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We narrowed to 770 results for: VARS

Showing: 121 - 140 of 770 results

  1. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...Campbell’s lab added two new mCherry variants to the repository. These variants can be used as tools to learn... Podcast Segment The impact of KLF4 N-terminal variants on iPSC generation Deriving iPSCs (induced pluripotent...polycistronic cassette. However, it was unknown whether variations in gene expression from these polycistronic cassettes...transposons, the Woltjen Lab compared different variants of the polycistronic cassettes and discovered ...which they traced to a 9 amino acid N-terminal variation in the Klf4 isoform (KLF4s or KLF4L). Polycistronic...discovered that monocistronic expression of either variation of Klf4 did not lead to the observed reprogramming...Dlx5/6) enhancer elements and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e. DREADDs...

  2. Components of CRISPR/Cas9

    Type
    Blog Post
    ...' TTTV AsCpf1 RR variant 5' TYCV LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter... of these different systems can vary (for example, xCas9 is a variant with increased PAM flexibiliy and... PAM sequence. Cas9 Species/Variants and PAM Sequences Species/Variant of Cas9 PAM Sequence ...NGG SpCas9 D1135E variant 3' NGG (reduced NAG binding) SpCas9 VRER variant 3' NGCG SpCas9...SpCas9 EQR variant 3' NGAG SpCas9 VQR variant  3' NGAN or NGNG  xCas9 3' NG, GAA, or GAT SpCas9... will be able to describe the functions of the various CRISPR/Cas9 components. Please note that while ...general overview of CRISPR components, new Cas9 variants are being discovered all the time and the requirements...

  3. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...controls are invariably inconclusive and unreliable. Proper controls provide the constant variables that enable...independent variable. Referring back to the experiment associated with Figure 1, the independent variable is the...every day. Although the designs and outcomes may vary, one thing should be present in every experiment-based...interpretation of the effect of the independent variable you are testing. Importantly, they demonstrate...your experiment. Read on to learn more about the various controls that can be used for plasmid-based experiments...is specifically associated with the independent variable you are testing and not some other unintended ...to minimize any effects of the non-independent variables.  To illustrate the importance of control plasmids...

  4. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...green (v857) variants differing in kinetics, affinity, and ∆F/F0. Introduce the two variants in three different...and other brain cells in vivo. Glutamate plays a variety of roles in synaptic communication and can trigger...for sfGFP to create SF-iGluSnFR. This improved variant has higher expression levels in bacteria and produces...models. Furthermore, they created three affinity variants – A184S (increased affinity for glutamate), S72A...Kaspar Podgorski’s lab have recently made iGluSnFR3 variants that are more sensitive and better localized for...predecessors. The detailed characterization of the new variants is available here. Upgrades to iGluSnFR3 To improve...Improved photophysical properties The improved variants of iGluSnFR3 have higher expression levels and...

  5. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...protocol may vary depending on your specific experiment, but a broad overview of the various ways in which...your experimental target, SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check...our SpCas9 containing plasmids, optimized for a variety of species.   Specificity concerns? If off-target...regions of the genome and consider using a Cas9 variant with higher specificity (eSpCas9 and HypaCas9) ...added bonus of low off-target effects. The SpG variant, discovered by the Kleinstiver group has a PAM ...Further, this group also characterized a second Cas9 variant, SpRY, which cuts at NRN PAMs with high efficiency...a small window, this system can be used with a variety of impaired Cas9 proteins (such as SpRY) with altered...

  6. Pipetting Protocol

    Type
    Protocol
    ... stop flowing into the pipette tip. Liquids of various viscosity have different flow rates. The more viscous...

  7. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...of interest in a variety of model systems, thereby opening your research to a variety of experimental ...sequencing (pInitial) and protein production (a variety of pExpression vectors) plasmids. The type IIS .... Expression vectors within this kit contain a variety of promoters to facilitate gene expression in bacteria...oocytes. The expression vectors additionally contain various combinations of protein tags (His, Avi, and GFP...plasmids in this kit have been used to purify a variety of peptides including antibiotic efflux pumps and...created this kit. The plasmids within it contain a variety of different promoters followed by leader sequences... at the 3’ end of the leader sequence. Using a variety of GFP and RFP fusions as their test genes, the...

  8. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...’s lab in Novartis Oncology.  Bhang did her PhD work at Johns Hopkins and came to Novartis for her post-doc...research. When she came to Novartis, she was happily surprised that “Novartis was actually really research...This article is based on an interview with Novartis researcher, Carrie Bhang. The ClonTracer Library,...investigator in the In Vivo Pharmacology group at Novartis Oncology, is an exciting new tool that allows ... and says that she decided to do a post-doc at Novartis because she wanted first-hand experience in industry...co-workers started using the tool to look at a variety of questions surrounding cancer development, metastasis...investigator in the In Vivo Pharmacology group at Novartis Oncology, Bhang has been working on using the ...

  9. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Kim et al., 2017 SaBE3, BE3 PAM variants, BE3 editing window variants   Greatly expands the number of ...increased editing frequency to above 30% for a variety of targets in human cell lines, with an average...upstream of the PAM.  David Liu’s lab generated BE3 variants with other deaminases: AID, CDA1, and APOBEC3G... Liu lab also used natural and engineered Cas9 variants to develop five new base editors with distinct...HF-BE3, a base editor containing high fidelity Cas9 variant HF-Cas9 (Rees et al., 2017). HF-BE3 showed 37-fold...Gaudelli evolved the base editor into 40 new ABE8 variants (Gaudelli et al., 2020). Compared to ABE7.10, ...-fold higher editing efficiency at non-NGG PAM variants compared to ABE7.10. ABE8s have an improved base...

  10. Hot Plasmids February 2024

    Type
    Blog Post
    ...library of AAV9 variants (Chuapoco et al., 2023). They found the best-performing variant, CAP-Mac, was ...genome editing for primary cells Monomeric StayGold variants AAV.CAP-Mac for systemic gene transfer in primate... Breaking up is hard to do: Monomeric StayGold variants By Mike Lacy In 2022, Atsushi Miyawaki and colleagues...stability. Here’s a quick overview of these new variants:   Figure 2: Inner mitochondrial membranes...QC2-6 FIQ or mStayGold (Ando et al., 2023). This variant has similar photostability and brightness compared...Ivorra-Molla et al., 2023). They also named their variant mStayGold. It is worth mentioning that Kiryl Piatkevich...colleagues generated yet another monomeric StayGold variant (Piatkevich et al., 2023). Check out their preprint...

  13. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...immune system and a tool that can be used for a variety of research applications. Within the immune system...regions are completed with the Ig light chains. The variable region of the heavy and light chain determine ...Fab, heavy chain, light chain, constant region, variable region   The constant region of the heavy chain...antibody depends partially on its isotype. The variable region of an antibody does not recognize the entirety...antibodies are extracted. These antibodies recognize a variety of epitopes on the target protein, so they are ...antibodies against different protein epitopes and so variability between lots is a big concern. You can find more...clonal population of cells that produce a single variant of antibody. Monoclonal antibodies are less likely...

  14. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...naturally-occurring variations in the TALE repeat, referred to as “non-RVD variations”, were found to improve...successfully create GFP fusion proteins with a variety of genes across the genome. Although this tool ...characterization, has enabled scientists to engineer a wide variety of GFPs with diversity in fluorescence brightness... engineering, Ed Boyden’s lab has identified a variety of new channelrhodopsins for use as optogenetics...which is 45 nm more red-shifted compared to other variants. The group further optimized Chrimson through ...off-kinetics (15.8 ± 0.4ms from 21.4 ± 1.1 ms); this new variant was named ChrimsonR. Plasmids containing Chronos...lentiviral and AAV expression vectors for certain variants. Klapoetke et al., Nat Methods 2014 Mar 11;(...

