We narrowed to 660 results for: epo

...readers may locate data supporting the research reported in PLOS publications. The policy was not an overnight...is calculated and placed in the legend. Widely reported difficulties in reproducing experimental data ...datasets are too large to transfer to a journal repository, they are likely too large to be retrieved by...Scientific Data, which aims to act as a peer-reviewed repository for “scientifically valuable” datasets. It likely...likely won’t be long before other data repositories emerge that allow efficient searching and extraction...

...only 46% could be reproduced. In a recent Nature report from researchers at Amgen, it was stated that only...through all saved data and automated writing of reports. To be able to understand the situation in depth...keep paper records as well as digital, or for reporting on a project – printing your data out can be required... you might need share data with them including reports and protocols. To facilitate this, some ELNs allow...Software for Biology Resources on the Addgene Blog Deposit your plasmids with Addgene and make your work more...

...Russell and Hirata from the University of Washington reported a surprising finding - when delivered via AAV ...take up ~6.5 kB if delivered in a single AAV. They report roughly 40% repair efficiency in the K562 leukemia...develop AAVs for your own research and encourage to deposit any new AAV tools you create. Many thanks to our...AAV vector cures haemophilia B mice." Scientific Reports 7.1 (2017): 4159. PubMed PMID: 28646206. PubMed... gene cassettes using AAV donor vectors." Cell reports 20.3 (2017): 750-756. PubMed PMID: 28723575. PubMed...

...Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope ...pHCMV-RabiesG Contains Rabies virus G protein; see depositing paper for more information Miguel Sena-Esteves...pcDNA-SADB19L Contains rabies viral RNA polymerase; see depositing paper for more information Edward Callaway ...tools for AAV tracing, made from rabies plasmids deposited by Ian Wickersham’s lab....

... When we started in 2004, we were the plasmid repository. In 2016, we added viral vectors, and in 2022...ready-to-use antibodies. Now, we think of ourselves as a repository for DNA-based materials, including our popular... kits and libraries. This DNA-based materials repository contains 139,868 reagents from 5,821 labs in ... system for scientists using materials in our repository, from our customer and science support to our...

... targeting astroglia. It has additionally been reported to have tropism for liver, vascular endothelial... muscle inflammation and degeneration has been reported (Muraine et al., 2020). AAV8 AAV8 has high transduction... the CNS (Han et al., 2023), and has also been reported to have tropism for liver and kidney cells (Issa...Use Addgene’s AAV Data Hub to find experimental reports on commonly used vector cargo designs and administration...cells utilizing various AAV serotypes. Scientific Reports, 15(1), 20927. https://doi.org/10.1038/s41598-025...

...at specified loci. Charles Gersbach’s lab has deposited mammalian expression constructs including pcDNA-dCas9...Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for...Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9...Demethylase 1 (LSD1) Tatjana Sauka-Spengler's lab has deposited pX330a dCas9-LSD1 for targeted removal of H3K4me1...-dead Staphylococcus aureus Cas9 (Sa-Cas) and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1...

...the authors used a p53 response-element NanoLuc reporter to show that the mutants abolished transcriptional...This work highlights HiBiT tagging as a sensitive reporter for tracking proteomic cargo flow via EVs. Find...protein analysis products. You can find plasmids deposited by Promega scientists here.  References and...., & Wood, K. V. (2012). Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone...carcinoma in human tissue and in vitro. Oncology Reports, 50(5). https://doi.org/10.3892/or.2023.8631 Tsien...

... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. Here's what... intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the Loren ...license. While the GENIE team tested and deposited versions with both PDGFR and NGR fusions for membrane...optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the control of one of seven enhancers...interneurons in mice. A) AAV expression of dTomato reporter (magenta) under the control of the BiPVe3 enhancer...

... fans of all our depositors here at Addgene — your contributions make the repository what it is. So we...we’re thrilled this week to congratulate depositor David Baker, who was awarded the Nobel Prize in Chemistry...pressures during protein evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along with ...

... SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single molecules of RNA or...transcription factors with GFP Kevin White’s lab recently deposited a set of CRISPR plasmids to tag human transcription...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

...Our plasmid repository succeeds because of our amazing community. The trust of our depositors to allow us...more scientists outside of the US now use the repository – about 50% of plasmids are shipped to labs outside...manage our website, and continue to improve the repository. We love being able to provide a useful (and ...

... to all articles, Anurag Acharya and colleagues report, the percentage of citations to articles in non-elite...publications. (As an interesting aside, Nature recently reported that the most cited work in history is a 1951 ... the best way to measure impact? As that Nature report also noted, papers detailing Nobel Prize-winning... for the impact of research" (2013) Scientific Reports 3, Article number: 1649 doi:10.1038/srep01649 ...

...We’ve dug into the data from our repository to find our most requested plasmid in 2015. In line with ...that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR...fantastic research tool has been requested from the repository nearly 1,000 times in 2015! If you haven’t already... labs all around the world. We thank our many depositors for making these reagents available to the research...

...visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an ...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...References 1. Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms...Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology Plasmids with Addgene ...

...after they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble...now have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene...with scientists, but 2012 was marked by increased deposits (and requests) of biosensors, optogenetics, and...

...AAV composed of an AAV plasmid in the Addgene repository and their choice of five different serotypes....ordering high-quality materials from the Addgene repository. Read on to learn more.  What will be available...available?  Over 3000 AAV plasmids in the Addgene repository will be available for Packaged on Request service...ensuring we can maintain high standards for both our depositors and requestors.  Requesting Plasmids available...

...using the BE4max architecture. The new editors reported in this study have ~10-100-fold lower average ... gRNA multiplex. Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

...sequencing results to perform quality control on deposited plasmids. We’ve also talked about how our new .... This saves us time as we are often analyzing deposits with many plasmids that contain similar features...match sequence information contributed by the depositor, published backbone sequences, and any known sequences...analyze sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps...

...detects. This change then generates a reporter signal. Reporter signals may be electrochemical or light-based...light-based, with luminescent and fluorescent reporters being especially popular. We’ll give you an introduction...glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor...