from Joachim Goedhart...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many...

  12. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy to target multiple loci - a concept called...expressing multiple gRNAs on the same plasmid, you’ll make sure that each cell that gets the plasmid contains...increases the chance that all the edits you want to make will happen.  There are many reasons to multiplex...constructs with multiple promoter-gRNA cassettes, making them advantageous for small capacity vectors like... mammalian option described below, plasmids for making polycistronic gRNAs are also available from the...

  13. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... the absence of guide RNA, preventing Cas9 from making off-target cuts.   Pro tip! X-ray crystallography...base. In Figure 5, we can see why: two arginines make base-specific hydrogen bonds to the guanines, but.... Residues R1333 and R1335 from the CTD (yellow) make base-specific hydrogen bonds to guanines of the ...The HNH domain, which cuts the target DNA strand, makes few contacts with the rest of Cas9 and is highly...BioRender.com.   The exact conformational changes that make Cas9 such a valuable laboratory tool are still an...

  14. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...adopting a few meaningful changes in the lab could make a big difference in our global impact as scientists... environmental impact, but we can all do more to make research more environmentally friendly. Adopt one...Universities and companies have the potential to make a huge impact directly on waste, but also on culture...bring ideas to your workplace and you could end up making a huge impact on how waste is handled. A lot of...

  15. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...mapping These tools are for viewing, editing or making plasmid maps, but can also analyze and annotate...also has a suite of molecular biology tools and can make plasmid maps. Free for academic users. Serial Cloner... blank spaces, from the text of a DNA sequence, making it ready to copy and paste into other applications... Addgene blog: Learn how the CRISPR software matchmaker can help you choose the best CRISPR software ...

  16. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ...video. Don't worry - no pipettes were harmed in the making of this photo!  Here, I was also given a mixture... understand the importance of using analytics to make decisions on content strategy. I really believe ...different best practices, create an email strategy, and make the occasional TikTok. I was also responsible for... my time here comes to an end, my worries about making connections with coworkers seem, in retrospect,...

  17. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...medical use. Find More Optogenetic Tools Like pDawn  Making cassava plants resistant to cassava mosaic virus...this drought tolerant plant, Dr. Robertson aims to make use of the CRISPR/Cas9 technology to engineer plants...for example this yeast cloning kit) she intends to make yeast capable of desaturating fatty acids. Identification...Associate Professor (above, far right), thrives to make affordable biosensors that detect herbicides, such...

  18. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...one of the most challenging [parts of my job], so making it a success feels like a real accomplishment.”... to take over hosting them. Since then, webinars make up a larger part of Hans’ workload. “I am currently....” A few other tips: “Learn HTML, write a lot to make sure you don’t hate it, and be flexible. The best...could be featured in future career blog posts. And make sure to subscribe to the Career Channel of the blog...

  20. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    ...for imaging a protein’s entire life-cycle. This makes it nearly impossible for scientists to use GFP to...large, dim, and permanently attached to a protein, making them hard to amplify and image for long periods...in vivo. These probes however can be expensive to make, hard to deliver into living systems, and often ... proven to fold and function in living cells. To make these scaffolds specific to different targets, the...

  21. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    ...problems through good software design. But what makes software good? Fortunately, that question has been... task, we envision what the user wants to do and make the path to accomplishing their goals as intuitive...the most important controls front and center, and make specialized controls available when needed. SnapGene...For a coding sequence feature that may be used to make fusion genes, detection needs to occur even if one...

  22. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...you believe your idea has potential to make a difference, then make sure you’re able to answer every possible... Start with your network You've spent your life making connections and finding mentors -- well, it's time...discuss each project and business decision before making it, to have an internal review system.  On top ...

  23. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...method limits screening to a few hundred worms, making the detection of rare events difficult. Dickinson...resistance and the Roller movement phenotype. To make these markers easy to remove after injection, they... to generate a self-excising cassette (SEC). To make it possible fluorescently label proteins in C. elegans...fluorescent protein fusions, this system could be used to make many other genome modifications, including targeted...

  24. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ..., as it can edit near to or far from PAM sites, making it less constrained by PAM availability than other...within the base editing window, while prime editors make a specific edit defined by the pegRNA. In cases ...the non-edited strand, they can cause the cell to remake that strand using the edited strand as the template... These variants are worth testing if you want to make your prime editing as efficient as possible. ...Some examples include: TwinPE: two prime editors make complementary edits to opposite strands of the DNA...

  25. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...are able to make when they have access to shared materials, and then pay it forward by making the new materials...has been able to rapidly propagate this strain, making it available to scientists around the world. This...research by removing logistic hurdles to continue making materials available rapidly to scientists (Nature...

  26. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...generating/replenishing kits, is to find new ways to make lab processes more efficient. Being in the Boston...there because both LabCentral and Addgene aim to make scientific advancement easier. NEBNext Ultra FS ...we did at the workshop (the kit is mostly used to make new libraries as opposed to replicating them like...new without spending a lot of time developing and making space for the techniques ourselves. As you probably...

