...Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection You may also like... Feng...transcription factor collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors. The...the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The collection...screening transcription factors for cellular programming or cancer drug resistance. Depositor... Depositor Collections MORF...can be purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that simplifies...simplifies identification of the transcription factor isoform in pooled screens. This collection and library...

...RAR-related orphan receptor B RORC Plasmids RORC, RP11-98D18.11-001, NR1F3, RORG, RZR-GAMMA, RZRG, TOR RAR-related...peroxisome proliferator-activated receptor gamma RARA Plasmids RARA, NR1B1, RAR retinoic acid receptor, alpha...the nuclear receptor plasmid collection assembled in collaboration with the Nuclear Receptor Signaling ... Plasmid Collections Nuclear Receptor Plasmid Collection Nuclear Receptor Plasmid Collection You may also...2013. Author: Boghog2 Nuclear receptors are a class of transcription factors modulated by ligands. Two of...the receptor and results in activation or repression of target gene expression. Nuclear receptors play...androgen receptor ESR2 Plasmids ESR2, ER-BETA, ESR-BETA,ESRB, ESTRB, Erb, NR3A2 estrogen receptor 2 (ER ...

...complexes, mTORC1 and mTORC2. Each complex contains a unique subunit: raptor for mTORC1, and rictor for ...for mTORC2. Background mTORC1 & mTORC2 The better-characterized mTORC1 responds to various signals, including...Ras-related GTP binding Raptor Also known as RPTOR; Regulatory associated protein of MTOR, complex 1 Rheb Ras...plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates with ...apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine...inactivating mutations in negative regulators of mTORC1. The hyperactivation of mTORC1 in cancer promotes the conditions...kinase C Protor Also known as PRR5; proline rich 5 PTEN Phosphatase and tensin homolog Rictor RPTOR independent...

... Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors pLEG/pREG Modular Viral Vector System - Dankort... Plasmid Collections Depositor Plasmid Collections Depositor Collections ...Addgene's repository contains 157,134 plasmids, which includes plasmid collections from depositors. To help...Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection Synthetic Biology Voigt...Browse depositor collections of antibody, CRISPR, disease research, visualization, protein expression...biology, and viral vector plasmids. Plasmid...find what you're looking for, we've gathered our depositor collections on one page. We hope you find it helpful...

...Biosafety Guide Blog: Plasmids 101-Viral Vector Elements Viral Vectors 101 eBook Overview of Viral Plasmids... Lab Lentivector Toolbox Guide to Generating Recombinant Adenoviruses Eric Campeau's Viral Vector Guide...List of viral vector plasmids available at Addgene and other related resources. Special...Guide University of Pennsylvania Vector Core Roth Lab Blog, "DREADD Users Blog" For providing scientific....2010.12.009. PMID: 21167871 Wold WSM, Toth K. Adenovirus vectors for gene therapy, vaccination and cancer gene therapy...

...Structural Genomics Consortium Plasmids - Toronto Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry... from the Structural Genomics Consortium (SGC) Toronto collection Plasmid...Genomics Consortium Plasmids Browse SGC Toronto collection Structural Genomics Consortium Plasmids Structural...

...Mammalian expression vectors for TAL effector repeat arrays assembled using the REAL, REAL-Fast or FLASH...TALengineering Reagents TALEN Expression Vectors TALEN Expression Vectors for REAL, REAL-Fast and FLASH You ...This page lists plasmid vectors into which DNA fragments encoding TAL effector repeat arrays (assembled...website . All three of these methods yield TAL effector repeat arrays with identical DNA and amino acid...the final products are the same). All expression vectors listed below have the CMV promoter for mammalian...transcription of RNA. TALENs expressed from these vectors also have a FLAG epitope tag and nuclear localization...signal at their amino-terminus. The expression vectors listed below can be purchased together with the...

...Viral Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of Medicine Gene Vector Core Lentiviral...Transfer Vectors Adenoviral Vectors Kazuhiro Oka and the Baylor College of Medicine Gene Vector Core (Link...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Viral...and Adenoviral Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and ...lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this set of lentiviral...these transfer vectors and generating infectious virus. Protocol: Lentivirus Transfer Vector Production Protocol...from the PGK promoter Adenoviral Vectors The adenoviral transfer vectors contained within this set are derived...

... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors. These systems...single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs into one vector, and plasmids...expression vectors for insertion of custom gRNA target sequences. CRISPR... CRISPR Empty gRNA Vectors CRISPR Plasmids...Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...

... CRISPR CRISPR History CRISPR History and Development for Genome Engineering ...Class 1 (Multi-subunit effector complex) Class 2 (Single multi-domain effector) Type I (Cas3) Type II ...technologies that do not rely on NHEJ or HDR: Base editors : Base editors directly convert bases without creating...The first base editors converted cytidine to thymidine; newly engineering base editors convert adenosine...various CRISPR-associated (Cas) genes to not only store a record of invading phages but also to destroy ...two large classes based on the structure of the effector complex that cleaves the genomic DNA. Type II ...adenosine to guanosine. RNA editors : Cas13 fused to the ADAR2 deaminase convert adenosine to inosine on RNA...

...Viral Vector Packaging Service Penn Vector Core Transfer Viral Vector Packaging Service: Penn Vector Core...Current AAV inventory , which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics...University of Pennsylvania Vector Core (Link opens in a new window) (Penn Vector Core) and Addgene have partnered...provide high-quality AAV vectors to the academic research community. The Penn Vector Core will produce viral-based...viral-based vectors for distribution via Addgene. For a full list of Addgene's viral vectors See Addgene's...a viral vector with the Penn catalog ID: Based on low demand, production of some viral vectors have been...See the list of discontinued viral vectors . The following vectors are now available for distribution ...

