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Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

Learn about what sequencing technologies the quality control team at Addgene uses to maintain a high standard of plasmid and viral vector quality.

This post covers loading controls and normalization of semi-quantitative western blots. We cover housekeeping proteins and total protein loading controls.

Looking to use adenoviral vectors in your research? This post introduces you to the adenovius!

Find out more about the different homology directed repair pathways and how to design HDR templates for CRISPR editing.

Open science resources like Addgene's Data Hub increase reproducibility, reduce validation and optimization timeframes, and drive science forward.

Learn about how to identify and develop creativity in the lab - a skill that's valuable in and out of the sciences!

Pseudotyping is a method use to introduce a viral envelope protein from another virus to restrict or broaden host cell. Get the what, why, how of pseudotyping in this article.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

Learn how site specific recombinases like Cre-lox and FLP-FRT can be combined for complex control over gene expression in neuroscience experiments and more.

Use site directed mutagenesis to insert small mutations into your plasmid of interest and follow these tips for a smooth process with easy validation.

This post, written by the creator of the CRISPRecise kit, explains how to use it to minimize off-target effects in CRISPR genome editing.

Optogenetics aren't just for neuroscientists! In this post, we review optogenetic tools for cytoskeleton and membrane control in cells.

With the limitations of qRT-PCR based COVID-19 testing, scientists develop CRISPR-based diagnostic tools for COVID-19.

Learn about CRISPR plant base editors for plants

Learn how biotin is used in a variety of molecular biology experiments and makes it easier for researchers to label and purify macromolecules of interest.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Check out some of the new and useful tools recently deposited in the Addgene repository!

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.