We narrowed to 206 results for: Kars

1. Plasmids 101: The Wide World of Natural Plasmids

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   ...bacteria when we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA passengers....

2. Illuminating Epigenetics with A FRET Based Biosensor

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   ...References 1. Ong-Abdullah, Meilina, et al. "Loss of Karma transposon methylation underlies the mantled somaclonal...

3. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   ...recognized by biotin ligases normally expressed in eukaryotic cells. Using this tag reduces the number of CAPTURE...

4. Polymerase Chain Reaction Overview and Applications

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   ...time period. PCR was invented in the early 1980s by Kary B. Mullis who later shared a Nobel Prize in Chemistry...

5. Degrading DNA with Cascade-Cas3

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   ...1477. https://doi.org/10.1038/s41587-019-0310-0 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,...

6. The AAVantages of AAV in CRISPR Screens

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   ...get around this restriction. The most obvious workaround is to use a multi-plasmid system, where you have...

7. Transferable Skills Guide: Managing a Team

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   ... Karen Guerin...

8. Plasmids 101: Knockout/Knock-In Plasmids

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   ...Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "Overview: Generation of Gene Knockout...

9. Light Sheet Fluorescence Microscopy

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   ...tetrahydrofuran-based tissue clearing (13,14). A few years later, Karl Deisseroth’s lab developed CLARITY, which is a hydrogel-based...

10. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    ...we have right now are well adapted to work in prokaryotic bacteria, not in fungal systems or plants. We...

11. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    ...and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed Boyden crop up in this book was...

12. Isolating Genomic Regions of Interest with the CRISPR System

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    ...modular RNA-guided regulation of transcription in eukaryotes." Cell 154.2 (2013): 442-451. PubMed PMID: 23849981...

13. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...protein expression that occur in cells where common eukaryotic promoters (such as CMV or EF1A promoters found...

14. AAVs in Retinal Gene Therapy

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    ... Karen Guerin...

15. An Introduction to Adenovirus

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    ...and resources References Bauzon, M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). ...

16. Getting to Know Expansion Microscopy

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    ...10.1038/s41592-018-0238-1  Klimas A, Gallagher BR, Wijesekara P, et al (2023) Magnify is a universal molecular...

17. Course-Based Undergraduate Research Experiences (CUREs)

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    ...When Karl Barry Sharpless was awarded this year’s Nobel Prize in Chemistry, he joined an elite club, ...

18. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    ...References 1. Buchholz, Ursula J., Stefan Finke, and Karl-Klaus Conzelmann. "Generation of bovine respiratory...

19. Cpf1: A New Tool for CRISPR Genome Editing

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    ...Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary classification...

20. Plasmids 101: Plasmid Incompatibility

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    ...(akin to sister chromatids coming together in eukaryotic cells). The partition NTPase is recruited and...