Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 397 results for: abo.4

Showing: 121 - 140 of 397 results

  1. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...optogenetic implants have been reported by several groups [4, 5, 6]. These have the obvious advantage of not requiring...PMID: 23042500; PubMed Central PMCID: PMC3466483. 4. Kim TI, et al. Injectable, cellular-scale optoelectronics...distance so ideal implant placement should be about 0.4-0.5mm above the site of your stimulation. The scatter...scatters a great deal once it enters tissue, placement above the site of stimulation guarantees that the majority...instead). Laser stimulation         In the video above, Channelrhodopsin 2 (Chr2) has been expressed in...These lasers are relatively cheap and will run you about $2-3,000/laser. However, an important caveat is ...output of the laser must be controlled. As discussed above, each opsin variant has slightly different activation...

  3. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...to control the event is what is important. Choose 4 panelists (maybe 5) and no more. Too many panelists...moderator and organizers should develop a list of 4-6 pre-event interview questions. The moderator (possibly... are nice if you can afford them). I am talking about making sure the audience is interested, educated...of the stories or topics you know they can speak about. There should be some spontaneity, but having a ...location and parking information and remind them about the event as it approaches. Room set-up and logistics... make or break the discussion section. And what about those audience members that want to take over? It...

  4. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...sites interspaced with random sequences.   Strategy #4 (ProD): Create promoters with the help of epigenetic...different promoter designs, promoters from strategy #4 had the highest rate of targeted expression. Surprisingly...Addgene blog Read more about using AAV for retinal gene therapy Find more about precise neuronal targeting...injected into macaques and postmortem human retinas. About 25% of these AAV synthetic promoters successfully...

  5. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...process since May 2020. We find this process takes 4-6 weeks on average. Therefore, we recommend placing...expedite the application process.  More information about export control licenses is available through the...Industry and Security. If you have any questions about this or other Addgene shipping and ordering processes...customer support team at [email protected].  Curious about how rabies plasmids are used in the lab? Check out...

  7. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...biological history. 4. Please Don't Kill My Hive Beautifully filmed video about colony collapse disorder...Science with Tom. In this podcast, we learn more about Tom and pick his brain for advice on how to dive... Rap of Henrietta Lacks Students in Oakland rap about Henrietta Lacks - the African American woman whose...science standards and communicate science. Learn more about Tom on his youtube channel and at sciencewithtom.com...

  8. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...microenvironment produce CTL associated protein 4 (CTLA-4), a receptor that turns off T cell function upon...immunology 3.11 (2002): 991-998. PubMed PMID: 12407406. 4. Weiner, George J. "Building better monoclonal antibody-based...potential beneficiaries. Collaborative therapy For the most efficient outcomes, collaborative therapies must be... of the tolerance-producing antigens enunciated above. Finally, signals released from the microenvironment...are in their clinical trial phase. In all of the above therapeutics, techniques like genome engineering...are beginning to see the fruits of many years of labor with up and coming immunotherapy treatments. As ...clinic, physicians and researchers are learning much about how treatment regimens can be improved and how the...

  9. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...see whether and to what extent they overlap. Figure 4 compares the spectra of enhanced GFP (eGFP) and FITC...be used within the same panel.     Figure 4: Excitation and emission spectra for eGFP and FITC...channel to use to detect eGFP. As you saw in Figure 4, eGFP strongly overlaps with FITC, hence reading out...spectrum is located in a range of wavelengths of about 380–700 nm. Photons of lower wavelengths are higher... 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If we were to use a laser that excites at...optimal excitation happens at higher wavelengths of about 750 nm. Source: https://www.bdbiosciences.com/en-us... we start building your first panel, we'll talk about one last principle of color emission. As you can...

  10. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...analysis example is given in Figure 2 (adapted from 4) - Dunn et al. 2011 provides an in-depth review of...CureFFI.org. Cell Biology 04: The Secretory Pathway. 4. Dunn, Kenneth W., Malgorzata M. Kamocka, and John...the context of the cell. However, much knowledge about functionality can be gained by understanding the...subcellular marker proteins, it’s possible to get an idea about the subcellular structures your protein resides ...with. These “colocalization” studies give insights about the proximity of two proteins within the same subcellular...Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous Gene Tagging in Human Cells...

  11. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    ... conversation with your manager early on. Chapter 4: Crystal's approach to management and the importance...therapeutics at Novartis - listen to learn more about Crystal’s exciting career path, to gain insight ...Chapter 2: What's a lab group in industry? The collaborative nature of working in industry and how delegating...metrics for success. The importance of thinking about the big picture and not just the next experiment...resources in our Mentoring for Scientists Series Learn about Turning Your PhD into a Career with Joanne Kamens...

