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We narrowed to 166 results for: control GFP

Showing: 121 - 140 of 166 results

  1. CRISPR References and Information

    Type
    Collection
    ...vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (...packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification...packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Pooled libraries are also available for human...Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles...

  2. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...contrast, the widely used green fluorescent protein (GFP) gives a bright green fluorescence when exposed to...genome, it is not expressed as it is under the control of a rhamnose-inducible promoter. The resolution...eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple...

  3. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...destination vector contains GFP, enabling you to select cells with high GFP expression. These cells have...cannot be released, adding temporal and/or spatial control to the system. pSQT1313 from the Joung lab allows...

  4. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...apart. While the classic Green Fluorescent Protein (GFP) and other fluorescent proteins revolutionized live-cell...Proteins involved in endoplasmic reticulum quality control navigate a large and interconnected system of dynamic...the sensitivity of HiBiT with Addgene's quality control, support, and distribution capabilities. Give HiBiT...

  5. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...and labeled with primary antibody (0.01 µg/mL Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA...channelrhodopsin-2 or fluorescent reporter dTomato under the control of one of seven enhancers specific for different...expression of dTomato reporter (magenta) under the control of the BiPVe3 enhancer and parvalbumin (PV) immunostaining...Addgene cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene...excited to share our very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed right here... packaging and strong expression after inserting EGFP and provide step-by-step protocols for viral generation...Figure 2). Looking for more? Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set...

  6. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...modifications in the same strain. MazF expression is under control of the AOX1 promoter whose expression is induced...used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed mutations,...

  7. The Challenges of Cell Culture

    Type
    Blog Post
    ... laboratories to carry our independent quality control for a range of testing, from animal doping to forensics...Mammalian Cells Learn about the Dangers of Using GFP for Protein Localization Resources on Addgene.org...

  9. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-...world and corrective approaches and appropriate controls are required to make this method useful for dynamic...

  10. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...overcome by using a Tet-inducible virus to allow for controlled expression of reprogramming factors. Check out...the presence of transgenes is still an important control. Additionally, Sendai virus doesn’t rely on promoters...that multiple transfection makes it difficult to control the dose of plasmid the cells receive over the ...cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution...Ohtaka, and Mahito Nakanishi. "Novel Strategy to Control Transgene Expression Mediated by a Sendai Virus-Based...

  12. Antibody Plasmid Collection

    Type
    Collection
    ...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...find antibody plasmids for: Common antigens such as GFP or mCherry Monoclonals, Nanobodies, Sybodies, or ...peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies and their...

  13. Recombinases AAV Preps

    Type
    Collection
    .../Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP 9, rg* James M. Wilson 105558...site-specific recombinases, which can be used to control gene expression. See our Cre-lox Plasmids page ...9, rh10 James M. Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 James M. Wilson 55632...tdTomato 9 William Pu 203842 pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE EF1a EGFP 8 Ivo Spiegel 105550 pAAV.GFAP.Cre.WPRE.hGH...Syn EBFP 5 Hongkui Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP 1, 2, 5, 8, 9, rg*, PHPeB James...

  14. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...experiment, consider co-transfecting with GFP. This allows you to sort for GFP-positive cells and to enrich for...Engineering Using CRISPR/Cas Systems has expanded out of control. To further consolidate these FAQs, Le Cong worked...

  16. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...aptamers able to bind to a small molecule derivative of GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone...metabolites, leading to conformational changes that control the expression of coding RNA. Spinach-based riboswitches...

  17. Genetic Code Expansion

    Type
    Collection
    ...Always express first with a control reporter gene: GFP for E. coli or mCherry-GFP for mammalian cells. You...aminoacylate the orthogonal tRNA. Therefore many controls must be used to make sure that these conditions...Mammalian TAG Peter Schultz 50831 pAcBac2.tR4-OMeYRS/GFP* tyrosyl-tRNA synthetase E. coli various unnatural...or DiZHSeC Mammalian TAG P. Chen 92047 pCOTS-pyl-GFP(35TAG) PylRS M. mazei Cyanobacterial TAG Lital Alfonta...analogs Mammalian TAG Huiwang Ai 160041 pRaGE Pyl TAG GFP Y35TAG PylRS M. mazei Bacterial TAG Lital Alfonta... Nanxi Wang 240124 pIDTsmart-MbPylRS-4xPyOtR-ITR-GFP PylRS M. barkeri Azidolysine (AzK) Mammalian, AAV...C321.Ub-UAG-sfGFP all TAG sites changes to UAG, RF1 function removed, with Ubiquitin-UAG-sfGFP reporter ...

  18. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...26973 AAVrg pAAV-hSyn-hChR2(H134R)-EYFP   Control AAV Control AAV allow researchers to not only interpret...inventory. Our new AAVs include: Optogenetics AAV Control AAV Biosensor AAV And more! Optogenetics Optogenetics...more efficient tools for light mediated neuronal control and we are expanding our viral service to make ...own experiments. To this end, we offer specific control AAV in smaller aliquots of 20 ul for serotype testing... service has now expanded to offer many of our control plasmids in new serotypes. See our Serotype Testing...response to action potentials, is now offered under control of the CAG promoter for ubiquitous expression. ... 44362  AAV1 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Controls Plasmid Serotype Name 50459 AAV1 pAAV-hSyn-DIO-mCherry...

  19. New Viral Vectors - March 2024

    Type
    Blog Post
    ...pAAV-hDlx-Flex-dTomato-Fishell_7 AAV2 Controls Fishell New serotype pAAV-mDlx-NLS-mRuby2  AAV PHP.eB Controls Gradinaru New ...pAAV-CaMKIIa-mCherry  AAV PHP.eB Controls Deisseroth New serotype pAAV-Ef1a-mCherry  AAV PHP.eB Controls Deisseroth New... pENN.AAV.EF1a.eGFP.WPRE.rBG AAV PHP.eB Controls Wilson New serotype pAAV-CaMKIIa-EGFP AAV PHP.eB Controls...Deisseroth New viral vector pAAV.CMV.PI.EGFP.WPRE.bGH  AAV PHP.eB Controls Wilson New viral vector pENN.AAV.EF1a.eGFP.WPRE.rBG...

  20. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... of a magnetoresponsive fluorescent protein. Controlling an optogenetic or fluorescent tool with magnets... 2016), and some previous attempts at magnetic control of biological systems (Wheeler et al., 2016; Duret...T., York, A. G., Ingaramo, M. (2024). Magnetic control of the brightness of fluorescent proteins. Zenodo...Güler, A. D. (2016). Genetically targeted magnetic control of the nervous system. Nature Neuroscience, 19(...protein they tested — E. coli expressing good ol’ EGFP — showed a slight but detectable change in fluorescence...better, multiple other labs have reproduced their EGFP findings since the initial report in 2023.)   ...   Figure 1: Fluorescence intensity of EGFP-FlavinTag in E. coli changes in response to a ~25 mT...
Showing: 121 - 140 of 166 results