We narrowed to 214 results for: DAP
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TypeBlog Post...student, I have relied more and more heavily on adapting my smartphone to help me with my research in terms...
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Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...disappointing, but there are many creative ways to adapt and take advantage of the new conference format.... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...disrupting ribosome-mRNA complexes. Recent work has adapted this technique to monitor local translation and... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...for non-commercial reuse. There should be ways to adapt the game to each a variety of subjects at many different... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...without changing them. Thus, it has been widely adapted for a wide variety of applications: base editing... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post... that exist online, but this approach has been adapted for the identification of physical materials that... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...using this kit is: Fragmentation and end repair Adapter Ligation Clean up/Size selection Amplification ... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...and RFP or the Sedat Quad filter set, designed for DAPI / Fluorescein / Rhodamine / Cy5 (e.g. Semrock's)... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol...protocol was established using 293T cells but can be adapted to alternative cell lines. Workflow Timeline Day... -
Hot Plasmids - February 2022
TypeBlog Post...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA... -
Antibodies 101: Isotypes
TypeBlog Post... constant domains. This switching helps B cells adapt during the course of an immune response since it... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post... in much of the world, we have found ourselves adapting to a new way of doing things. These new processes... -
A Deep Dive into BioBuilder
TypeBlog Post...with synthetic biology lab kits and lesson plans adapted from her own MIT curriculum. Drawing on current... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...cell differentiation (a cell type important for adaptive immune responses, which flies do not have!) (4... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. Improving Nano-... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...group inserted sgRNAs targeting various innate and adaptive immune genes into pXPR_053. They expressed this... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...Comparison of different Cas enzymes using CasPEDIA. Adapted from Adler et al. You can also search by ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although... -
Western Blot
TypeProtocol...protocol uses a dry transfer device but can be adapted for wet and semi-dry transfer approaches. Last ...