We narrowed to 438 results for: %s
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TypeBlog Post...Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER...Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S, Graham BS, Callewaert N, Schepens B, Saelens X, McLellan...Egloff P, Hutter CAJ, Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA (2020) Generation...
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Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post....00990-19 Liljeruhm, J., Funk, S. K., Tietscher, S., Edlund, A. D., Jamal, S., Wistrand-Yuen, P., … Forster...https://doi.org/10.1007/bf00428249 Chan MCY, Karasawa S, Mizuno H, Bosanac I, Ho D, Privé GG, Miyawaki A, ... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...technology! Resources and References References Orguc, S., Sands, J., Sahasrabudhe, A., Anikeeva, P., & Chandrakasan...https://doi.org/10.1109/EMBC44109.2020.9175600 Park, S., Loke, G., Fink, Y., & Anikeeva, P. (2019). Flexible...C8CS00710A Sahasrabudhe, A., Rupprecht, L. E., Orguc, S., Khudiyev, T., Tanaka, T., Sands, J., Zhu, W., Tabet... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post...Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., & Scher... Scher, D. S. (1994). Isotype choice for chimeric antibodies affects binding properties. In Journal of... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...they think they are. References Berns KI, Adler S (1972) Separation of two types of adeno-associated...Maynard LH, Smith O, Tilmans NP, Tham E, Hosseinzadeh S, Tan W, Leenay R, May AP, Paulk NK (2019) Fast-Seq... . https://doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke R, Andres-Mateos E, Xiao R, Maurer... -
Plasmids 101: TOPO Cloning
TypeBlog Post... colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia virus DNA topoisomerase...coli is sequence specific." Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10104-8. PubMed PMID: 1658796...synthesis using vaccinia DNA topoisomerase. Shuman S. J Biol Chem. 1994 Dec 23;269(51):32678-84. PubMed... -
Microbiology Resources
TypeCollection...constructs for S. cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae... -
CRISPR Antimicrobials
TypeBlog Post... et al. used a phagemid system to target virulent S. aureus, a common hospital-borne infection. Two rounds...phagemid treatment decreased the proportion of virulent S. aureus from 50% to 11% in just 24 hours. ... to target the sensitive population. Yosef et al.’s proof-of-concept work targeted two distinct beta-lactam...antibiotic-resistant bacteria.” Proc Natl Acad Sci U S A. 9;112(23):7267-72. (2015). PubMed: PMID 26060300... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...10.1096/fj.01-0995fje. Tan, E., Chin, C. S. H., Lim, Z. F. S., & Ng, S. K. (2021). HEK293 cell line as a platform...Plasmids and Resources References Shaw, G., Morse, S., Ararat, M., & Graham, F. L. (2002). Preferential... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog Post...out, might depend more on the individual sequence(s) than on how well that sequence fits into the five...Knight, T. F., Thomas C. Evans, J., & Lohman, G. J. S. (2018). Comprehensive Profiling of Four Base Overhang...Potapov, V., Bilotti, K., Pokhrel, N., & Lohman, G. J. S. (2022). Rapid 40 kb Genome Construction from 52 Parts.... B., Bilotti, K., Cantor, E. J., & Lohman, G. J. S. (2020). Enabling one-pot Golden Gate assemblies of... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...26225354. PubMed Central PMCID: PMC4515362 Robinson, W. S., Pitkanen, A., & Rubin, H. (1965). The nucleic acid...Rous sarcoma virus: Initiation of synthesis with 70 S viral RNA as template. Journal of Molecular Biology.../0022-2836(73)90277-5. PubMed PMID: 4355596 King, S. R., Duggal, N. K., Ndongmo, C. B., Pacut, C., & Telesnitsky...18094172. PubMed Central PMCID: PMC2258930 Hu, W.-S., & Temin, H. M. (1990). Genetic consequences of packaging... -
Fluorescent Protein Guide: Biosensors
TypeCollection...657. Douglas Kim , GENIE Project Calcium GCaMP6f/m/s calcium sensors for imaging neural activity (Constitutive...Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or Vcre Comprehensive... encoded fluorescent sensor. Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Ca 2+ indicators based...genetically encoded sensors. Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Amy Palmer Zinc Cytosolic...single GFP-linked biosensors. Proc Natl Acad Sci U S A. 2008 Jan 8. Wolfgang Dostmann cGMP (cyclic GMP)...Schaffer collateral synapses. Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. Katalin Torok Glutamate...neurons of Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2019 Aug 1. Alexander Gottschalk Voltage Near-infrared... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...plasmid deposit. Figure 2: Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information...plasmids. Figure 3: If submitting plasmid(s) online (left), choose to submit as published or unpublished...with an asterisk are required. If submitting plasmid(s) using a spreadsheet (right), click the “Download ...following descriptions for your plasmid: Encodes insert(s) A plasmid with one or more genes, protein coding...if you’d like to distribute your bacterial strain(s) through Addgene. Description – please indicate the...Figure 7: Enter information about the gene/insert(s). Step 3: Cloning Information Please enter the name... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...Genesis 34, 1–15 (2002).PubMed PMID: 12324939. 3. S. E. McGuire, G. Roman, R. L. Davis, Gene expression... PMID: 21356876. 5. J. Chow, Z. Marka, I. Bartos, S. Marka, J. C. Kagan, Environmental Stress Causes Lethal...PMID: 22205518. PubMed Central PMCID: PMC3574576. 7. S.-L. Lai, T. Lee, Genetic mosaic with dual binary transcriptional... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post...References Cele S, Gazy I, Jackson L, Hwa S-H, Tegally H, Lustig G, Giandhari J, Pillay S, Wilkinson E, ... -
Performing In Vivo CRISPR Screens Using the FITS Approach
TypeBlog Post...Milling, L. E., Markson, S. C., Tjokrosurjo, Q., Derosia, N. M., Streeter, I. S. L., Hickok, G. H., Lemmen... -
Visualizing Your Plasmid Request Data
TypeBlog Post... data only, information on requesting PI(s) & organization(s) is not shown on the dashboard. Key features... -
Allen Institute for Cell Science Plasmid Collection
TypeCollection...Hookway, C., Ludmann, S. A., Mueller, I. A., Yang, R., Horwitz, R., Rafelski, S. M., & Gunawardane, R.... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...861-72. PubMed PMID: 18035408. 3. Takahashi, K. and S. Yamanaka, Induction of pluripotent stem cells from...temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci U S A, 2011. 108(34): p. 14234-9. PubMed PMID: 21821793... of canonical Wnt signaling. Proc Natl Acad Sci U S A, 2012. 109(27): p. E1848-57. PubMed PMID: 22645348...10): p. 1836-46. PubMed PMID: 22976355. 34. Wang, S., et al., Differentiation of human induced pluripotent...p. 82-94. PubMed PMID: 23329641. 36. Jopling, C., S. Boue, and J.C. Izpisua Belmonte, Dedifferentiation...): p. 987-1000. PubMed PMID: 3690668. 40. Ferber, S., et al., Pancreatic and duodenal homeobox gene 1 ... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...PubMed Central PMCID: PMC3193421. 4. Hacein-Bey-Abina, S., et al., LMO2-associated clonal T cell proliferation...5644): p. 415-9. PubMed PMID: 14564000. 5. Seki, T., S. Yuasa, and K. Fukuda, Generation of induced pluripotent...(4): p. 718-28. PubMed PMID: 22422317. 6. Diecke, S., et al., Recent technological updates and clinical...transformation-deficient Myc. Proc Natl Acad Sci U S A, 2010. 107(32): p. 14152-7. PubMed PMID: 20660764...