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We narrowed to 638 results for: Erf

Showing: 121 - 140 of 638 results

  1. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...do anything. Viruses are complicated and they perform complex biological tasks, both of which are factors...you’re unsure about the integrity of your virus, perform a diagnostic restriction enzyme digest and compare...transfected packaging cells. This check can be performed routinely and without disrupting the flow of your...viral vector. This antibiotic selection can be performed approximately 72 hours after transfection and ...Another method of ensuring viral production is to perform PCR against components of the viral vector using...

  2. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...even more.) However, none of the tools we found performed batch guide design, batch primer design or batch...transformation (illustrated in the graph below) is performed because weak gRNAs (CFD < 20) bind almost not ...strong gRNAs (CFD > 80) bind almost as much as perfect gRNAs (CFD = 100). CFD scores (which range between... cut and the insert, the more poorly the guide performs HDR. Optimal mutation in-depth One the most sophisticated...donor RNA Design primers Review and order reagents Perform your wet lab experiment Analyze the data For more...

  3. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...Camera 4K. It’s wonderful and simple to use, but when you need to juggle filming and performing on camera, ...laptop raised on an ironing board, giving it the perfect height (Fig. 2). We recorded video using a simple...celebrating Massachusetts Nonprofit Day, which was the perfect opportunity to test shooting over Zoom, everyone...talent could just focus on their interview or performance. By this point, towards the end of the summer...

  4. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...leaves a “scar” in the chromosome, sometimes interfering with expression of surrounding genes. However...and Prather, 2015). Both of these systems are powerful, but they rely on exogenous enzymes, require multiple... markers for allelic exchange. To do this, we performed the following modifications to pDS132 (Philippe...Hospital where he is now an attending physician. He performs post-doctoral research in the lab of Matthew Waldor...PMC3748948.  Khetrapal, Varnica, et al. "A set of powerful negative selection systems for unmodified Enterobacteriaceae...

  5. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...status as a Biosafety Level 1 microbe makes it a perfect candidate for introductory teaching and research...generation time of around 20 minutes, they are perfect for molecular cloning and metabolic engineering...a model organism has its perks, it is far from perfect. One of the most limiting attributes of rats is...Zhang, C., & Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional genomics...genomics study in bacteria with superior performance. Nature Communications, 9(1). https://doi.org/10.1038...

  6. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...use a unique and powerful high-throughput screen to select variants based on performance metrics specifically...Near-infrared (NIR) fluorescent proteins promise better performance for in vivo use and deep-tissue imaging. Vladislav...for screening candidate biosensor variants for performance under two-photon fluorescence lifetime imaging...produce a temperature sensor that significantly outperforms previous designs. B-gTEMP is excitable under ...

  7. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...use. Or you can really capitalize on the dye’s performance and use super-resolution or single-molecule techniques...fluorescent ligand and see the results right away. Or perform pulse-chase experiments by sequentially adding ...priority, SNAP-Tag or HaloTag have been shown to perform differently for different ligands (Wilhelm et al...super-resolution imaging, HaloTag gives better performance than SNAP-tag (Erdmann et al., 2019). Check out...new ligands for these systems, enhancing their performance and ease of use. With so many possibilities from...

  8. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...user-deposited data detailing how an antibody performed in an experiment.   Figure 1: The front page ...contains experimental data showing how the antibody performed in the submitted application, along with a brief... results of an immunocytochemistry experiment performed with the anti-Lhx6.1 antibody.    For a more detailed..., and a short description of how the antibody performed.  Figure 5: Results for the data report shown...

  9. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...phenotypic readout, similar mapping could be rapidly performed by targeting all potential components of a signaling...antibodies. Antibodies could further be used to interfere with the identified surface proteins, and such...available through Addgene. This means you could perform a screen with one library from Addgene, and then... in vitro, but there is enormous potential in performing in vivo screens, studying genes affecting cellular...the information that you have input in the web interface, making it convenient for you to later identify...

  10. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...unwind the DNA and check for sequence matches; a perfect guide RNA will do you no good if there is no adjacent...tolerates mismatches in the next 8-10 bases after perfect pairing in the seed sequence.   Figure ...lab mutated the residues in this loop to produce SuperFi-Cas9, a high-fidelity variant that still retains... CRISPRing!   Molecular graphics and analyses performed with UCSF ChimeraX, developed by the Resource ...Bravo, J. P. K., Liu, M.-S., Hibshman, G. N., Dangerfield, T. L., Jung, K., McCool, R. S., Johnson, K. ...

  11. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...fluorescent biosensor. Image from Chesterfield et al., 2020     Chesterfield et al., ACS Synthetic Biology... and physiology in vitro and have emerged as a powerful model for understanding human physiology, development...the Rawlins Lab have compiled a new toolkit for performing genetics studies in organoids. The Organoid EasyTag...

  12. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...format not only enables you to discuss a person's performance or what has been learned, it helps to plan effective...of indirect language You must be honest about performance problems. While giving corrective feedback isn... or use indirect language to talk about real performance issues. Watch this little video to learn more...don’t know how you actually feel about their performance. Giving honest and helpful feedback gets easier...

  13. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...primers that contain the regions of homology. A powerful resource for planning an NEBuilder HiFi DNA Assembly... which is freely available online. Its simple interface can guide you through both experimental and primer...design results in covalently joined, scarless DNA – perfect for transforma­­­tion or as a PCR template. Some...designing primers with your desired mutation, performing PCR to generate the mutation and linearizing ...

  14. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...We all know that yeast is a powerful eukaryotic model organism – its genome is easy to manipulate, it...expression   K. phaffii first gained attention for its powerful promoter of a gene important for methanol usage...essential for sequestering certain enzymes and performing specific oxidative reactions. They are found ...predominantly uses non-homologous end joining (NHEJ) to perform DNA double strand break repair instead of homologous...

  15. Hot Plasmids - January 2023

    Type
    Blog Post
    ...constructs Anti-GFP [N86/8R] ready-to-use antibody Hyperfolder YFP CRISPR-Csm: A novel RNA targeting system...Another reason is that some antibodies may just not perform as well in one application as another, so it is...eLife.43322. PMID: 30667360; PMCID: PMC6377228. Hyperfolder YFP- a chemically stable tool for microscopy ... The Liu and Petsko labs recently engineered hyperfolder YFP (hfYFP), a FP well-suited for the demands...

  16. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...you need quickly.  Through the years, we have perfected processes to curate the data our depositors share...down, and zooming out — with an intuitive user interface.   The team approached the issue from multiple... search, our updated search engine is now more powerful, intuitive, and searches across all of our content...built our new search engine atop Elasticsearch, a powerful open-source search engine that can scale to search...

  17. Clear and Accessible Writing

    Type
    Blog Post
    ...details of collecting the purified AAV–that the interfaces between layers are blurred after centrifugation...the syringe needle is inserted bevel-up at the interface between the 40% and 60% layers. Putting them all...iodixanol gradient after ultracentrifugation. The interfaces between layers are muddled due to the passage...collect purified AAV, insert a syringe at the interface of the 40% layer containing the virus and 60% ...

  18. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...treat cells with many different drugs and then perform an assay to look at an outcome. Your scientific...libraries targeting all known genes. This is a perfect example of an unbiased, reverse screen – all known...capacity. Drug screens, on the other hand, are often performed as biased forward screens. A given panel of drugs... phenotype. For example, a drug screen may be performed on a cell line possessing a patient mutation to...

  19. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...computation, but they will serve you well as you perform analysis after analysis after analysis… Treat ...background on other analyses that have inspired you to perform this analysis. Having a clearly stated, specific...the scripts and input files that will be used to perform each task. Conclusion: Always, always, write ...know that the file is the output of the analysis performed in experiment 1. Use camelCase: In camelCase...

  20. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...antibody. Perform a second series of wash steps. Activate the signaling molecule and perform the assay...antibodies to bind to a primary antibody without interfering with the hypervariable region, where an antibody...sample with the primary (unconjugated) antibody. Perform a series of wash steps to remove any unbound or...
Showing: 121 - 140 of 638 results