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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 399 results for: SEF

Showing: 121 - 140 of 399 results

  1. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...both a stable haploid and diploid state which is useful for genetic analysis, as well as an efficient mechanism...shuttling between E. coli and yeast cells. To be useful in the lab, the vectors must contain a yeast-specific...

  2. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...the so-called ‘constant’ region which is, quite usefully, both conserved across and unique to a species...applications. Assay use While secondary antibodies are useful signal amplifiers, the flexibility of their use...

  3. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...your protein. For the same reason, they’re also useful in experiments where the availability of some regions...sensitivity, polyclonal antibodies are particularly useful for certain research applications. Many secondary...

  4. Addgene: The First Twenty Years

    Type
    Blog Post
    ...don’t get nominated for it, it just reflects how useful the reagents we created are for the community. ... once more. Our ready-to-use viral vectors were useful for researchers wanting high quality reagents, ...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    ...your refrigerator often need a little help to be useful. Mainly because, well, antibodies are kind of hard...2007; Stoeckius, et al., 2017). Metal isotopes are useful for mass cytometry (Bendall, et al., 2012). And...

  6. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...key part of being a scientist and an incredibly useful transferable skill. Being able to stand up in front...’ve developed a valuable skill set that will be useful in nearly any other job you may choose to pursue...

  10. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...easily visualized.  The tool-kit consists of highly useful vectors compatible with Gibson and Saptrap cloning... includes an array of FPs as well as additional useful tags, including 3xFLAG epitopes, TEV and BioTag...

  13. Lab Automation at Addgene

    Type
    Blog Post
    ...it easy to spread out your samples. This can be useful if you’re transferring samples from 96 wells plates...machines I have used in the lab. It’s especially useful if you want to avoid sore fingers. There are other...

  14. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...a gene of interest. These plasmids will be most useful for constructing intermolecular FRET probes, where...acceptor fluorophore on the same protein and are useful when a process affects the conformation of the ...

  15. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...as clinical therapy. These biological tools are useful because they’re derived from viruses that can infect...0.5% peracetic acid, 10% bleach, or 1% iodine are useful. Some organizations or cities may require a higher...

  16. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...cloning, sequencing, and genotyping. It’s extremely useful but is not as quantitative as other methods of ...primers all in one PCR reaction. This is an extremely useful PCR method that can help save time and effort in...

  17. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...inducible promoters. Inducible promoters are extremely useful if a desired protein product is toxic to cells ... with labelled or modified amino acids that are useful in different downstream applications. Cell-free...

  18. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ... for improvement. These sensors still have many useful applications, though, and if you’re in the market...to the membrane, otherwise they aren’t all that useful. The cellular localization component is typically...

  19. Pooled Library Amplifications

    Type
    Blog Post
    ... easier to work with. In either case, it can be useful to run a restriction digest of your amplified library...general pDNA Library Amplification protocol are useful references. Skim through our Pooled Library page...

  20. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is both enduring and ...community?  Addgene exists to help researchers share useful tools, and as such, we’re always looking to understand...
Showing: 121 - 140 of 399 results