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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 315 results for: bon;

Showing: 121 - 140 of 315 results

  1. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...six consecutive His residues (or more). Histidine bonds with immobilized metal ions (Nickel, Cobalt, Copper...

  5. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...digested vector backbone. This is accomplished by covalently connecting the sugar backbone of the two DNA..., the insert DNA is physically attached to the backbone and the complete plasmid can be transformed into..., it is important to treat the digested vector backbone with a phosphatase before performing the ligation...

  13. Plasmid Cloning by PCR

    Type
    Blog Post
    ...MCS)), but do not cut elsewhere on the plasmid Bonus: It is helpful to choose restriction enzymes that...

  14. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...S. pyogenes de Bono sgRNA with rpr-1 promoter 48961 Worm EcoRI none S. pyogenes de Bono pMB60 47941 Worm... cut S. pyogenes Puro Zhang sgRNA(MS2) cloning backbone 61424 Mammalian BbsI none S. pyogenes Zhang pSpCas9n...none S. pyogenes Puro Zhang lenti sgRNA(MS2)_zeo backbone 61427 Mammalian/Lentiviral BsmBI none S. pyogenes...BsmBI none S. pyogenes Joung AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR 68347 Mammalian... cut S. pyogenes mCherry Kuhn AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR 60229 Mammalian/AAV SapI... SapI none S. pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR 60231 Mammalian...AAV SapI none S. pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 60226 Mammalian...
Showing: 121 - 140 of 315 results