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  2. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ... Techniques Find Additional Posts on Plasmid Technologies Resources at Addgene.org Learn about Popular...Plasmids in the Repository Learn about New Plasmid Technologies in our Hot Plasmid Articles ...

  4. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...at all levels.      Jargon is field-specific terminology or language. This can refer both to terms that...that are only used in the field and terminology that is widely used but has a very specific use-case (definition...

  5. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub 2016 May 2. PubMed PMID: 27136078. Find...Resources on Addgene.org Check Out Other Argonaute Technologies from the Doudna Lab Browse the CRISPR Collection...

  7. Hot Plasmids - January 2023

    Type
    Blog Post
    ...transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9...monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:e43322. doi:...

  9. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ... NEB gave a brief presentation describing the technology. Then we split into pairs to actually try out...with a technician from Neon Therapeutics, a biotechnology company that uses neoantigen biology to treat...

  10. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...Biotechnology is no longer just for those working in academia or industry. At BosLab, we identify ourselves...student at Boston University studying mammalian immunology, and in his free time, he is pursuing a plant...

  12. Science Career Options

    Type
    Blog Post
    ...Writer Meteorologist Journal Editor Textile Technologist/Designer     Fieldwork and traveling Many...desired, as roles are becoming more reliant on technology, and good computer skills will give you an advantage...

  13. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...developers’ willingness to make their CRISPR technologies available to the academic research community...Addgene, we’ve helped distribute many of these technologies in plasmid form and are proud to have facilitated...

  17. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...allow a protein to be more effectively used in a technology or as a therapeutic.  A (very) brief history ... mostly accessible to experts. That many GCE technologies have idiosyncrasies presents a significant barrier...

  18. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...Schambach A. Gammaretroviral vectors: biology, technology and application. Viruses. 2011 Jun;3(6):677-713...An Overview of the Application of Viruses to Biotechnology. Viruses. 2021 Oct 14;13(10):2073. DOI: 10.3390...

  19. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...visible part behind these changes: the backend technology that powers the new website search. Tackling ... resources, blog posts or reagent items.  The technology behind Addgene’s new search  Like millions of...

  21. Hot Plasmids - February 2022

    Type
    Blog Post
    ...live-cell imaging, single-molecule tracking, immunolabeling of fixed cells, Western blotting, and more....future genetic screens.   DeWeirdt PC, et al. Nat Biotechnol. 2021. doi: 10.1038/s41587-020-0600-6. The Penn...

  22. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... is one of the latest developments in CRISPR technologies.  It’s a simple and effective method with multiple...CRISPR–Cpf1 Asing a Single crRNA Array." Nature Biotechnology 35.1 (2016): 31-34. Pubmed PMID: 27918548. 2...

  24. Hot Plasmids - May 2022

    Type
    Blog Post
    ... for example).  Given that the liver is an immunologically active organ, this advance is likely to afford...Kaufman, et al, 2022.    This new barcoding technology would allow researchers to avoid the off-target...

  25. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...you have any questions or thoughts about this technology, leave them in the comments below.  References... Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Jun;32(6):569-76. doi: 10.1038/nbt.2908...

  27. To Each HIS Own

    Type
    Blog Post
    ...Proteins with a Novel Metal Chelate Adsorbent. Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff...growth factor I from culture medium of E.coli. Bio/Technology. 1987, 5:379-382. Bornhorst JA and Falke JJ. ...

  28. Antibodies 101: Conjugation

    Type
    Blog Post
    ...References and resources References Bendall SC, Nolan GP, Roederer M, Chattopadhyay PK (2012) A deep profiler... profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016/j.it.2012.02.010 Dennler...

  29. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ... the lab to be using words for their field or technology in a consistent way. Just creating a lexicon ...first weeks or months when I was learning the terminology of a new field. Annotate: When creating important...

  31. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34(5) (2016): 528-30. PubMed PMID: 27088723....

  32. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...about how they would like these things tracked. Terminology and language are tricky Names for things will...running and more time working on interesting biotechnology code.There are valid technical reasons to choose...

  34. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...bleach (plastic) or ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol...

  35. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ... most PVDF membranes recommend re-wetting with methanol before rinsing with wash buffer, as dry PVDF won...Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks...

  39. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...team will communicate with your organization’s technology transfer office or other authorized office directly... ship the samples to Addgene and contact your technology transfer office about the MTA. Pro Tip! Download...

  40. 6 Tips for Grant Writing

    Type
    Blog Post
    ...network of your peers. Furthermore, using new technologies to solve a problem can allow you to approach...other scientists that we forget that some of the terminology we use routinely isn’t common to non-scientists...
Showing: 241 - 280 of 547 results