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  1. DNA Purification Without a Kit

    Type
    Blog Post
    ...Purification Method Using Glass Syringe Filters. PloS one. https://doi.org/10.1371/journal.pone.0007750  Vogelstein... of the United States of America, 76 2, 615-9 . https://doi.org/10.1073/pnas.76.2.615  Zhou, Y., Zhang...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou, Y., Mason...and microbes in under 30 seconds. PLoS biology. https://doi.org/10.1371/journal.pbio.2003916...

  2. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Kingdom of Saudi Arabia. Bioinformation, 14, 53–59. https://doi.org/10.6026/97320630014053 Barlow-Anacker,...increased B cell activation. PLOS ONE, 12, e0180664. https://doi.org/10.1371/journal.pone.0180664 Boix, F.,...recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23 Jhunjhunwala, S.,...Sterile Implant Materials. PloS One, 10, e0137550. https://doi.org/10.1371/journal.pone.0137550 More resources...

  3. Hot Plasmids - August 2020

    Type
    Blog Post
    ... Ting. Sanchez MI et al., Nat methods. 2019. https://doi.org/10.1038/s41592-019-0665-7 Optimizing ... Addgene! Chertkova, AO, et al., bioRxiv. 2020. https://doi.org/10.1101/160374 Nanobodies- there's a ...purification. Iwaki et al., Protein Expr Purif, 2020. https://doi.org/10.1016/j.pep.2020.105607 A new multiplexed...plasmids! Hazelbaker et al., Sci Reports. 2020. https://doi.org/10.1038/s41598-020-57500-1 The CRISPR...

  4. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...Resources http://www.businessinsider.com/how-to-get-the-most-out-of-job-fairs-2014-3 https://www.forbes.com...surprising-rules-you-should-know-regarding-linkedin-etiquette/5/#4050d70b1e9c https://www.linkedin.com/pulse/20140417174121-34888774...-don-ts Resources for getting into grad school: https://www.gradschools.com/get-informed/grad-school-roadmap...

  5. Lentiviral Vector Guide

    Type
    Guide
    ...Pseudotyping. Current Gene Therapy , 5 (4), 387–398. https://doi.org/10.2174/1566523054546224 PMID: 16101513...System. Journal of Virology , 72 (11), 8463–8471. https://doi.org/10.1128/jvi.72.11.8463-8471.1998 PMID:...of lentiviral vectors. Viruses , 3 (2), 132–159. https://doi.org/10.3390/v3020132 PMID: 22049307 Ghaleh.... Biomedicine & Pharmacotherapy , 128 , 110276. https://doi.org/10.1016/j.biopha.2020.110276 PMID: 32502836... Methods & Clinical Development , 21 , 451–465. https://doi.org/10.1016/j.omtm.2021.03.018 PMID: 33981779... Methods & Clinical Development , 20 , 792–800. https://doi.org/10.1016/j.omtm.2020.10.012 PMID: 33768124...Integration Targeting. PLoS ONE , 2 (12), e1340. https://doi.org/10.1371/journal.pone.0001340 PMID: 18092005...

  6. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... Acids Research Volume 42, Issue 22, Page e168. https://doi.org/10.1093/nar/gku936 Eva K. Brinkman, Arne...Nucleic Acids Research Volume 46, Issue 10, Page e58. https://doi.org/10.1093/nar/gky164 Cancellieri S, Canver...genome editing. (2019) Bioinformatics 36:2001–2008. https://doi.org/10.1093/bioinformatics/btz867 Haeussler... selection tool CRISPOR. (2016) Genome Biol 17: https://doi.org/10.1186/s13059-016-1012-2 Find more CRISPR...

  7. Adenovirus Guide

    Type
    Guide
    ...strategies . Current Gene Therapy, 11 (4), 307–320. https://doi.org/10.2174/156652311796150372 (Link opens...Signal Transduction and Targeted Therapy, 6 (1), 53. https://doi.org/10.1038/s41392-021-00487-6 (Link opens...virus vaccine . Scientific Reports, 10 (1), 3613. https://doi.org/10.1038/s41598-020-60238-5 (Link opens...Vaccines & Immunotherapeutics, 13 (12), 3020–3032. https://doi.org/10.1080/21645515.2017.1383575 (Link opens...challenge . Journal of Virology, 85 (9), 4222–4233. https://doi.org/10.1128/JVI.02407-10 (Link opens in a ... Expert Review of Vaccines, 14 (10), 1347–1357. https://doi.org/10.1586/14760584.2015.1077122 (Link opens...the United States of America, 95 (5), 2509–2514. https://doi.org/10.1073/pnas.95.5.2509 (Link opens in ...

  8. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...Psychological and Personality Science 11:647–657 . https://doi.org/10.1177/1948550619882032 Chrousos GP, ...syndrome threatens diversity. Science 367:749.2-750 . https://doi.org/10.1126/science.aba8039 Cordova FA (2016...2016) Embrace uncertainty. Science 351:994–994 . https://doi.org/10.1126/science.351.6276.994 Moore E (...Here’s how I built a network of mentors. Science. https://doi.org/10.1126/science.caredit.abi6939 Additional...