  15. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ... and mouse model systems, testing base editing variants, and assessing the safety of their intervention...activity, which can cause off-target effects in a variety of RNA transcripts independent of Cas9 targeting...RNA-editing activity and developed a deaminase variant with a V106W mutation, which reduced RNA editing...in human cells on average, but its effectiveness varied depending on the chromosomal location, sequence... al., 2020).  Five years later, three of these variants (ABE8.8, ABE8.20, and ABE8e) would be tested for...problem, Benjamin Kleinstiver’s lab engineered new variants of S. pyogenes Cas9. While the wild-type enzyme...where N represents any base), their engineered variants are more permissive: SpG can target NGN PAMs, ...

  16. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...observations of oxytocin dynamics in live mice under a variety of behaviors, opening up new research directions...other MTRIA sensors based on GPCRs recognizing various neurotransmitters, neuropeptides and other ligands...circularly-permuted GFP into an intracellular loop of various G-protein-coupled receptors. Image adapted from...for synaptic glutamate response. They generated variants optimized for different applications (v82 has ...faster kinetics but lower affinity) and with a variety of tags for localization (PDGFR, GPI, and SGZ).... and powerful high-throughput screen to select variants based on performance metrics specifically for ...using potassium-binding protein homologues from various bacteria led to sensors of intermediate affinities...

  17. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    ...Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland.... Crystal now works on protein therapeutics at Novartis - listen to learn more about Crystal’s exciting...Crystal's educational background and position at Novartis. Chapter 2: What's a lab group in industry? The...empower people Chapter 3: How Crystal advanced at Novartis, and how to prepare yourself for success by having... The types of science that Crystal works on at Novartis, how she's learned to prioritize projects, and...listings. Mobility between different positions at Novartis. Chapter 7: How Crystal developed her science ...communication skills and how they've helped her at Novartis. The importance of mentorship in developing career...

  18. Technical Design of a Western Blot

    Type
    Blog Post
    ...signal. However, they have high cross-reactivity, vary significantly from lot-to-lot, and have to be re-validated... plasmids and have very little batch-to-batch variation. Confirmation of sequence can be done from relatively...sizes.  Table 2: Recommended gel percentages for various protein sizes Protein Size Target Proteins...capacity and high protein retention. It comes in a variety of pore sizes and protein capacity, allowing you...lot of work around a flame. Membranes come in a variety of pore sizes. If your protein of interest is small...dry/electroblot transfer systems available from various manufacturers. These proprietary systems are fast...are worth considering if your proteins of interest vary wildly in size. However, these systems are expensive...

  19. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...to try developing a variant with an enhanced response. Evolution of a new variant Maria and the team set... clear that this was even possible. There is a variety of research on whether and how organisms respond...mutagenesis and screening, the EGFP and mScarlet variants were showing no obvious signs of improved response...deprioritize the project, Maria’s screens of AsLOV2 variants showed some significant gains! After a few more...the magnetic field (Figure 2); they named this variant MagLOV. Andrew was thrilled to see that MagLOV’...: Fluorescence intensity and ΔF/F of MagLOV, a variant of AsLOV2 with mutations C450P, L496V, Q513K, G528K... Andrew and Maria expect that further-improved variants of MagLOV will be possible with other engineering...

  20. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...levels.   Overview of Variations Between Brainbow Systems   Brainbow variants and applications beyond... enables Brainbow to be used in a variety of species. More variants have been created by other labs, ...2007, Joshua Sanes and Jeff Lichtman, both of Harvard’s Department of Molecular and Cellular Biology, ...expression; these deletions are defined using 2 loxP variants (only identical loxP sites can mediate recombination...either deletion removes one of the other loxP site variants, preventing further recombination. Brainbow-1.1...with recombination events specified by three loxP variants, but the principles remain the same. The first...expect ten colors (see table to the right); in various reports, Sanes and Lichtman have observed from ...
Showing: 121 - 140 of 770 results