We narrowed to 173 results for: GFP

This blog post explores how commonly used GFP variants can undergo Green-Red photoconversion.

Aug. 4, 2015, 2:30 p.m.

Green fluorescent protein (GFP) and its derivatives can be used experimentally as fusion tags, biosensors, reporters, and more.

May 15, 2014, 3:33 p.m.

GFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.

Aug. 23, 2018, 12:05 p.m.

Connie Cepko's lab at Harvard University has developed transcription factor and CRE recombinase variants activated by GFP. Learn more inside!

Nov. 24, 2015, 3:30 p.m.

Learn about some things to consider when designing your fusions - where to make the fusions, the size and shape of the protein, and linker size.

April 9, 2019, 1:13 p.m.

FlipGFP is a genetically encoded caspase reporter that contains a flipped beta strand of GFP. There is no fluorescence unless the caspase cuts the cleavage site between the strands.

May 21, 2019, 12:10 p.m.

Learn about the history of fluorescent proteins and download our new Fluorescent Proteins 101 ebook!

Aug. 7, 2017, 1:58 p.m.

SunTag amplifies fluorescent signals by recruiting multiple copies of GFP. SunTag can help you turn up the brightness in your fluorescent imaging experiments.

March 28, 2017, 2:30 p.m.

Tips to help you choose which fluorescent protein to use in your experiments.

May 20, 2014, 2:06 p.m.

Fluorescent biosensors can be used to measuring changes in autophagic activity over time. Several autophagy biosensors, such as Rosella, Keima, and tandem fluorescent proteins are described.

Jan. 22, 2019, 2:41 p.m.

Exciting new plasmids, viruses, and antibodies in the Addgene collection!

Jan. 31, 2023, 2:15 p.m.

Two techniques developed by Addgene depositors have made it possible to track mRNA translation at the single molecule level using fluorescent proteins.

Aug. 1, 2017, 1:15 p.m.

Use these CRISPR base editing reporters to track base editing events in real-time and enrich for base edited cells.

July 7, 2020, 1:15 p.m.

Learn about items from Addgene's viral service including lentiviral preps expressing SARS-CoV-2 proteins, ChR2 for targeted expression, DREADDs, GFP dependent Cre and FLPo, and more.

May 28, 2020, 1:15 p.m.

Find some tips and tricks for using CRISPR for gene editing.

March 7, 2017, 3:30 p.m.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Feb. 23, 2016, 3:30 p.m.

Learn how RANbodies provide a scaffold for new, easy to detect, nanobodies.

April 10, 2018, 12:56 p.m.

Learn about recently available viral vectors and viral vectors coming soon from Addgene's viral service including calcium sensors, GABA sensors, voltage sensors, and control AAV.

March 26, 2019, 1:40 p.m.

Many commonly used fluorescent proteins form dimers producing artifacts in a variety of experimental settings. Learn how to avoid these artifacts.

April 19, 2016, 2:30 p.m.

Addgene Board Member Connie Cepko gives us the scoop on progress in retinal gene therapies and the development of GFP-activatable tools in her lab.

Dec. 8, 2015, 3:30 p.m.