We narrowed to 1,073 results for: TOR

.... We’re here to help with that! Four important factors to consider for any experiment are: Speed Cost...and colleagues. Here you simply PCR-amplify your vector and your insert, DpnI digest away the templates... time is of the essence. This method requires a vector backbone containing a topoisomerase I cut site,...the type IIS enzymes in the reaction to redigest vector reseals and other incorrect products. This feature...use of toxic ccdB to prevent any non-recombinant vectors from propagating. The ligation-independent methods...Addgene.org0 Check out protocols for plasmid cloning Find vector backbones Read our molecular biology reference ...

...or lentiviral vectors in our previous blog posts.  Viral vector injection Viral vectors allow a tight ...neuronal function directly such as receptor subunits or neurotrophic factors. Another option uses optogentics...is cloned into a viral shRNA expression vector and the vector particles are delivered into the brain region.... On the other hand, lentiviral vectors are superior packaging vectors for larger genes - they can hold...around 1012-1013 vg/ml (vector genomes per milliliter) and lentiviral vectors around 108 IU/ml (infectious...using viral vectors By modulating neuronal gene expression and activity, you can use viral vectors to study...viral vectors blog posts Find tips for preparing AAVs View our Beginner's Guide to Viral Vectors Resources...

... taking up a position on the Addgene Board of Directors. We're excited to watch both Addgene and Sample...How to Be A Good Manager from Addgene Executive Director Joanne Kamens Resources on the Addgene Website... Check Out the Other Members of our Board of Directors Become a Part of the Addgene Community ...

... a Good Mentor? Choosing a Good Mentor for Scientists Will You Be My Mentor?   I Have a Mentor: What now...your own peer mentoring circle. In a Peer Mentoring Group all participants act as both mentors and mentees...Addgene Blog Mentoring for Scientists Series. I have been thinking a lot about Mentoring for over 10 years...mentees. So I experimented with a group mentoring format where 1 mentor met with a group of mentees to get ...her mentoring style. While working on this project, I read the book “Every Other Thursday: Stories and...success. Their stories made it clear to me that, executed correctly, the group mentoring format could work...Science (AWIS) mentoring programs. The groups in these programs typically consist of 1-2 mentors and 3-5 mentees...

...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in...transferred in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays...in-frame to these vectors. Plasmid... Plasmid Collections Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger... zebrafish) pDW1775 (for expression in plants) Vectors encoding heterodimeric FokI nuclease domains: pMLM290...

...transcription factors to increase gene expression. CRISPRa methods vary in their transcriptional activators: some...potent transcriptional activators (p65 and Rta). These transcriptional activators work in tandem to recruit...generally thought of as the “first generation” CRISPR activator. While it requires a relatively simple construct...modest gene activation. Synergistic Activation Mediator (SAM) Description SAM uses specially engineered...levels of gene activation compared to other CRISPR activators, making it a popular method for gene activation... SunTag performs better than first generation activators while showing lower activation levels than SAM...transcription. This complex consists of the VP64 activator used in other CRISPR activation methods, as well...

...adenine base editors with improved editing in cell lines Nicole Gaudelli, the Associate Director and Head ...Adenine base editors (ABE) mediate A•T-to-G•C base changes (Figure 1), but it can be challenging to make...to create the eighth generation of adenine base editors, called ABE8 (Gaudelli et al., 2020). The generated...to increase the targeting scope of adenine base editors The most commonly used S. pyogenes Cas9 requires...targeting scope for base editing.  Try ABE8 base editors for your research! Base editing in primary human...human cells  Cell therapy approaches can use base editors to correct mutations in stem cells and return the...Reducing off-target editing in ABE8s While base editors are precise molecular tools, they do have undesired...

...A newly established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering...systematically established way of making an all-in-one vector for multiplex genome engineering.” Now, they’...ease and efficiency for all-in-one CRISPR/Cas9 vectors expressing up to seven guide RNAs (gRNAs). Sakuma...Schematic overview of the all-in-one CRISPR/Cas9 vector construction system for multiplex genome engineering...expression cassettes of the gRNAs into a single vector in tandem. They went on to show that their CRISPR...CRISPR/Cas9-nuclease and CRISPR/Cas9-nickase vectors work successfully to target multiple sites in cultured...it has not yet been demonstrated, they say the vectors are also likely to work in animal embryos based...

...increasing CRISPR editing efficiency, new CRISPR base editors, DIY DNA ladders. You can find additional hot plasmids... Ouabain Selection  Hot Plasmid 3: CRISPR base editors with enhanced range and specificity. Listen to ...to to the Segmennt of Improved CRISPR Base Editors Hot Plasmid 4: pPSU Plasmids for generating DNA molecular...Out All of Our Hot Plasmids Articles Browse the Repository  ...

...unlikely receptor for PHP.B, and how the Deverman and Wilson labs determined LY6A was a receptor used by....B uses the LY6A receptor to gain access to the CNS. The bottom line of viral vector permissiveness While...pathogens to the CNS, but this also stops viral vectors, such as adeno-associated virus (AAV), from reaching...injection into the bloodstream, but requires a high vector dose and is unable to reach all regions of the ...identified lymphocyte antigen 6 complex (LY6A) as a receptor for PHP.B. Read on to learn how the design of ...also known as stem cell antigen-1 (SCA-1), is the receptor used by PHP.B to reach the CNS; yet LY6A is an...Wilson Labs independently identified LY6A as a receptor for PHP.B by looking for a unique sequence of ...

