We narrowed to 181 results for: SPEN;

1. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   ...but risking the potential of them not working, or spend a few weeks designing and cloning the vectors you...

2. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   ...with one of these. After all, there’s no sense in spending valuable research dollars on a new secondary antibody...

3. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   ... your application? Like many scientists, I have spent hours sifting through comments and papers to find...

4. Lab Automation at Addgene

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   Blog Post

   ...the volume of the smaller aliquots you’d like to dispense and you’re on your way. Label your tubes with...

5. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

   Type

   Blog Post

   ...and how they work to overcome them, and why she spends some of her time engaging with others on Twitter...

6. Common Injection Routes in Mice

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   Blog Post

   ...injections are most often used when injecting a cell suspension to create xenograft models. Restraint: back scruffing...

7. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

   Type

   Blog Post

   ... we started, too much time for this project was spent selecting guides, designing primers, and running...

8. Academic vs. Industry Postdocs

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   Blog Post

   ...explore scientifically since you can’t afford to spend too much time working on something extremely risky...

9. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   ...solutions are carefully layered and then the viral suspension generated during AAV production is overlaid. ...

10. Transferable Skills: Negotiation

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    Blog Post

    ...student, I was negotiating with my PI on how to spend my grant money (Can I attend this conference?) and...

11. 10 Basic tips for mammalian cell culture

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    Blog Post

    ...achieve a consistent growth rate. For every minute spent organizing, an hour is earned (Benjamin Franklin...

12. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    ... did not lack motivation, but he didn’t want to spend hours reviewing papers to piece together the best...

13. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    ..., but with longer homology arms comes more time spent cloning. In contrast, the short homology required...

14. Plasmids 101: Protein tags

    Type

    Blog Post

    ...protein. Energy and cellular resources are being spent to make something the cell doesn’t need to make....

15. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    ...arrive from labs around the globe. The packages have spent days to weeks in transit and some have been transported...

16. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    ...study inflammation and neurodegeneration. He now spends his time learning and writing about science whenever...

17. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    ...game changing nature. Full disclosure: Zimmer also spends some time on Addgene’s role in the rapid dissemination...

18. DNA Purification Without a Kit

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    Blog Post

    ...Finally, DNA is precipitated with alcohol and resuspended in TE buffer. After this, the DNA is ready for...

19. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    ...publications in a specific field(s). Most anyone who has spent any time in academia has likely had the opportunity...

20. Negative Can Be Positive: Open AAV Data with Addgene

    Type

    Blog Post

    ...effort to test and optimize our vector - overall, I spent two years testing and reformulating our AAV construct...