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We narrowed to 231 results for: antibody

Showing: 121 - 140 of 231 results
  1. Inntags: Innovative Protein Epitope Tagging

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    Blog Post
    ...expression level without the need for a custom antibody. There is one major caveat - a tag may interfere...affinity for other proteins, and accessibility to an antibody. From their initial pool of candidates, they selected...
  2. Antibodies 101: Flow Compensation

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    Blog Post
    ...your samples with multiple antibodies, the fluorescent probes on the antibodies may have similar emission...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Conventional vs. Spectral...this handy emission spectra chart to find your antibody's spectra). Your cytometer will therefore record...BioLegend’s Fluorophore Guide Chart.  Pro tip! Some antibodies go by different names depending on your flow ...
  3. Great Results Start with Great Standard Curves

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    Blog Post
    ...Addgene's Antibody Guide Addgene's Ready-to-Use Antibody collection Addgene's Recombinant Antibody Plasmids...below 0.95! More resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and When to Use Them...
  4. Plasmids 101: Biotinylation

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    Blog Post
    ... and antibody labelling. In these experiments, biotin is added to a primary or secondary antibody that...that targets your protein of interest or primary antibody respectively. Avidin bound to a reporter is then... on this complex can bind to your biotinylated antibody. This creates a large meshwork of biotin-avidin...
  5. Addgene at SfN23!

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    Blog Post
    ...Addgene's booth? Well, first, Addgenies! We'll have an antibody specialist, Amrita, and a viral vector specialist...tons of information about Addgene's ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus...
  6. Brain Initiative Collection

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    Collection
    ...recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use recombinant antibodies made from plasmids...partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity Recommended Applications...monoclonal antibodies validated for neuroscience research. The plasmids encoding these antibodies are available...Initiative collection of plasmids, viral preps, and antibodies here. Collections...community. Browse plasmids , viral preps , and antibodies created with support from the BRAIN Initiative...plasmids in our collection! These monoclonal antibodies are produced in-house and undergo application-specific...
  7. Getting to Know Expansion Microscopy

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    Blog Post
    ...on the Addgene blog Antibodies 101: Introduction to Immunofluorescence  Antibodies 101: Multiplex Immunofluorescence...ExM protocols are compatible with conventional antibodies, chemical dyes, and genetically encoded fluorescent...microscopy using fluorescent Fab fragment secondary antibodies. Methodsx 9:101796. https://doi.org/10.1016/j.mex...labeled using standard fluorescent proteins and antibodies. Nat Biotechnol 34:987–992. https://doi.org/10.1038...Immunofluorescence Antibodies 101: Affinity Reagents ...
  8. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post
    ...transcription factor IRF-1. Immunoprecipitation, using an antibody targeting the 3xFLAG tag, allowed these researchers...well as affinity purification with an anti-Cas9 antibody. These different tag systems and affinity purification...similar affinity purification with an anti-Cas9 antibody, the Li lab found a microRNA (miR483) that binds...
  9. Plasmid Collections

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    Collection
    ...γ-Retrovirus Adenovirus All Viral Plasmids Curated Topics Antibody Plasmid Collection Bacterial Expression Systems...
  10. Antibodies 101: Reading a Flow Plot

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    Blog Post
    ...Plasmid-based Recombinant Monoclonal Antibodies Introduction to Antibodies  ... is a cell, but it could be debris or unbound antibodies.) Each event read by the machine is plotted as...using a panel of markers, which is comprised of antibodies conjugated to fluorescent markers and/or cellular...  In the third plot, anti-CD14 and anti-HLA-DR antibodies conjugated to fluorescent markers are used to... three different populations using conjugated antibodies against CD11c and CD123. You can see that mDCs...
  11. 2022 and 2023: Reflections and Looking Forward

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    Blog Post
    ...ready-to-use recombinant antibodies collection, currently composed of over 100 antibodies. Everything in this...which includes both neuroscience and general-use antibodies, is available in both trial- and full-size, with...
  12. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post
    ...going to be looking for recombinant monoclonal antibody plasmids from the James Trimmer Lab published ...distributing 94 plasmids expressing validated antibodies for neuroscience research (see the NeuroMab Collection...specific plasmid from the NeuroMab collection for an antibody against PARIS, a zinc finger protein involved ...
  13. Zebrafish Plasmid Collection

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    Collection
    ...for Zebrafish Receptors and Zebrafish Monoclonal Antibody plasmids - Gavin Wright Lab. Collections of plasmids...that encode zebrafish receptors or monoclonal antibodies to zebrafish cell surface receptors and secreted...
  14. Hot Plasmids: Summer 2025

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    Blog Post
    ...months we highlight some of the new plasmids, antibodies, viral preps, and more in the repository through...ATLAS (anterograde transsynaptic label based on antibody-like sensors) changes that. Developed by the Arnold... Arnold Lab, ATLAS is based on an antibody-like protein AMPA.FingR (AF) that binds to AMPA receptor subunit...
  15. SARS-CoV-2 Pseudotyped Virus

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    Collection
    ...Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response Against Pseudoviruses ...
Showing: 121 - 140 of 231 results