...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in...transferred in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays...in-frame to these vectors. Plasmid... Plasmid Collections Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger... zebrafish) pDW1775 (for expression in plants) Vectors encoding heterodimeric FokI nuclease domains: pMLM290...

...your experiment using the lentiviral vector system. Viral vectors have been increasingly popular in fundamental...primary cells. Amongst the different vectors used, lentiviral vector constructs have proven very useful ...fluorescent sensors. Retroviral vector background Until the development of viral vectors, gene transfer into mammalian...un-transfectable cells, labs use viral vectors and particularly retroviral vectors because they target a broader...attractive choice for a gene transfer vector. Lentiviral vectors Although retroviruses are still in use...generation of lentiviral vectors consisted of three plasmids encoding 1) the lentiviral vector genome which was...lentiviral vector system offers the best safety profile in terms of RCL generation because this vector requires...

...First, some history. In 2008, the Auricchio lab decided to solve the issue of “tiny vector, big transgene... overviews of three split vector strategies plus one additional split vector strategy for increasing the...homology directed repairs (HDR). Split AAV vector approaches Split vector approaches increase the size of the...copies of both vectors. Examples of use: Halbert et al., 2002 used an overlapping vector with 440 or 1000bp...splice site donor and acceptor sequences and the homologous region of the two vectors can be from one of ... hybrid vector, as measured by β-galactosidase activity, was 81% of that from a single vector, but significantly...ideal split vector system. Overall the efficiency of transgene expression from split vectors has been inconsistent...

... the perfect voltage indicator and optogenetic actuator pair. Ideally, indicators should possess fast ...the voltage indicator, QuasAr, and actuator, CheRiff, in the bicistronic Optopatch vector, the Cohen lab... our community of depositors. With over 40,000 plasmids avaliable in the repository, we can't give all...University of Wellington, Victoria, has developed an adaptable bacterial expression vector which gives close ...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with the...

...genes, the laboratory of Jason Moffat at the University of Toronto has created the Toronto KnockOut (TKO...both laboratory and prototrophic strains. The vectors were based on the original EasyClone vectors (Jensen...coupled receptors (GPCRs) yet one third of the GPCRs in the human genome are orphan receptors meaning ...tools, compounds, and assays to monitor activation of these receptors. Bryan Roth's lab has a newly available...expression vectors with various promoters suitable for in vivo expression, and/or produce vectors to make... all TAL effector scaffolds previously constructed for the Golden Gate TALEN and TAL Effector Kit 2.0.... These vectors contain uracil-excision based (USER) sites, flanked by ADH1 and CYC terminators for easy...

...Updated mini-transposon vector for bacterial mutagenesis or gene targeting Victor de Lorenzo's lab has... existing transposon and vector systems and creating an all-synthetic vector that included only the elements...features to be monitored to study mitochondrial dysfunction. Easy-to-use tools to monitor these features... into a destination vector in a predefined order. Finally, the destination vectors are used with Multisite... eight entry vectors can be used for each Multisite Gateway compatible destination vector for a maximum...optogenetics tools – light-activated receptor tyrosine kinases Receptor tyrosine kinases (RTKs) are a large...step, compatible Level 0 vectors are directionally assembled into a Level 1 vector creating a single transcriptional...

...polycistronic vector or multiple single-gene vectors can be used to package reprogramming factors. An important... OSKM reprogramming factors: Mouse Expression Vectors Human Expression Vectors Carey et al Hockemeyer...reprogramming vectors available from Addgene. Another drawback is that lentiviral vectors insert in different...Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...Non-integrating iPSC delivery methods Viral Vectors Adenovirus: Adenoviral vectors infect dividing and nondividing...different adenoviruses, each expressing one factor. These vectors don’t integrate into the genome and are ...mutagenesis, adenoviral vectors are considered a safer way to express reprogramming factors for therapeutic applications...

...building successful mentor relationships, asking for mentor support, and choosing your mentor. What isn’t discussed...receive technical mentorship from a ‘bench mentor’. In some instances, the bench mentor is also the scientific...Scientist Edition Mentoring for Scientists: I Have a Mentor, What Now? Will You Be My Mentor? Finding and ...often enough is the hands on mentoring you receive after you’ve found your mentor, though. Typically, you ...scientific mentor…but usually the bench mentor is a staff scientist, postdoc, lab manager, technician, or...more, depending on the mentoring style within the research group). The bench mentor-mentee relationship ...lot more face time with your bench mentor than your scientific mentor. Thus, this relationship will probably...

... A cis-splicing vector served as positive control (cis-ctrl). v1, vector 1; v2, vector 2; BD, binding ...Improved prime editor PE7 Antibodies to facilitate SARS-CoV-2 research New viral vector preps of Voltron2...Voltron2 voltage sensors AAV vectors for targeting microglia The REVeRT Dual AAV Vector System Neurodegeneration...protection factor) promotes editing (Yan, J et al., 2024). They developed a new prime editor that incorporates...Brian O’Neill New viral vector preps expressing the Voltron2 voltage indicator, created by the Schreiter...voltage), the investigators found that administering these AAVs with a dilute Cre-expressing vector resulted...incorporated in an AAV vector (Figure 5). Efficiency and specificity of these AAV vectors for microglia was...