We narrowed to 243 results for: grn

1. X-CHIME: Context Dependent Germline Knockout in Immune Cells

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   ...stem cells with a lentiviral vector containing a gRNA. These transduced stem cells can be implanted in...: Validating Your Genome Edit How to Design Your gRNA for CRISPR Genome Editing CRISPR Cheat Sheat Resources...

2. CASTing Off for New Shores in Human Genome Editing

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   ...system identifies and binds a genomic target using a gRNA, and an affiliated TniQ protein recruits transposition...integrate large sequences into genomic DNA downstream of gRNA-specified sites. Created with BioRender.com. ...

3. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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   ...including the cell line/tissue type, enzyme system, sgRNA library and other details relevant to screen result...gene-level scores provided by authors rather than sgRNA-level sequence read scores. The original supplemental...

4. Hot Plasmids and Viral Preps - July 2021

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   ...Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene.  CRISPR-Act3.0, developed by Yiping Qi's...Shirley Liu's lab targets all mouse genes with 10 gRNAs per gene. The guides are optimized to maximize on-target...

5. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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   ...This ‘Caspex’ construct is guided by a pre-defined sgRNA sequence to a specific genomic locus and labels ... and targeted to a sequence of interest with an sgRNA expressed from a separate plasmid. When cells are...

6. 10 Great Guest Posts We're Thankful For

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   ...use CombiGEM to barcode small libraries of CRISPR gRNAs to use for screening experiments. Get the full details...modular RNA binding proteins, dCas9, and a modified gRNA to direct a variety of customizable functions to...

7. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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   ... plasmid or in vitro transcribed, and changes in gRNA secondary structure can affect editing efficiency...will represent a key advantage over Cas9, for which gRNA sequence can greatly affect editing efficiency. ...

8. Another Pathway into Cells: iTOP

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   ...iTOP efficiently took up both Cas9 protein and a gRNA (yes, iTOP can also be used for RNA transduction...of iTOP. iTOP can be used to transduce Cas9 and a gRNA, which subsequently enter the nucleus and cleave...

9. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...lentiviral expression vector, which contains an sgRNA insert site and a reporter for tracing the engineered...editing tool on the immune system, the group inserted sgRNAs targeting various innate and adaptive immune genes...

10. Targeted Mutagenesis with EvolvR

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    ...for targeted mutagenesis of a site of interest. A gRNA is used to direct the PolI3M-nCas9 complex to a ...window also increased the likelihood that a single gRNA would introduce more than one mutation near the ...

11. Viral Vectors 101: Viral Applications

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    ...shRNA sequence specific to your gene’s mRNA or a sgRNA targeting an exon in your gene of interest, for ...with many applications. These libraries include sgRNAs targeting the entire genome, overexpression of ...

12. Plasmids 101: The protein expression toolbox

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    ...your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation... to a dead Cas9. The system is led, via a custom gRNA, to your gene of interest to temporarily increase...

13. Plasmids 101: Modular Cloning

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    ...many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed gRNA systems...

14. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ...(SAM) Description SAM uses specially engineered sgRNAs to increase transcription. This is done through...than relying on a two-component system dependent on gRNA design (SAM) or peptide design (SunTag). This streamlines...

15. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    ...cellular DNA and RNA dependent or independent of the gRNA used (Gaudelli et al., 2017). The authors tested...al., 2020) was able to reduce off-target RNA and gRNA-dependent DNA editing while maintaining on-target...

16. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ... Type Choose from: Encodes one insert, encodes gRNA/shRNA, or empty backbone. Purpose Answer “...Citing Addgene Plasmids Searchable and Sortable gRNAs for Your Next CRISPR Experiment Suggest A Plasmid...

17. Hot Plasmids and Viral Preps - May 2021

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    ... system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool...cerevisiae genes with 6-12 guides per gene where gRNA expression is induced by anhydrous tetracycline....

18. Analyzing CRISPR Editing Results with ICE from Synthego

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    ...those generated by multiplexed delivery of multiple sgRNAs. Since multiplexing is commonly used to create ...helps the researcher see which of the multiplexed sgRNA was involved in a particular edit, and which type...

19. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...check that your tracrRNA and crRNA (or synthetic gRNA) are derived from the same species. Expanding the...Guide Browse All CRISPR Plasmids Find Validated gRNAs ...

20. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ... the BFP variant, a BE4am-Cas9 base editor, and sgRNA vectors for the BFP-to-GFP conversion and to target...cytidine base editor. The lab then introduced an sgRNA targeting the GFPACG to the cells. As expected, ...