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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 773 results for: Dos

Showing: 121 - 140 of 773 results

  2. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that should...

  4. March for Science

    Type
    Blog Post
    ...presented in this article are those of the author do not represent a formal stance taken by Addgene or... them. Scientific realities limit what policy can do and, in this way, science has always been related... hypotheses mount yet policies and public opinion do not seem to take note. For example, 91% of active...’ ability to uphold their side of the bargain and do the research required to inform the public (8–13)...party while alienating the other While scientists do identify as Democrats more often than Republicans...contact information for your senators all you need to do is select your state. State Legislatures: To find...

  5. Antibody Guide

    Type
    Collection
    ...comes from. Then consider the following questions: Do you need polyclonal or monoclonal antibodies? Will...direct or indirect detection method? If indirect, do you already have secondary antibodies available in...have identical features and epitopes, to ensure you do not validate the same antibody multiple times. You...antibodies will also have a source species. What conjugate do you want to use? Most secondary antibodies will be...buy small aliquots of many different antibodies and do batch validation up front rather than risk delaying...

  6. CRISPR Plasmids - Parasites

    Type
    Collection
    ...guides against 8,158 predicted protein-coding genes. Do you have suggestions for other plasmids that should...

  7. AAV Viral Preps

    Type
    Collection
    ...on the Function section pages above. You can also do a search of viral preps and filter by the serotype...

  8. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...And do remember to breathe!)   Public speaking as a transferable skill The worst thing about doing public...before it, I don’t think I would have had the nerve to do what I did. Until that panicked, split-second decision...people won't notice, or won't remember and those who do are very likely to be sympathetic because public ... notes instead of written speeches. When you’re doing all that, even if it’s exceptionally difficult for...

  9. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ...causing conformation changes in the AraC dimer. In doing so, the dimer shifts from one that represses transcription...expressing your gene of interest, all you have to do is co-transform pBLADEONLY. That way, a previously... less heterogeneity in the population and you can do all of these more spatially confined experiments ... as those requiring reversibility that you cannot do with AraC,” says Di Ventura. BLADE activates transcription...

  10. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    ...this very blog (you’ll have to let me know how I’m doing a few months down the line!) and to visit scientists...with none of my old friends to fall back on. While doing and presenting undergraduate research at BU, I realized...knowledge. In addition, this position allows me to do another thing I’ve always thoroughly enjoyed: travel...support academia but the skills researchers gain doing PhDs (i.e. technical writing, teaching, presenting...

  11. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...they do well and where they need to improve, and they’re also clear about how the person is doing overall...would your plan be? If you could do the thing all over, what would you do differently? Beware of indirect...

  12. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...how little pre-work most PhD students and postdocs do to ensure the advisors they choose will be ones that...are a common reason for scientists to change labs – do as much as you can to ensure a good fit in advance...ensure the PI will be a good role model and teacher. Do you admire her management style? Does he have ethical...academic bullies are hard to stop – the best thing to do is not go into their labs in the first place. The...

  13. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ...laboratories this holiday season want the work you are doing to have an impact, to move science forward, or perhaps... perhaps even society. One obvious way to do that is and has been to publish in journals with a high “...access on scientific impact Partly, the story has to do with online accessibility. It is now about as easy...

  15. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...researchers do these things with nefarious goals in mind. Indeed, it’s not always obvious that we’re doing things...reagents with the confidence that they aren’t simply doing an experiment wrong. However, even simple procedures...researchers to share data thereby enabling you to do modified analyses on published data if you need to...confirms their hypotheses even if most other scientists do not. These scientists with positive results have ...biases and goals from effecting our experiments. How do we engender better experimental design? The summation...transparent about the reasons we alter these designs if we do so. The most extreme version of this (and possibly...

  16. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...wages, overtime tracking? What are institutions doing? Institutions are now preparing to comply with the...postdocs resource on what institutions are actually doing (using the list of 340 institutions with postdocs...institutions that have not yet announced what they are doing, however, may face greater challenges. Current salary...most of) MIT and the University of Colorado are doing, and are checking with Yale that they are raising...institutions they're thinking about applying to, are doing - for example, your benefits may be changing. Please...of time and a lot of work - but they were able to do so on their own terms. They must feel very relieved...

  17. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... consider the order of your narrative. Scientists do this all the time, you just need to figure out how... deliver a great public presentation if you are doing it for the first time? Practice talks also help ...sakes, label all graphs and data professionally). Do remember the fine points of visual aids. Here’s a...say you will get back to them with an answer, and do so if you can. 9. Resist the urge to say everything...of talk length, and then I cut a few more slides. Do not run over your time—the audience’s attention will.... One good topic or story is all you need, if you do it well.  Resist the urge to show all or even a lot...

  18. 6 Tips for Grant Writing

    Type
    Blog Post
    ... hear you saying “We know this already!” But, you do need to be able to research and write your application...money so you need to make it clear that you will be doing your research in a group or facility that is a world...it would be helpful for them to know what we are doing and why. I always ask kindly friends and family ...to my area, a line about the research I will be doing, and a line about what I hope to discover. This ... you get some critical insight into what you're doing wrong. All this helps to improve your application...

  19. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ... blastp searches do not filter low complexity regions by default, meaning that you do not have to remember...compared to other types of BLAST searches. If you do not know the exact reference sequence for your result...truncations. A primary advantage of blastx is that you do not have to decide on a reading frame for your sequencing...pairwise_sequence_alignment_psa Happy BLASTing! Do you have any tips for using BLAST to confirm your...

  20. Science Career Options

    Type
    Blog Post
    ...leaving academia, but don’t quite know what you do want to do or even what careers are available to scientists... Science in the News Don't stumble into a career, do your research Once you have identified possible careers... part of team? Will you be coordinating projects? Do you need computer programming skills? Other things...
Showing: 121 - 140 of 773 results