...libraries is well suited to large-scale de novo assemblies of short constructs like plasmids. The plexWell..., this has resulted in high quality NGS-based assemblies for over 3000 plasmids in the Addgene collection...of Toronto. He is an author of 20+ scientific publications and patents.   ...

...apoptosis reporter, ZipGFP, which utilized the self-assembling split GFP (GFP1-10 and GFP11) to increase fluorescence...animals. To do this they modified a different self-assembling split GFP (GFP1-9 and GFP10-11) by flipping a...rectify this the Shu lab first improved the self-assembling capability of sfCherry approximately 20 fold ...

...funded by the public and performed by researchers who received their educations from public schools and ... BSc in Anatomy, the University of Otago had established the Centre for Science communication and I was...

...Collaboration and creativity are pivotal in writing publications/grants too. As described by several works (Wuchty...collaborative writing and this recent article published in PLOS Computational Biology (Frassl et al., ...approaches can lead to frustration, delay of publication, or even the termination of a project.” Take ...

...Imaging Improving Well-established High-throughput assays Improving Well-established Biological sources...describing the cloning of firefly luciferase was published in 1985, several studies utilized luciferase as...

... the example of our Antibody Guide, which was published alongside our new antibody services in the spring...through an editing process and final check before publication.   Do research! Nearly everything we write entails...final review to catch those pesky typos, and hit publish. Making content is an iterative process. We are...

... now. Enter the SAM pooled library In a paper published in Nature in December, Konermann and her colleagues...Zhang lab for GeCKO, a lentiviral CRISPR library enabling genome-scale, knockout screening). As the Zhang...pooled CRISPR libraries, also available at Addgene, enabling systematic investigation of the cellular consequences...

...Addgene blogpost and more recently in a paper published in F1000Research on November 2016. Due to the ...were at institutions where no data had been made publicly available (below). However, on November 22nd a...compelled at the state level, in the case of some public institutions) to cancel plans to raise salaries...

...journalism.” She also interned two days a week on a public radio show. After graduating with a master’s, Susan...complete a certificate in Strategic Communications and Public Relations at the University of Washington and the...opportunities, such as writing for a student-led publication, are more readily available and it’s often easier...

...Albert Einstein College of Medicine has recently published techniques to follow translation in living single...presented a novel technology, back then still unpublished, that allowed the visualization of specific de...combination of FUNCAT and PLA PLA was originally published by the Söderberg lab in Sweden as a method for...

...the most similar to yours. Take a look at the publications cited on the manufacturer’s website, which may...highlights a variation of your application, a publication successfully used it in a similar way as you ...safer bet than one that has never been used in a publication. Talk with your peers. If someone in your field...

...are publicly available, including those found at Addgene! Figure 1: Strategy for assembling the...your experiments. Figure 2: Strategy for assembling barcoded combinatorial gRNA libraries. (Adapted...

...purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types... PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “...PubMed PMID: 26258682. Find plasmids from this publication at Addgene. 3. Kirchhofer, A., et al. “Modulation...

...contributed by Johnna Roose. This post was originally published on Johnna's New Under The Sun Blog and is part...is science’s way of validating work prior to publication, it doesn’t mean that there are no mistakes or...Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript by Maaike Pols, PhD, ...

...for the administrative side of the grant to be established. Take time to read and fully understand the RFA...and develop novel technologies! If data from a publication or preliminary data suggests the feasibility ...will empower the world” tone that differs from publication writing and may be challenging to master at first...

... in situ hybridization experiments. A recent publication [1] from the Sánchez lab describes the plasmids...the deposit) are described in the Sánchez lab publication [1] so that the students performing these experiments..., is available from Addgene. If you use these published protocols and want to share pictures, feedback...

...Originally published Aug 3, 2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017... that contain synthesized regions that have no publicly available reference sequence or plasmids containing...these plasmids are returned as one or two partial assemblies. If we obtain an NGS result that is useful, but...

...significant clinical efficacy against a form of blindness known as Leber’s congenital amurosis (LCA). This...RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable until 2008. ...decline in vision with gene therapy in childhood blindness." New England Journal of Medicine 372.20 (2015...

...landscape facing all scientists, both young and established, as they embark on or continue their careers ...Advisor MIT Washington Office Ben Corb - Director of Public Affairs at ASBMB Adam Fagen, PhD - Director, Early...science and in the intersection between science and public policy. Follow David on Twitter @driglar.   References...

...PubMed PMID: 27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver, Benjamin P., et ...PubMed PMID: 27347757. Find plasmids from this publication at Addgene. 3. Zetsche B, Gootenberg JS, Abudayyeh...PubMed PMID: 26422227 Find plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi...