We narrowed to 270 results for: FAS
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TypeBlog Post...the two best-performing variants as iGluSnFR4f (fast deactivation) and iGluSnFR4s (slow deactivation)...and speed, so you can design, test, and innovate faster than ever. Plus, we've already taken them for a...
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Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post... later became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR...ligands (Wilhelm et al., 2021). While HaloTag reacts faster for some dyes, others, especially some nonfluorescent... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...formatted output. Surprisingly only 2 tools provide FASTA output. A few notable tools allow the user to save...dependency is a severe limitation, but the tool is fast enough that, if you’re engaged in heavy CRISPR design... -
Delivery Methods for Generating iPSCs
TypeBlog Post...reprogramming period and the plasmid will get diluted faster when cells are actively dividing. The typical drawbacks...less time to complete. Morphology is a simple and fast to evaluate. iPSCs should form compact colonies ... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...actuator pair. Ideally, indicators should possess fast voltage sensing kinetics and have no spectral overlap...named CheRiff, exhibits high light sensitivity and fast kinetics. By combining both the voltage indicator...visualize the Broccoli-tagged RNA. This technique is faster and less labor intensive than northern blotting... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...generate ABE8e(TadA-8e V106W), which edits ~590-fold faster than the TadA from ABE7.10 without increasing off-target...preceded by Gs Richter et al., 2020 ABE8 590x faster adenine base editing than ABE7.10 Gaudelli et... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...-based MAGE The Nielsen lab’s CRMAGE system is a fast, multiplexable method that combines CRISPR and recombineering-based...possible with efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...small protein is over-transferring, try using the faster semi-dry transfer, which works better for small...low levels of proteins. In this case, it might be faster to run the same amount of protein in every lane... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...Chronos, is blue and green light-excitable and has faster kinetics than those of other channelrhodopsins....optimizing protein expression and purification in a fast, efficient and affordable way. Scholz et al.., BMC... -
New Coomassie Protocol Video
TypeBlog Post...Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol... -
Trends in CRISPR and SynBio Technologies
TypeBlog Post...data on plasmid deposits and distirbution for these fast moving fields. Addgene is a global nonprofit plasmid... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog Post...genes, plasmids ship out approximately three weeks faster, as no license, or license fee, will be required... -
Bringing Renewable Energy to Addgene
TypeBlog Post... cannot be washed or reused/repurposed in some fashion. Addgenies swapping out printer cartridges can ... -
CRISPR Cheat Sheet
TypeBlog Post...CRISPR material for you! The CRISPR field moves fast. That’s why we’re bringing you a brand new third... -
Depositor Week 2020 is Happening Now. Join us!
TypeBlog Post...science along? We’ll also take a look back on some fascinating Addgene history that you might not have known... -
Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More
TypeBlog Post...the ability to generate mutant microbial strains faster than ever before. 29:54 - 31:25 : Nature's defenses... -
New Viral Vectors - Spring 2025
TypeBlog Post...services allow scientists to conduct experiments faster, skipping the preparation steps of production, ... -
Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis
TypeBlog Post...biological devices. Chapter 6: - Making research go faster through Open Access. Chapter 7/Outro: Tom’s Involvement... -
Bringing Sustainable Practices to the Lab: Easy Action Items
TypeBlog Post...energy consumption in the lab Here are a few easy and fast things you can start doing right away to reduce ... -
Addgene Stands with the Scientific Community
TypeBlog Post...Help Despite these challenges, Addgene remains steadfast in our mission to accelerate research and discovery...