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We narrowed to 784 results for: SCR;

Showing: 301 - 350 of 784 results

  1. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... drug discovery by big pharma (i.e. HTRF and LanthaScreen) (Degorce et al., 2009), measuring intracellular...are critical in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET...it all together The FRET donors and acceptors described above can be paired together to measure a wide...

  2. Deep Dive: qPCR

    Type
    Blog Post
    ... by abundant transcripts, leading to falsely lowered levels for less abundant transcripts. However, adding...can even be used to detect a single copy of a transcript. But this sensitivity can sometimes cause as ...

  3. The 12 Days of CRISPR

    Type
    Blog Post
    ...Noninvasive assessment of gut function using transcriptional recording sentinel cells! 🎵 On the 11th day...gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR...

  4. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...various CRISPR applications (such as visualize, screen, activate, and repress) on Thursday. We’d also ...from nickases to base editing to pooled library screening. It also has information for planning your CRISPR...

  5. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    ...populate your “PubMed filter list” to the left of your screen. That’s it! Next time you log-in to your PubMed...Scientific Review Article Tips for Improving Your Next Manuscript Resources on Addgene.org Learn How to Order...

  6. CRISPR Cheat Sheet

    Type
    Blog Post
    ...used to increase or decrease expression of a gene. Screen: A library of gRNAs with a CRISPR nuclease target...cell population. The resulting mutant cells are screened for phenotypes of interest. Limitations of CRISPR...

  7. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...your experiment* Functional data, if the report describes a functional vector such as a channelrhodopsin...site as well as the target region, if the report describes a retrograde serotype An abstract that summarizes...

  8. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    ... Just click on the search bar at the top of the screen and type in what you’re looking for. After you ...know about new articles on the Addgene blog by subscribing to instant alerts below.  ...

  10. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, they found that fusing dCas9 to...to an Mxi1 repressor domain repressed transcription of HSP90 an essential C. albicans gene. Read the ...

  11. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    ...plasmid page. Check out the purple arrow in the screenshot below to see what I mean. Additional features...increase the length of the list (purple oval in the screenshot below). You can also use the "Search Table" box...

  14. Reflections and Looking Ahead

    Type
    Blog Post
    ...supporting the tools in our collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub... to learn more about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi,...

  15. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...communication between all members of a team. As described here in a 2012 Intuit blog post, a study coming...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series...

  18. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...released the MORF collection, a comprehensive transcription factor library, in lentiviral vectors. At 3500...conduct robust investigations into the human transcriptome. The MORF library is only one of 22 libraries...

  20. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...The system consists of dCas9, the mammalian transcriptional repressor Mxi1, and an optogenetic tool that...editing. Using several rapid and cost-effective screening methods, David Liu’s lab identified and validated...

  21. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ... interacting partners and perform whole animal screens for small molecules that block the neurodegenerative...and pathways to target in future small molecule screens. He plans to work with a partner at IRCCS Besta...

  22. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ... greater than any fluorescent protein earlier described. “Until our proteins, there were lots of fluorescent...illumination with far-red light. As the researchers described in Nature Communications last year, “The capability...

  23. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... and variable spacer sequences. The array is transcribed and processed into crRNAs, each including one...from Addgene!   RNA crRNA: “CRISPR RNAs” are transcribed from the CRISPR array and include a spacer and...

  24. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...These sensors generally follow the same pattern described above - a sensing domain induces changes in a ... such as protein or DNA binding, enzyme or transcriptional activation, conformational changes, translation...

  25. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...better. More transcription factor binding sites in a promoter lead to higher transcriptional activation....

  26. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...Lentiviral vector design. B) Strategy for leak-free transcriptional control. C) Iterative transduction workflow...of biosensors could be used in high throughput screening for agrochemical compounds that have the potential...

  27. Fostering communication in remote collaborations

    Type
    Blog Post
    ... that everyone can access data and drafts of manuscripts and grant applications in real-time so they can...figure out are timelines for experiments and manuscripts, who is responsible for which parts of the project...

  28. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...showing the effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists...plasmids are available as a collection, facilitating screening across the transducerome, to gain a more complete...

  29. Hot Plasmids - October 2022

    Type
    Blog Post
    ...called JEDI-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21... 1: Summary of the approach and outcomes of the screen for improved genetically-encoded voltage indicators...

  30. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series...

  31. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...find the vector that fits your experiments, I've described below some of the most frequently requested vectors... bacteria and a mammalian selection cassette to screen for positive transfected cells. The addition of...

  33. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...ready-to-use viral preps   Improved cell-free transcription/translation system to examine biosynthetic pathways...pathways  By Gabrielle Clouse Recently, cell-free transcription/translation systems (also referred to as TX-...

  35. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...multicolor labeling is a powerful solution for discriminating between neurons for light microscopy-based ...expression level of XFPs, most likely by suppressing transcription. It is critical to express a minimal amount ...

  38. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...generally refer to them as the “interested public.” Subscribers to Scientific America would be considered the...scientists in (roughly) the same field, often through manuscripts, seminars, or posters. It is important to note...

  39. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...protein of interest. 4. Plasmids 101: Blue-White Screening Getting a lot of empty vector contaminants in ...Addgenie Jessica Welch explains how Blue-White screening could be a potential solution. 5. Cpf1: A New ...

  41. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies...

  42. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...Coordinator’ came up at Abcam. Unlike other job descriptions for marketing positions posted on recruitment...would be able to carry out a yeast-2-hybrid library screen; instead I thought they would focus on “Has this...

  44. Hot Plasmids - January 2023

    Type
    Blog Post
    ... knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm presents an attractive...! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol...

  47. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...directly transformed into E.coli for plating, screening and generation of sgRNA. Generation of sgRNA libraries...Assembly and Other Long-Homology Based Cloning Methods Screen Plasmids by Colony PCR Addtional Resources on ...

  48. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...phrase gram negative or positive being used to describe a species of bacteria, but what does it actually...What is a plasmid? Plasmids 101: Blue-white screeningPlasmids 101: Common lab E. Coli strains  ...

  49. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...scientific communities, but usually I only get to scratch the surface of different fields. This meeting has...

  50. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...that lab notebook from 2 years ago. The free subscription to Lab Archives allots for 25 MB of space per...only need to skim the alphabet on the side of the screen to find the sequence for your enzyme of interest...
Showing: 301 - 350 of 784 results