We narrowed to 579 results for: SUB;

...introduced into the B. subtilis genome. Mobile-CRISPRi is then transferred from B. subtilis to the bacterium...CRISPRi into a single bacterial strain, such as a B. subtilis, or a narrow range of bacterial species, such ...integrative and conjugative elements in Bacillus subtilis (ICEBs1) system. ICEBs1 carries the genes needed...bi-parental mating. Following excision from the B. subtilis genome, the Mobile-CRISPRi ICE element forms a...integrative and conjugative element in Bacillus subtilis." Plasmid 86 (2016): 14-25. PubMed PMID: 27381852...

...please!)... Abi! Thank you so much to everyone who submitted names*, voted, and helped us on our arduous quest... antibody launch in late March!      *If you submitted the names Abi, Lin, or Blottie (our top 3 choices...joining us on our scientific adventures.  You can submit your name suggestions here or by using the hashtag...and it can’t hurt if it’s also a great pun.) Submissions are open until Feb. 3, 2022.  The Addgenies will...choose the new name! Additionally, anyone whose submission is included in the final four will receive some...

...heat block Approach: Viral RNA is extracted and subject to isothermal amplification by primers that target...cleaves the supplied fluorescently labelled ssDNA substrates. Upon cleavage, the fluorescence is no longer...bath Approach: RNA is extracted from a sample and subject to isothermal recombinase polymerase amplification...-2 in the samples. First, RNA is extracted and subject to reverse transcription. This is then amplified...Polymerase and then transcribed to provide RNA substrates for Cas13. Cas13, upon finding complementarity...between the gRNA and a given fluorescently labelled substrate, gets activated and performs non-specific collateral...

...possible to build gene regulatory RSMs (GRSMs, the subset of RSMs that regulate gene expression) that uniquely...attB-attP pairs on a register to record all permuted substrings of a set of chemicals. Specific examples of arrangements...arrangements that should be able to record all permuted substrings of up to seven chemical inputs are given in Table...population to correctly record each possible permuted substring of the inputs, and that five hour inductions were...cells to correctly record each possible permuted substring of inputs (see Figure S9 of our paper (1)). We...described in the literature (5, 6) and may be substituted for one another in an RSM. The predictability...

...activities. Working with proteins requires a substantial amount of properly folded, relatively pure protein...doing some research, notably determining the subcellular localization and the number of disulfide bonds...Tags. Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids...than E. coli, specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory.... pCri vector pCri-13a, designed for use in B. subtilis, contains the MISTIC (membrane-integrating sequence...

...updated! Subscribe to the Addgene newsletter for updates on all things Addgene; or subscribe to the Addgene...available for Packaged on Request service. A small subset of AAV plasmids will have technical or legal restrictions...of choice, pick what size prep they’d like, and submit the request. Request details can be accessed within... 100 µL (2.5 mL), or 50x 100 µL (5 mL).  Once submitted, the request will be reviewed by Addgene for legal...

...with a paired t-test, because they eliminate subject-to-subject variation (the test assesses the sum of differences...an unpaired t-test, which does account for subject-to-subject variation in its analysis (the test assesses...unpaired t-tests Samples that come from the same subject or population - before and after a treatment, for...between pairs). Samples arising from different subjects or populations (a treatment group vs. a control...

...regions. The resulting viral-infected neurons are subsequently photo-stimulated through fiberoptic cannulas...ability to specifically infect defined neuronal subpopulations, and this represents a major downside in optogenetics...electroencephalography evidences of arousal in mice previously subjected to steady-state general anesthesia (Taylor et ...ago, however, it was completely unclear which subpopulation of DA neurons could activate appetitive rather...recorded the electrical impulses of discrete subpopulations of this brain area, demonstrating that it is...and Jamie Maguire. "Characterization of a novel subtype of hippocampal interneurons that express corticotropin‐releasing...

...Kanamycin aminoglycoside Binds 30S ribosomal subunit; causes mis-translation Bactericidal 50-100 ug/...Spectinomycin aminoglycoside Binds 30S ribosomal subunit; interrupts protein synthesis Bactericidal 7.5... Erythromycin macrolide Blocks 50S ribosomal subunit; inhibits aminoacyl translocation Bacteriostatic... Tetracycline tetracyclin Binds 30S ribosomal subunit; inhibits protein synthesis (elongation step) ... ug/mL Chloramphenicol   Binds 50S ribosomal subunit; inhibits peptidyl translocation Bacteriostatic...

