We narrowed to 286 results for: LUM
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TypeBlog Post...qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini et al., 2012) measures...homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls. This assay...
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Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...primer to each template. The PCR product is then column purified. Degrade wild-type template strand: Next...23766324. PubMed Central PMCID: PMC4409680. 2. Mccullum, E. O., Williams, B. A., Zhang, J., & Chaput, ... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post...sensitivity, and photostability under two-photon illumination than previous GEVIs. Some constructs are also...00916-3. doi: 10.1016/j.cell.2022.07.013 pYtags illuminate RTK signaling Receptor tyrosine kinases (RTKs... -
Plasmids 101: Dimers and Multimers
TypeBlog Post... sequencing, (which differ from the short-read Illumina MiSeq sequencing that Addgene uses.) However, ...article. Long-read sequencing Addgene uses Illumina MiSeq NGS to perform whole plasmid sequencing ... -
27 Hot Plasmids from 2016
TypeBlog Post...recently developed a new method called DULIP (dual luminescence-based co-immunoprecipitation) to overcome the... this kit are derived from the Ralser lab's own pHLUM minichromosomal vector, and besides being used for... pooled library Parasites from the apicomplexan phylum cause severe human and livestock diseases such ... -
Finding Your Science Policy Path
TypeBlog Post...so don’t be shy and reach out! Someone from your alumni community is a great place to start. What is science.... Many fellowship programs can connect you with alumni before and during your application process so you... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post...PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay for detection of extremely rare mutations... probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical Sciences 29.7 (2013... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post... humidity, sunlight exposure time, and watering volume on total crop production (though you would not ...Carlo Simulation Study. Front. Appl. Math. Stat. Volume 2 (2016). doi.org/10.3389/fams.2016.00002 ... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...(Erdmann et al., 2019). Check out our recent Illuminating Choices blog post for more discussion about ...from Addgene Better Dyeing Through Chemistry Illuminating Choices: A Guide to Selecting Fluorescent Dyes... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post...visualize the protein via fluorescence or chemiluminescence, precipitate the protein out of solution, ...antibody allows the protein to be detected by chemiluminescence or fluorescence. Western blots are frequently... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection... pyogenes Yeo pKS diaCas9_sgRNA 74923 Other/Phaeodactylum tricornutum U6 yes, cut S. pyogenes Winge pJJR50... -
Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab
TypeBlog Post...great for teachers looking to supplement their curriculum. From the details about personal protective equipment... -
New FLIS Plasmid (April Fools 2022)
TypeBlog Post...Light Interval System, or FLIS, incorporates a bioluminescence protein into the chitin that can be activated... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog Post...from lab gloves. The tree was adorned with a bioluminescent tree topper, tube “lights,” and autoclave tape... -
The Codon Guide to Synthetic Biology
TypeBlog Post... Press and is designing a genetic engineering curriculum at MIT. He also writes Codon, a blog about biotechnology... -
Twelve Amazing Guest Blog Contributors!
TypeBlog Post... to selecting fluorescent dyes and ligands. Illuminating Choices Paul Heisig, who shared his flow cytometry... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog Post...Challenge, Westenberg’s course includes Winogradsky columns and characterizing the microbes from the termite... -
More Data for You: Find Articles Citing Addgene Plasmids
TypeBlog Post...the "Resource Information" heading in the right column of the plasmid page. Check out the purple arrow... -
Scientist Networking: What is an Informational Interview?
TypeBlog Post...for interesting connections is your university’s alumni database. Sharing a school is a little connection... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post...clicking on the links in the "Agree" and "Disagree" columns found in the Reagent Resources table or by downloading...