  9. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...nervous system . Molecular Therapy, 10 (2), 302–317. https://doi.org/10.1016/j.ymthe.2004.05.024 (Link opens...systems . Nature Neuroscience, 20 (8), 1172–1179. https://doi.org/10.1038/nn.4593 (Link opens in a new window...comparison . Molecular Therapy, 4 (4), 383–391. https://doi.org/10.1006/mthe.2001.0456 (Link opens in ...therapy . Current Gene Therapy, 3 (4), 281–304. https://doi.org/10.2174/1566523034578285 (Link opens in...production . Journal of Virology, 77 (8), 4881–4887. https://doi.org/10.1128/jvi.77.8.4881-4887.2003 (Link ...transfer . Pharmaceutical Research, 25 (3), 489–499. https://doi.org/10.1007/s11095-007-9431-0 (Link opens ...Cytokine & Growth Factor Reviews, 80 , 109–120. https://doi.org/10.1016/j.cytogfr.2024.09.003 (Link opens...

  10. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...immune system. Nature Immunology, 25(1), Article 1. https://doi.org/10.1038/s41590-023-01689-6 LaFleur, M....tumor immunity. Nature Immunology 20, 1335–1347. https://doi.org/10.1038/s41590-019-0480-4 LaFleur, M. ...immune system. Nature Communications, 10(1), 1668. https://doi.org/10.1038/s41467-019-09656-2 More resources...

  11. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ... of Norepinephrine. Neuron, 102(4), 745-761.e8. https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki...norepinephrine in vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038/s41592-023-01959-z Mirdita, M....accessible to all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038/s41592-022-01488-1 More resources...

  12. Hot Plasmids - October 2022

    Type
    Blog Post
    ...2022). https://doi.org/10.1038/s41593-022-01094-6. (This work was also shared as a preprint: https://doi.org... microscopy. Cell, 18, 3408 - 3425.e29. (2022). https://doi.org/10.1016/j.cell.2022.07.013.   Novel...

  13. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...adaptive immune responses. Cell Res 20, 34–50 (2010). https://doi.org/10.1038/cr.2009.139 Janda, A., Bowen, ...Structure and Function. Frontiers in Microbiology, 7. https://www.frontiersin.org/articles/10.3389/fmicb.2016.00022...specificity. Trends in Immunology, 29(2), 91–97. https://doi.org/10.1016/j.it.2007.11.004 Xu Cao, et al...

  14. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...analysis in Drosophila. Nature Methods 9:47–55 . https://doi.org/10.1038/nmeth.1800 Isaacman-Beck J, Paik...manipulate precise proportions of cells. bioRxiv 788679; https://doi.org/10.1101/788679 Lin R, Wang R, Yuan J, ...of single neurons. Nature Methods 15:1033–1036 . https://doi.org/10.1038/s41592-018-0184-y Additional ...

  15. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...Perspective and Future Outlook. Front Immunol 8:1589. https://doi.org/10.3389/fimmu.2017.01589 Jin B, Odongo...Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https://doi.org/10.3390/ijms24065994 Xu J, Xu K, Jung ...neutralize SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586-021-03676-z  More resources...

  16. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ... Vector for Gene Therapy. BioDrugs 31:317–334 . https://doi.org/10.1007/s40259-017-0234-5  Büning H, Srivastava...Therapy - Methods & Clinical Development 12:248–265 . https://doi.org/10.1016/j.omtm.2019.01.008  Maurer AC,...and Transduction. Human Gene Therapy 31:499–511 . https://doi.org/10.1089/hum.2020.069  Additional resources...

  17. Plan Your Experiment

    Type
    Guide
    ... Methods in Molecular Biology , 1239 , 197–217. https://doi.org/10.1007/978-1-4939-1862-1_10 PMID: 25408407...Genetic Screens. Cell , 167 (7), 1853–1866.e17. https://doi.org/10.1016/j.cell.2016.11.038 PMID: 27984732...mammalian cells. Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/s41596-022-00724-4 PMID: 35941224...repression and activation. Cell , 159 (3), 647–661. https://doi.org/10.1016/j.cell.2014.09.029 PMID: 25307932...editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID: 25307932...experiments. Nature Biotechnology , 38 (7), 813–823. https://doi.org/10.1038/s41587-020-0490-7 PMID: 29305085...genome editing. Scientific Reports , 5 , 11315. https://doi.org/10.1038/srep11315 PMID: 26066060 Zukher...

  18. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...multiplex immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554/elife.43322 Bandrowski, AE, Martone...Transparency of Experimental Methods. Neuron 90(3):434-6. https://doi.org/10.1016/j.neuron.2016.04.030    Bradbury... antibodies used in research. Nature 518:27–29. https://doi.org/10.1038/518027a  Resources We would also...
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