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We narrowed to 601 results for: Ret;

Showing: 121 - 140 of 601 results

  2. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...increased yields in adenoviral (Coroadinha, 2006) and retroviral production systems (Shen, 2011). To our knowledge...also improved yields of the AAV2 derivative, rAAV2-retro, but had no effect on AAV5 yields. We further show...Effect of osmotic pressure on the production of retroviral vectors: Enhancement in vector stability." Biotechnology...

  3. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...translated into proteins, or, in the case of retroviruses, reverse transcribe their gRNA into DNA, which...replicative ability. The remaining particle, which retains the protein coat (and envelope), has a “gutted”...they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And now that you know what viral...

  4. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...selected to perform the transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired...for the transduction step. Viral particles are secreted into the cellular media, making viral collection...integrate into the host genome (lentivirus and retrovirus) while others are non-integrating (adenovirus...

  5. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ... that a longer vent time would be sufficient to return the sample back to the original pH. What about...it back in the freezer? We then tested whether returning the tubes to the -80°C freezer after storage on...the pH changes in our solutions are transient and return to normal once the tube is opened and equilibrium...

  6. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...book! Bonus, Saini's following book Superior: The Return of Race Science is a terrifying and equally good...conclusion? In this series, there is always another secret."     - Marcy Patrick, Associate Director of Quality...force them on everyone I know. I am, of course, pretty biased as the founder, but our book club has become...

  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...protein secretion, making harvesting recombinant proteins a cinch. This is because it doesn’t secrete its ...proteins, making the recombinant protein its major secretion. Beyond sheer protein production, K. phaffii is...

  8. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...team members have opportunities to develop and stretch then they will be happier and more productive in...the person doing the task will have to learn and stretch a little, but be careful not to go too far. Is...Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development...

  9. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...AAV Control AAV allow researchers to not only interpret their results, but also confirm expression in ...also offered as AAV1 (along with AAV PHP.eB, AAV Retrograde, AAV2, AAV5, AAV8, and AAV9). Once activated,...Serotype Name Depositor 69570 AAV1 pAAV-EF1a-N-CretrcintG Connie Cepko 69571 AAV1 pAAV-EF1a-C-CreintG...

  10. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...transfection efficiency when delivering these viruses via retro-orbital vs. tail vein systemic injections? A: We...difference in AAV transduction efficiency between retro-orbital versus tail vein injections.   Q10: Have...successfully systemically transduce and then retransduce a model organism such as mice, e.g. first at ...

  11. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...with what works.  To balance this out, however, is pretty much every other assay you can do. Most non-HRP...Brightness Chart. Boston University Flow Core. Retrieved January 31, 2022, from https://www.bu.edu/flow-cytometry...overlap. Syracuse University Flow Core Facility. Retrieved January 31, 2022, from https://flowcore.syr.edu...

  12. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ... parts of the brain stimulated by stories. In retrospect, I should have expected this given that the brain... lead to altered function or maybe your favorite FRET-based biosensing technique can be applied to an ... at Addgene.org Addgene's Viral Service Find Retrograde AAV Browse Optogenetics Plasmids ...

  13. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...different stretches of DNA: Dam methylase adds a methyl group to the adenine of GATC stretches of DNA Dcm...you results that would either be difficult to interpret or lead you to conclude your plasmid is not correct...

  14. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...dimerization mTagBFP2-pBAD - Bacterial Expression Return to top Cyan Protein Excitation (nm) Emission (nm...Mammalian Expression MiCy-pBAD - Bacterial Expression Return to top Green Protein Excitation (nm) Emission (...mNeonGreen-C Cloning Vector - Mammalian Expression Return to top Yellow Protein Excitation (nm) Emission ...Bacterial Expression mCyRFP1-C1 - Mammalian Expression Return to top Orange Protein Excitation (nm) Emission ...Expression tdTomato-pBAD - Bacterial Expression Return to top Red Protein Excitation (nm) Emission (nm...- Mammalian Expression pMXs-mRFP1 - Mammalian Retroviral Expression mCherry 587 610 16 4.5 15 min Monomer...Mammalian Expression mCherry2-C1 - Mammalian Expression Return to top Far-Red Protein Excitation (nm) Emission...

  15. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...editing efficiencies in the mouse brain, liver, retina, heart, and skeletal muscle. The dosing required...The Ace-mScarlet voltage indicator works like many FRET-opsin GEVIs. With an increase in the neuron’s membrane...quenches a proportion of mScarlet's emission via FRET. Image from Beck and Gong, 2019. Broadening ...

  16. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...promoters from subretinal injections of neonatal mouse eyes at two different ages." Retinal Degenerative...Cepko’s Lab investigating neuronal circuits of the retina using viral tools.   References and Resources References...

  17. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...afforded by gRNA binding at non-target sites would theoretically decrease off-target cutting. Figure 1...kidney (HEK) cells, 11 of these initial mutants retained the on-target activity of wt Cas9 while decreasing...their work, Chen et al. 2017 used single molecule FRET measurements to determine which portions of Cas9...

  18. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...spontaneously religate, this transient construct retains functional Type IIS sites and will be re-digested...is irreversible because this construct does not retain the enzyme recognition sites. As a result, the ...design of flanking overhangs. Although there are theoretically 256 distinct flanking sequences, sequences that...

  19. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...and there is a greater burden on the reader to interpret the results to see if it leads you to the same...they mean. Could there be any other possible interpretation of the results? Then think deeper about the...doesn’t mean that there are no mistakes or misinterpretations. After all, the science was performed, written...

  20. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...cells are then grown in biotin-containing media. FRET is another example of a protein fragmentation complementation...used to study binary protein interactions. However, FRET generally only tells you if two proteins interact...Find Plasmids from the Roux Lab Find Plasmids for FRET ...
Showing: 121 - 140 of 601 results