  12. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the 3’ end of the target sequence... Find plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah...a cell culture model. What we still don't know about Cpf1 In their original paper characterizing Cpf1...Addgene Blog Catch Up On Your Cpf1 Background Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac...

  13. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...capable of recruiting additional effector domains (4). Taken together, these minor gRNA alterations can...PMID: 26344044. PubMed Central PMCID: PMC4666719. 4. Mali, Prashant, et al. "RNA-guided human genome engineering...of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing scientific... Listen to Our Interview with Alex Chavez Learn about Sequencing Options for CRISPR Genotyping Cas9 Activators...

  14. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...top ten countries for each group (Figure 4).    Figure 4: Addgene deposits and distribution by country...with Addgene by year. (C) Cumulative number of laboratories that have deposited CRISPR plasmids by year....

  15. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...phosphorylated DNA termini to 3 ́-OH DNA termini (1-4). In vitro, ligases (notably T4 DNA ligase) are critical...PMID: 18518823. PubMed Central PMCID: PMC2933818. 4. Shuman, Stewart. "DNA ligases: progress and prospects... And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes that seal breaks in ...to accelerate characterization of nucleic acid metabolic enzymes." Nucleic acids research 44.2 (2015): ...

  16. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...Cardiovascular Research, 1(4), Article 4. https://doi.org/10.1038/s44161-022-00046-4 Kumar, S. R., Miles, T... fold change in RNA and DNA levels (~25-fold and ~4-fold respectively) relative to AAV9 in the pigtail...nervous system. Current Research in Neurobiology, 4, 100086. https://doi.org/10.1016/j.crneur.2023.100086...106–115. https://doi.org/10.1038/s41593-021-00969-4 Goertsen, D., Goeden, N., Flytzanis, N. C., & Gradinaru...enhanced transgene expression in the macaque CNS. Med, 4(1), 31-50.e8. https://doi.org/10.1016/j.medj.2022.11.002... for their use. Then, we’ll provide information about commonly used and emerging capsids to help you decide...our Caltech Systemic Capsids page to learn more about that collection. Table 1: Systemic capsid plasmids...

  17. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...spread (metastasize) and turned into Stage 3 or Stage 4 cancer. These later stages of a cancer diagnosis indicate... is large in size and may have spread while Stage 4 indicates that the cancer has spread from where it...common cancers like lung (about 224,000 diagnosed this year) or breast cancer (about 246,000 diagnosed this...Mesothelioma Cancer Alliance. We have learned much about the causes of cancer and the different avenues that... asbestos exposure to the time of diagnosis) is about 35 years, but it usually ranges from 20-50 years... an orphan cancer; it is rare disease with only about 3,000 new mesothelioma patients diagnosed every ...from mesothelioma several years ago – recently collaborated with Senator Barbara Boxer to introduce the ...

  18. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...38 kb Cells HEK-293T Sf9 Time to collection 2–4 days 5–10 days Purification Ultracentrifugation ...frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077.... widely available, so you’ll only have to worry about cloning your transfer plasmid. The transfer plasmids...typical insect cell preparations, which we talk about below. Processing The viral supernatant is typically...available, so the amount of cloning required is about the same between insect and mammalian systems. Baculovirus...dead cells and cellular debris. While adding time (about three to four days), this second round provides ... can skip the first round of production, saving about a week of work. An important note is that this is...

  19. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ... ultimately getting interpretable results: Figure 4: Expression Level of Gene X   The design and selection...it significant? Vaux DL, et al. EMBO Rep. Apr; 13(4): 291–296. (2012). PubMed Central PMCID: PMC3321166...optimization within your experiment. Read on to learn more about the various controls that can be used for plasmid-based...: Expression Level of Gene X The results shown above are from a single experiment wherein Plasmid A (...be compared. The Empty Vector Control (mentioned above) could also serve as an important negative control...experimental cells. For example, in the experiment above, Plasmid A contains an shRNA that targets human ... times and on different days. Using our example above, we would test different preparations of Plasmid...

  20. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...library consists of 77,441 sgRNAs with an average of 4 sgRNAs per gene and 1,000 non-coding control sgRNAs...PMID: 25307932. PubMed Central PMCID: MC4253859.  4. Konermann, Silvana, et al. "Genome-scale transcriptional...Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the advantages of pooled...large-scale functional screens Read this blog post about genome-wide screening using CRISPR Resources on...libraries for the mouse genome! Other Resources Read about CRISPRi and CRISPRa from the NIH E-newsletter ...
Showing: 121 - 140 of 397 results