  27. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...pombe (fission) and S. cerevisiae (budding). Yeast make for an excellent model organism for a few reasons...phaffii is highly efficient at protein secretion, making harvesting recombinant proteins a cinch. This is...it doesn’t secrete its own endogenous proteins, making the recombinant protein its major secretion. Beyond... removing methanol from the media. This feature makes the strain tractable for peroxisomal studies on ...

  28. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ... strategies can make socially distanced work more creative. An example could be making lab meetings more...without leaving Zoom. The annotate function can also make the screen-sharing experience more interactive. ...: “Science is increasingly done in large teams, making it more likely that papers will be written by several...

  29. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...Christopher Voigt and Jeffrey Tabor have been working on making E. coli light responsive since 2005, when Tabor...systems continue to be optimized by Tabor’s lab, making light-controlled gene expression in E. coli easier...the response regulator encoded in the genome. To make the system less bulky and easier to use with other...Schmidl et al. chose to optimize these systems to make them more tunable and user-friendly. They experimented...

  30. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...the earliest) or a functional one. Pick one that makes sense for your skills, and the job you are applying...seconds on a resume when they first look at it. Don’t make them have to guess what you are capable of. You ...might know a thing or two about computer modeling. Make sure to focus on these skills. The only exception... yet for that dream job. Also, many universities make their job postings and salary expectations public...

  31. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ... digest tips and tricks: The following tips will make it easier for you to obtain a useful and informative... recognition sequence. Most enzyme manufacturers make High Fidelity versions of the endonucleases and/...before loading them. The glycerol in the buffer will make sure your sample settles in the gel well and the...wrong! Quantify your DNA. Loading too much DNA will make it difficult to obtain crisp bands and analyze the...

  32. Hot Plasmids - May 2022

    Type
    Blog Post
    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...Parton Lab recently created a flexible system that makes use of pre-existing transgenic lines, termed BLITZ...system provides researchers better flexibility and makes the approach more accessible, as it reduces the ... be packaged into an AAV system. dCas9-SSAP will make the editing of kilobase-scaled sequences more efficient...

  33. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...efficiency. Check out Addgene’s blog to learn about making your own competent cells and our protocols page... encodes a green fluorescent protein gene which makes the bacterium fluoresce green. Comic by Maya Kostman...Lytic phages hijack the bacterial hosts machinery to make more viral particles. Eventually the cell lyses ...bacterial chromosome and allows the donor bacterium to make a small “bridge” or sex pilus that attaches to the...

  34. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ... explore small-scale opportunities where I could make mistakes and learn from them. I started at my previous...Beyond Academia: Stories and Strategies for PhDs Making the Leap to Industry." He successfully transitioned...Resources  Resources for aspiring managers Books: The Making of a Manager by Julie Zhuo Radical Candor by Kim...Managing vs. Leading Management for Scientists: What Makes a Good Leader Anyways?  Transferable Skills Guide...

  35. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...will be a positive asset throughout your career? 1. Make scicomm practice a priority The good news for young...of the work you presented may also be unclear. 2. Make the audience your primary focus When writing a paper...address that 'why' question effectively, you need to make your audience the focus of every sentence you write...know this? How will they react to it - what will it make them think? Does this lead to the conclusions I ...

  36. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...feature, you can compare different Cas enzymes to make sure you are getting the desired features and cutting...and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving database, and... to it! The CasPEDIA Consortium will, of course, make efforts to keep it updated with new entries and ...Structural Biology. https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I., Iranzo, J., et al. (2019)...

  37. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...think. So, if you’ve ever wondered what goes into ‘making virus’ or what gag and pol stand for, we demystify...recombinant adenovirus is missing the endogenous E1 gene, making it replication deficient. This is typically supplied... some of the plasmids in our collection. Want to make a vector construct on your own? No problem, we are...discussed are compiled for reference below. Happy virus making!  References and Resources Kulanayake, S., Tikoo...

  38. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...value. Types of biosensors So now that we know what makes up a biosensor, we can discuss what flavors of sensors...difficult to achieve in whole organisms, but AAVs make it possible. In AAV systems, different serotypes...expression and downstream monitoring. Nervous about making a virus for your biosensor? Don’t be! We have you...viral resources and safety tips. Still worried? We make virus with some of our most popular biosensors already...

  39. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ...sometimes called “VUS”) function is unknown. How can we make a decision for each patient if the majority of information...high-throughput manner and therefore push us closer to making cancer precision medicine a reality. Challenges...have worked closely with our Addgene colleagues to make these available to the scientific community. Functional... Institute of Harvard and MIT and is focused on making precision functional genomics a reality for future...

  40. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...Zetsche, B., Gootenberg, J., Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015... diagram below for an example of how this works. Make sure to order 5’ phosphorylated oligos or treat ...single plasmids are generally more efficient. Pro-Tip Make sure to reverse the orientation of the crRNA’s direct...
Showing: 241 - 280 of 801 results