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We narrowed to 447 results for: SUP

Showing: 121 - 140 of 447 results

  1. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ...planning meetings with each trainee to create a supportive, growth-oriented lab culture. The aim of these...summarized briefly below (and available of in the supplement of this paper), help each person organize his...Strategies to Resolve Conflict With Difficult Supervisors Management for Scientists: Seeking Feedback Management...

  2. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...metastasis CRISPR screen. The Chen Mouse Tumor Suppressor Gene CRISPR Knockout Library is an AAV CRISPR...CRISPR knockout library targeting mouse tumor suppressor genes (TSG). Find the Mouse Metastasis CRISPR-Cpf1...Double Knockout Library Find the Mouse Tumor Suppressor Gene CRISPR Knockout Library If you have a new...

  3. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ... is resistance to change, public support is weak, government support is “feckless”.  So what can be done...from Harvard Medical School and a long list of supporters from all of the research centers in Boston.  ...

  4. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...results section, but you had to dig through the supplemental files to see the actual data. Now you’re wondering...datasets, which are often obscured in large supplementary data files, BioGRID ORCS makes it possible for...sgRNA-level sequence read scores. The original supplemental data tables available with the publication are...

  6. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...research area is not as important as choosing a supportive, positive role model. By contrast, as a postdoc...is crucial that you choose an advisor that will support your growth in your science career. Will you be...thinking about a non-academic path, will the PI support this path and help you pursue any career alternative...

  7. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...Candidate, University of Ibadan, Nigeriaunder supervision of Dr. Akindele Oluwatosin Adeyi, University ...Otito Izu is a PhD Candidate working under the supervision of Akindele Oluwatosin Adeyi at the University...the user.   At Addgene we're happy to be able to support diverse groups of scientists working in a wide ...

  8. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...during the pandemic and in the future, depends on support from the scientific community. Deposits of new ... be deposited into repositories, and financial support to maintain repositories’ capacity for storing,...all ways in which the scientific community can support repositories. Repositories are essential for the...

  9. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ... 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of gene editing...Kim et al 2014) have shown that this technique supports extremely high frequencies of genome cutting and...nucleofection as (in our hands) this technique supports gene disruption or gene replacement frequencies...

  10. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...Gentamicin is often used as a supplement in mammalian cell culture to suppress bacterial growth, and is not...like gentamicin, neomycin is typically used to suppress bacterial growth. The selection agent should not...

  11. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression...Fishell   Optogenetics Optogenetics continues to supply neuroscience with ever more efficient tools for...

  12. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...Renilla luciferase variant fused to an FP; when supplied with a luciferase substrate, the luciferase transfers... a luciferase substrate like coelenterazine is supplied in the culture medium to allow RLuc8 to produce...coelenterazine-h to coelenterazine-h, producing a constant supply of substrate. Using this compound to excite the...

  13. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...COVID-19 pandemic, students will face a reduced supply of internships as a result of the necessary restrictions...the request to help your recommenders deliver a superb and detailed portrayal of yourself. Be mindful ...likelier you will end up with a stellar letter of support.        5. Application submission  Set a calendar...

  14. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...aspects of my work but also found fulfilment in supporting my team’s development and career growth. This...check-ins, which helped them stay on track and feel supported. Another team member was highly self-motivated... served as a great "baby project," offering a supportive environment to build my management skills. Finally...

  15. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...adenovirus genes – E4, E2a, and VA – which are supplied on a helper plasmid and mediate efficient viral...including the ones listed previously). E3/E1B suppresses both apoptosis and the host immune response to...making it replication deficient. This is typically supplied by the production cell line (HEK-293) during production...

  16. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...openUC2, which I am a co-founder of), that aim to support the community in developing better, more sustainable...Challenges such as the provision of an accessible supply chain for parts that are listed in the bill of ...-based models and the use of taxpayer money to support this research. But there is a move towards more...

  17. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...save yourself a headache later on. Enhancer and suppressor screens These types of screens are very similar...identify manipulations which either enhance or suppress the previously defined disease phenotype. These... a mutant phenotype, very similar to enhancer/suppressor screens. Selections Selections differ from screens...

  19. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...was particularly helpful for securing funding to support this effort. The future of reproducibility workshops...Science mini-grant, which will fund a project to support a dedicated website to host all the materials in...collaboration is that it is driven by academics, with support of industry partners, not the other way around....

  20. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...genes with 6 gRNAs per gene. The second is a "supplementary" library that contains an additional 6 gRNAs...expression of the MutL mutant enables transient suppression of DNA repair during oligonucleotide integration...Zurovec pBlue-TAL Nathan Lawson pJDS Series   Superglue proteins with SpyTag/SpyCatcher and SnoopTag/SnoopCatcher...connectors” that link tags to the targeted gene are supplied to the reaction from a prebuilt donor plasmid ...sequences for homology arms and the guide RNA are supplied as annealed synthetic oligos - eliminating the...
Showing: 121 - 140 of 447 results