...fluorophore--think of it as a virtual photon 3. Relaxation of the acceptor fluorophore back to its ground...donor-acceptor pair and a change in FRET efficiency correlates with a change in the distance and/or orientation...simultaneously would further increase the amount of correlated information available from a cellular process...

... HR carefully because sometimes companies have relaxed dress codes for interviews. You can also ask HR... know up front how you plan to take questions.  Relax!  You wouldn’t have made it to the interview if ...position. So prepare for the interview, but then relax.  Additional resources on the Addgene blog: Tips...

...identical antibodies (See Fig 1.). rAbs are a relatively new technology that takes advantage of DNA sequencing... NOT align with the atlases/literature and is unrelated to the target protein, we categorize the antibody...strong signal. However, exposure time and its relationship to signal depend on what you’re comparing your...

...   Epigenetics nation stuck with methylation correlation  In the 1980s, a lot of things were cool: Walkmans...problem is that many of these studies report correlations without clearly testing whether a certain methylation... dCas9-TET, allowing one to study the causal relationship between DNA demethylation and gene expression...

...embark on or continue their careers in science-related fields. In particular, two recent events have the...note is a ruling in August by the National Labor Relations Board to allow graduate students who work as teaching...university and alterations to the student-university relationship, along with, of course, union dues. Time will...

...restriction enzyme and ligase based cloning is a relatively inexpensive way to get the job done, provided...because the T4 polymerase needed for this method is relatively inexpensive and can be obtained for about $70... or purify DNA fragments from gels. If you’re relatively new to the cloning game, however, or simply looking...

...protein, it's important to validate its function relative to the untagged, wild-type protein. You may, for...the protein of interest and then analyzing the relative colocalization of their fluorescent signals and...One should also be aware of additional problems related to the properties of FPs themselves. These can ...

...of protein concentration. Calibration curves correlating this ratio to the total expression time can therefore... situation where one wants to understand the relationship between the age of a cell, protein, or cellular...nascent tissues and the formation of limbs are correlated with changes in gene expression. In a developing...

...cases arise and millions of people die from cancer-related complications each year. Despite these grim statistics...Ras researchers, one of the most exciting and relatively new resources is the RAS Pathway Clone Collection... initiative to curate our collection of cancer-related plasmids such that each is linked to a known major...

...they want to understand the ultrastructural relationships that influence everything from cell division...physically separates molecules while maintaining their relative positions, allowing you to resolve individual ...accessible imaging equipment, ExM protocols utilize relatively inexpensive reagents and are readily adoptable...

...employees. The teambuilding culture helps us build relationships and thereby work better together.I realize I...encourage everyone to make an effort to build relationships with their colleagues. It can be easy to get...done everything from kayaking, to field day, to relay triathlons, to 5Ks. Many of these examples we’ve...

... Thus far Plasmids 101 has covered GFP and its related fluorescent proteins, which are sometimes used ...crystallizing it or testing its function. Small ubiquitin-related modifier (SUMO) can help with folding and stabilization...and purification An affinity tag, generally a relatively small sequence of amino acids, is basically a...

...and bacterial transformation. And more plasmid-related educational resources can be found in our Plasmids...creating and editing Wikipedia pages, OWW will be relatively easy for you to begin using. For the novice, ...the repository. These protocols show up on the related plasmid pages and can help scientists use these...

...common fluorescent proteins and their mutations relative to wildtype GFP. Although not listed here, many...spectrum are not GFP derivatives, but are instead related to the dsRed protein isolated from Discosoma sp... fluorophores Fluorescent Protein Mutations Relative to Wildtype GFP EGFP F64L; S65T EYFP S65G;...

...the ratio of genome-containing viral particles relative to the total number of viral capsids (which can...vectors consist of full particles (white arrowhead) relative to empty particles (black arrowhead). Scale bar...absolute titer values may not be as important as the relative titer values. Finally, because physical titer ...

... added on a regular basis, so the ecosystem of related tools is always growing. Beyond the ability to ...understand and/or attempt to reproduce research relating to data deposited into a repository access to ...can be viewed – and conceivably additional work relating to reproducing the original research can be documented...

...basis of shuttle vectors, which can function in unrelated host species, even from completely different kingdoms...replication machinery. A shuttle vector designed for unrelated bacteria should include oris for both target species... lab tools, and they include both vectors for unrelated bacterial species and those that can shuttle between...

... expand as Rett scientists deposit their MECP2-related plasmid tools with Addgene and share additional...Methyl-CpG-binding protein 2 mutations and phenotype-genotype correlations. Am J Med Genet 97:147–152 . https://doi.org...Determines the Clinical Course of Rett Syndrome and Related Disorders. Cell 152:984–996 . https://doi.org/10.1016...

...Also, in cases where the protein of interest is relatively small, adding GFP may alter diffusion rates. ...Although bioluminescent probes are usually dim relative to fluorescent probes, recent engineering efforts... that the N- and C-terminus of GFP itself are relatively close, thereby minimizing the disruption of the...

...can do and, in this way, science has always been related to government and, consequently, politics. More...Inclusion of data informing policy specifically related to the following subjects: Climate change Vaccines...Healthcare Science education To make scientists relatable (17, 20). This is an effort to show the general...