...voltage indicator Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast... genetically encoded voltage indicators. To expand the voltage indicator toolbox, Ed Boyden’s lab developed... cloned into an entry vector. The 2in1 cloning system contains two entry vectors (pUC57-L1L4 and pUC57...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...name their most potent Acr-LOV fusion protein inhibitor CASANOVA for “CRISPR-Cas9 activity switching via...CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires no additional...coordinate emotions, thoughts, and behavior. Historically, scientists study the electrical activity in...

...TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning vector. The vector is 8901 base...-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning Vector A.1 The ...use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the...TRC-cloning vector. Addgene Plasmid Description pLKO.1 – TRC control Negative control vector containing... Tools page. Several other laboratories have deposited pLKO derived vectors that may also be useful for...TRC Cloning Vector The following steps were the prevailing guidelines when the pLKO.1 vector was created...Digest pLKO.1 TRC-cloning vector with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep DNA...

...B978-0-409-90042-2.50021-0  Toh, W. K., Teo, Y. L., Tor, X. Y., Loh, P. C., & Wong, H. L. (2023). Development...Cloning Vectors for Gram-Negative Bacteria. In R. L. Rodriguez & D. T. Denhardt (Eds.), Vectors (pp. 287... inform us on how to create better recombinant vectors. In this blog post, we’ll define plasmid host range...features to create broad host range recombinant vectors.  A plasmid’s host range is determined by the ...groups (Jain and Srivastava 2013).  Recombinant vectors that are made from broad host range plasmids are...different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number...The plasmid has its own replication initiation factors and proteins independent of the host. Host range...

...Guides Viral Vectors Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral...lentiviral vectors that can be safely used in laboratory settings. These engineered lentiviral vectors have ...Viral Vectors 101 topics More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...gamma-retroviruses, see Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically...the host genome. To produce lentiviral vectors in the laboratory, the non-required components have been...the supernatant containing the viral vectors is removed and stored or centrifuged to concentrate. You can...

...Science Guides Viral Vectors Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral...gamma-retroviral vectors is essentially the same as lentiviral vectors. Many gamma-retroviral vectors have selectable...of Viruses Viral Vector Elements More Viral Vectors 101 topics More Retroviral Vector topics Addgene protocols...Gamma-Retroviral Vectors Vector Production Integration Pseudotyping Common Uses Safety Resources Plasmid ...engineered into gamma-retroviral vectors that can be safely used in laboratory settings. These engineered gamma-retroviral...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...Gamma-retroviral vectors do not have defined “generations” of plasmids, as lentiviral vectors do. For more...

...receptor C MGC129539, NR1F3, RORG, RZR-GAMMA, RZRG, TOR RXFP1 relaxin/insulin-like family peptide receptor...IREL RFX5 regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory factor X-associated...cytokine receptor-like factor 1 CISS, CISS1, CLF, CLF-1, NR6 CRLF2 cytokine receptor-like factor 2 CRL2,...cytokine receptor-like factor 3 CREME9, CYTOR4, FRWS, MGC20661 CSF1 colony stimulating factor 1 (macrophage...growth factor receptor 3 ACH, CD333, CEK2, HSFGFR3EX, JTK4 FGFR4 fibroblast growth factor receptor 4 CD334...plasminogen activator, urokinase ATF, UPA, URK, u-PA PLAUR plasminogen activator, urokinase receptor CD87, ...CNTF ciliary neurotrophic factor HCNTF CNTFR ciliary neurotrophic factor receptor MGC1774 CORT cortistatin...

...and green GPCR-based NE indicators Sensitive multicolor indicators for monitoring norepinephrine in vivo...Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium Indicators. Janelia...calcium indicators for optical imaging (GECO1, GECO1.2), including green, blue, and red indicators An Expanded...fluorescent calcium ion indicator OCaMP A sensitive orange fluorescent calcium ion indicator for imaging neural...encoded calcium indicators. J Biol Chem. 2019 Mar 15;294(11):3934-3946. Katalin Torok Calcium Red Calcium...Calcium Near-infrared indicator iGECI A near-infrared genetically encoded calcium indicator for in vivo imaging...calcium indicator for fluorescence using HaloTag ligands A modular chemigenetic calcium indicator enables...

...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab ...expression vector with N-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF ...Gateway destination vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal...-1-3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc...His-tagged versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent...35S-eGFP-nosT - Transient expression vector for eGFP in plants Find FP tagging vectors in budding yeast pHT101-mCherry...

...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or search...transcription factors to further minimize background expression. Improved expression of the transactivator proteins...should also choose between: Dual vector system: express the transactivator and the tet-controlled gene of...both the transactivator and the tet-controlled gene of interest from one plasmid. A single vector approach...generations of transactivators and promoters are generally cross-compatible. Your choice of transactivator, promoter...Request service available for many transactivator or tet-inducible vectors. Tetracycline-inducible Backbones...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...

... the E2 regulatory element Jordane Dimidschstein 135630-AAV9 pAAV-S5E2-dTom-nlsdTom AAV vector to drive...the E2 regulatory element Jordane Dimidschstein 135630-PHPeB pAAV-S5E2-dTom-nlsdTom AAV vector to drive...of the E2 regulatory element Jordane Dimidschstein 135631-AAV1 pAAV-S5E2-GFP-fGFP AAV vector to drive ...of the E2 regulatory element Jordane Dimidschstein 135631-AAV9 pAAV-S5E2-GFP-fGFP AAV vector to drive ...of the E2 regulatory element Jordane Dimidschstein 135631-PHPeB pAAV-S5E2-GFP-fGFP AAV vector to drive ...of the E2 regulatory element Jordane Dimidschstein 135632-AAV1 pAAV-S5E2-GCaMP6f AAV vector to drive the...of the E2 regulatory element Jordane Dimidschstein 135632-AAV9 pAAV-S5E2-GCaMP6f AAV vector to drive the...