We narrowed to 550 results for: nol

...female. HEKs are generally cultured as an adherent monolayer, though derivatives have been adapted for growth...Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI: 10.3389/fbioe.2021.796991...

...our network.  We started with the idea of a biotechnology investment fund that would apply a percentage... biotechnology...

...materials available rapidly to scientists (Nature Biotechnology 2020). The continued success of repositories...j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020...

... NEB gave a brief presentation describing the technology. Then we split into pairs to actually try out...with a technician from Neon Therapeutics, a biotechnology company that uses neoantigen biology to treat...

...was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University. In this second...guest blogger Jon Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has had nearly ...

...Writer Meteorologist Journal Editor Textile Technologist/Designer     Fieldwork and traveling Many...desired, as roles are becoming more reliant on technology, and good computer skills will give you an advantage...

...developers’ willingness to make their CRISPR technologies available to the academic research community...Addgene, we’ve helped distribute many of these technologies in plasmid form and are proud to have facilitated...

...lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor, sufficiently suppresses basal... tagged proteins, a common problem of the AID technology (Yesbolatova et al., 2019). Find the mAID tagging...

...Ernesto Valdivia Silva, Universidad de Ingeniería y Tecnología (UTEC), Peru Julio Ernesto Valdivia Silva is ...synthetic biology resources and microfabrication technologies to generate a self renewable sensor, a bacterium...

...presented chronologically. With a resume however, most often people choose a reversed chronological style ...

...transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9...monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:e43322. doi:...

...Biotechnology is no longer just for those working in academia or industry. At BosLab, we identify ourselves...student at Boston University studying mammalian immunology, and in his free time, he is pursuing a plant...

... for example).  Given that the liver is an immunologically active organ, this advance is likely to afford...Kaufman, et al, 2022.    This new barcoding technology would allow researchers to avoid the off-target...

...multiprotein complexes.  Fig. 2. Multiplex immunolabeling with subclass-switched recombinant antibodies...monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:e43322. doi:...

...live-cell imaging, single-molecule tracking, immunolabeling of fixed cells, Western blotting, and more....future genetic screens.   DeWeirdt PC, et al. Nat Biotechnol. 2021. doi: 10.1038/s41587-020-0600-6. The Penn...

...was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University. In my previous...guest blogger Jon Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has had nearly ...

... is one of the latest developments in CRISPR technologies.  It’s a simple and effective method with multiple...CRISPR–Cpf1 Asing a Single crRNA Array." Nature Biotechnology 35.1 (2016): 31-34. Pubmed PMID: 27918548. 2...

...allow a protein to be more effectively used in a technology or as a therapeutic.  A (very) brief history ... mostly accessible to experts. That many GCE technologies have idiosyncrasies presents a significant barrier...

...Schambach A. Gammaretroviral vectors: biology, technology and application. Viruses. 2011 Jun;3(6):677-713...An Overview of the Application of Viruses to Biotechnology. Viruses. 2021 Oct 14;13(10):2073. DOI: 10.3390...

...you have any questions or thoughts about this technology, leave them in the comments below.  References... Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Jun;32(6):569-76. doi: 10.1038/nbt.2908...

...CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's... require access to next generation sequencing technology. These libraries are lentiviral in nature, ...

...visible part behind these changes: the backend technology that powers the new website search. Tackling ... resources, blog posts or reagent items.  The technology behind Addgene’s new search  Like millions of...

...about how they would like these things tracked. Terminology and language are tricky Names for things will...running and more time working on interesting biotechnology code.There are valid technical reasons to choose...

...it’s obvious that CRISPR is a ground-breaking technology that’s already had a huge impact on research ...effects on various biological disciplines, the base technology itself is constantly improving. Cas9 variants...

...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34(5) (2016): 528-30. PubMed PMID: 27088723....

...References and resources References Bendall SC, Nolan GP, Roederer M, Chattopadhyay PK (2012) A deep profiler... profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016/j.it.2012.02.010 Dennler...

...Proteins with a Novel Metal Chelate Adsorbent. Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff...growth factor I from culture medium of E.coli. Bio/Technology. 1987, 5:379-382. Bornhorst JA and Falke JJ. ...

...Tools for studying bioelectricity Dyes and other technologies are now available for fluorescent reporting ...synthetic bioengineering." Current opinion in biotechnology 52 (2018): 134-144. PubMed PMID: 29684787. Oviedo...

... the lab to be using words for their field or technology in a consistent way. Just creating a lexicon ...first weeks or months when I was learning the terminology of a new field. Annotate: When creating important...

...network of your peers. Furthermore, using new technologies to solve a problem can allow you to approach...other scientists that we forget that some of the terminology we use routinely isn’t common to non-scientists...

...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids...-Cas Cpf1 nucleases in human cells." Nature Biotechnology (2016). PubMed PMID: 27347757. Find plasmids...

...Fengshan Forest Park. As a national science and technology park, it enjoys preferential governmental policies...high-tech development zone so many science and technology companies and start-ups choose to locate there...

...Aug 3, 2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017 for all incoming plasmids...Partnering with seqWell, we are using the plexWell technology to easily and quickly create Illumina sequencing...

...updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins... applications: natural brakes for CRISPR-Cas technologies. Nat Methods 17:471–479 . https://doi.org/10.1038...

...the Associate Director and Head of Gene Editing Technology at Beam Therapeutics, used a previously developed...activity and therapeutic application. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0491-6 Additional...

...team will communicate with your organization’s technology transfer office or other authorized office directly... ship the samples to Addgene and contact your technology transfer office about the MTA. Pro Tip! Download...

...bleach (plastic) or ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol...

...efficiency and off-target effects compare to existing technologies, and their use for HDR editing and mouse-model...compared head-to-head with existing CRISPR delivery technologies, Nanoblades had higher rates for editing efficiency...

...first blog post in this series, I’ll highlight terminology you should be familiar with before working with...Cre is expressed.  Several variations of this technology can be used to customize the effects of this ...

... most PVDF membranes recommend re-wetting with methanol before rinsing with wash buffer, as dry PVDF won...Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks...