Skip to main content
Addgene
Showing: 121 - 140 of 243 results

  2. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene.  CRISPR-Act3.0, developed by Yiping Qi's...Shirley Liu's lab targets all mouse genes with 10 gRNAs per gene. The guides are optimized to maximize on-target...

  5. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...use CombiGEM to barcode small libraries of CRISPR gRNAs to use for screening experiments. Get the full details...modular RNA binding proteins, dCas9, and a modified gRNA to direct a variety of customizable functions to...

  6. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ... plasmid or in vitro transcribed, and changes in gRNA secondary structure can affect editing efficiency...will represent a key advantage over Cas9, for which gRNA sequence can greatly affect editing efficiency. ...

  7. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...iTOP efficiently took up both Cas9 protein and a gRNA (yes, iTOP can also be used for RNA transduction...of iTOP. iTOP can be used to transduce Cas9 and a gRNA, which subsequently enter the nucleus and cleave...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...for targeted mutagenesis of a site of interest. A gRNA is used to direct the PolI3M-nCas9 complex to a ...window also increased the likelihood that a single gRNA would introduce more than one mutation near the ...

  10. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...shRNA sequence specific to your gene’s mRNA or a sgRNA targeting an exon in your gene of interest, for ...with many applications. These libraries include sgRNAs targeting the entire genome, overexpression of ...

  11. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation... to a dead Cas9. The system is led, via a custom gRNA, to your gene of interest to temporarily increase...

  15. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed gRNA systems...

  16. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool...cerevisiae genes with 6-12 guides per gene where gRNA expression is induced by anhydrous tetracycline....

  19. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...those generated by multiplexed delivery of multiple sgRNAs. Since multiplexing is commonly used to create ...helps the researcher see which of the multiplexed sgRNA was involved in a particular edit, and which type...

  20. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in second a second...small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially all of an AAV’s ~4.5 kB packaging...
Showing: 121 - 140 of 243 results