We narrowed to 268 results for: GFP

1. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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   ...endocytosis of a fluorescent dye and transduction of GFP-expressing AAV particles we did not observe any difference...

2. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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   ...or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9,...

3. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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   ...transcription activation systems, such as LexA/p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell...

4. Plasmids 101: What is a plasmid?

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   ...cell Plasmids 101: Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of...

5. Hot Plasmids - October 2022

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   ...Addgene’s western blot for the myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the Applications...

6. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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   ...strain transformed with pBLADE expressing superfolder GFP instead of mCherry under the PBAD promoter. They ...

7. CRISPR Challenges: Standardization and Homology Directed Repair

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   ...PubMed Central PMCID: PMC5495063 Kan et al. use a GFP-BFP conversion system to assess precision genome ...

8. New Norepinephrine Indicators: nLightG and nLightR

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   ...contains circularly permuted green fluorescent protein (GFP), and nLightR uses a red fluorescent protein, mApple...

9. Antibodies 101: Affinity Tags

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   ... (FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry, etc.). Fluorescent tags are primarily used...

10. New Viral Vectors - Winter 2025

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    ...serotype (multiple) rAAV-CAG::FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics Scott Sternson New viral prep ...Recombinase Ian Wickersham New viral prep pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 AAV2 Recombinase James M. ...Recombinase James M. Wilson New serotype pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 AAVrg Recombinase James M... prep AiP14033: pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033) AAV-PHPeB Optogenetics... prep AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) AAV-PHPeB Optogenetics... prep AiP15140: pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) AAV-PHPeB Optogenetics...

11. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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    ...using a bacterial expression system such as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant...

12. Year of the Camelid: Antibody Style

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    ...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation.  sdAbs ...

13. 10 Great Guest Posts We're Thankful For

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    ...Favorite Fluorescent Protein Oligomerizes in Cells GFP oligomerization can lead to misleading artefacts ...

14. Using Addgene's New Viral Service for Your Research

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    ...also have lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can...

15. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    ... University with David Goldstein’s lab, will use GFP-ATP1A3, FLAG-ATP1A3, and ATP1B3 plasmids to explore...

16. Sleeping Beauty Awakens for Genome Engineering

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    ..., hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain two SfiI...

17. Choosing Your Perfect Empty Backbone

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    ...using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. pPD80_08) and following the expression pattern... allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating transiently...

18. Brain Armamentarium

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    ...James M. Wilson 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...Viviana Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...Tasic Melina Fan 221463-AAV1 pAAV_BiPVe4_eGFP AAV construct expressing eGFP driven by chandelier cell-targeting...enhancer. Alias: pAAV_WDC0004_eGFP Gordon Fishell James M. Wilson 221463-PHPeB pAAV_BiPVe4_eGFP AAV construct...construct expressing eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_eGFP Gordon Fishell...

19. New and Upcoming Viral Vectors - September 2019

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    ...collection is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465) are now available...our entire AAV inventory. Our new AAVs include: EGFP-expressing AAV for serotype testing Calcium sensors... testing AAV, which are small (20 ul) samples of EGFP-expressing AAV packaged in various serotypes. This...recently released these controls: pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad...broad, neuronal expression. AAV pCAG-FLEX-EGFP-WPRE (51502-AAV5): The CAG promoter directs ubiquitous expression...interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5 and 112677-AAVrg): The EF1a promoter...mCherry in the absence of Cre, and expresses nuclear EGFP in the presence of Cre. Biosensor AAV (calcium ...

20. Another Pathway into Cells: iTOP

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    ...Neuronal stem cells transduced with iTOP resulting in GFP expression. Image courtesy of Diego D'Astolfo. ...