...database design (schema).  Registered users can submit new fluorescent proteins, add data to existing ...database is free from the risk of inaccurate data submissions, so FPbase includes a versioning system that ...and to view previous versions.  Currently, all submissions also require a DOI referencing primary literature...published literature as soon as possible after submission. Using FPbase The most obvious use case for FPbase...experience in Python, fork the project on github and submit a pull request. Many thanks to our guest blogger...

... own subclone, they were able to develop a 12-plex multiplex imaging system. Mixing the subclones within...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... a fluorescent protein coupled to a specific subcellular localization peptide. By combining three different...their work with the “signalome,” a 12-plex mixed-subclone system using mStrawberry, localization peptides...

...Monitor neo-substrates Small molecule degraders may recruit CRBN to nonstandard or “neo-substrates.” We advise...evaluation of degradation of known cereblon neo-substrates (IKZF1, IKZF3, GSPT1, ZFP91, and CK1a). While...While expression of these cereblon neo-substrates varies in different contexts (for example, IZKF1 and IZKF3... to date, we have not observed off-target neo-substrate degradation with dTAG-13. Example experiments ...

...spaced CRISPR software submissions. With few prior publications to go on, a submitted manuscript could only...few moments after the piñata burst, value was subjectively ascribed relative to the basic needs of each...software design that is applicable to only one, or a subset of organisms. In only a few cases the software ... agnostic. Most tools are exclusive to one or a subset of organisms. Examples: “organism agnostic”, “model... you can rule out any tool that limits you to a subset of specific genomes. These are most of the tools...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...distribution of clusters without accumulation in subcellular compartments such as nuclear speckles. Furthermore..., by fusing a fragment of the kainate receptor subunit to the C-terminus of the protein. Unlike the diffuse...construct in which TagRFP-T and Dronpa flanked a PKA substrate fused to the phosphopeptide binding domain FHA.... The FHA1 domain binds to phosphorylated PKA substrate, inducing a conformational change that placesTagRFP-T...

...Did you know that one of the quickest ways to submit deposits with more than 10 plasmids is to use our...the page, you’ll see two options for data entry: “Submit Online Deposit” and “Download Deposit Spreadsheet...Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet...Plasmid sequences or GenBank files. We encourage submission of QUEEN-generated GenBank files (Mori and Yachie...Download a new spreadsheet each time you want to submit new plasmids! We update the spreadsheet every so...

...they are looking for in grant submissions and will direct you on how to submit your work either through seminars...writing and hopefully increase your success rate. 1. Submit your grant early! First off, and perhaps the most...for you to make the relevant alterations before submitting - handing them your application 3 days before...their scoring system or meeting with their grant submission panel. Some funding bodies provide opportunities...

...The blood-brain-barrier (BBB) exists to prevent substances in the blood, like proteins and viruses, from...mechanism unknown)   Chen et al., 2023 AAV9.452sub.LUNG1 Lung: ATII cells Mice N/A Goertsen... encompasses more molecularly-defined neuronal subtypes and with less bias among them than AAV-PHP.eB ...type II cell transduction in the rodent lung AAV9.452sub.LUNG1 is an engineered capsid derived from AAV9 ... does express in the brain. Both AAV9 and AAV9.452sub.LUNG1 have significantly higher off-target expression... validation, validation! (psst… don't forget to submit your validation data to our Data Hub once you’re...2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(9), 1449–1464.e13...

...After production, all virus preps are titered and subjected to quality control by Addgene before being distributed...VP2, and VP3. Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed...Silver staining of purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins ...lentiviral preparations (described above) that are subject to a concentration step prior to being frozen. ...gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for normalization purposes...

...chemiluminescent substrates are available. Labs with limited equipment often opt for the chromogenic substrates since...For enzymatic signal detection, users provide a substrate that the enzyme converts into a detectable product...antibody. The reporter reacts with a provided substrate and produces a signal that is directly proportional...enzymatic reaction between the detection antibody and substrate is proportional to the amount of antigen present...

...variety of topics. Individual forums are hosted on subreddits (r/”topic”) which are essentially lists of forums...Software for Biology) on Reddit and got some substantial traffic that we thought it would be a good idea...engaged, and has great questions! 2. The r/Science subreddit is well moderated. One of our original worries..., and even coached us on how to write our AMA submission to avoid rousing the trolls. 3. The AMA process...organized. You can check out the r/Science AMA Submission Guidelines here, but essentially we just needed...