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Showing: 121 - 140 of 219 results
  1. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...Application to DNA Assembly. ACS Synthetic Biology. https://doi.org/10.1021/acssynbio.8b00333 Pryor, J. M....Design. ACS Synthetic Biology, 11(6), 2036–2042. https://doi.org/10.1021/acssynbio.1c00525 Pryor, J. M....data-optimized assembly design. PLOS ONE, 15(9), e0238592. https://doi.org/10.1371/journal.pone.0238592    ...
  2. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    .... 46, Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813 Erickson, P...Methods in Cell Biology (pp. 283–310). Elsevier. https://doi.org/10.1016/s0091-679x(08)60255-1  Manning..., p. e38313). Public Library of Science (PLoS). https://doi.org/10.1371/journal.pone.0038313 More resources...
  3. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...Vector Delivery. Molecular Therapy 12:744–753 . https://doi.org/10.1016/j.ymthe.2005.06.003 Guerin K, ...Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277 Lecomte E, Tournaire...Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Wright JF, Zelenaia...
  4. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...organization deciphering. Molecular Cytogenetics, 11. https://doi.org/10.1186/s13039-018-0368-2  Liu X, Zhang...by Biotinylated dCas9. Cell 170:1028–1043.e19 . https://doi.org/10.1016/j.cell.2017.08.003 Liu, X., Chen...chromatin by biotinylated dCas9. Genome Biology, 21. https://doi.org/10.1186/s13059-020-01973-w Additional ...
  5. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...Journal of Clinical Microbiology, 23(5), 959–961. https://doi.org/10.1128/jcm.23.5.959-961.1986 Lobstein...its cytoplasm. Microbial Cell Factories, 11, 56. https://doi.org/10.1186/1475-2859-11-56 Trastoy, M. O....cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on Addgene.org...
  6. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128/aem.04023-14 Li Q, Sun B, Chen...Escherichia coli. Acta Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 Zhang Y, Sun X, Wang...CRISPR-Associated Transposases. ACS Synth Biol 9:1998–2008. https://doi.org/10.1021/acssynbio.0c00073   Additional...
  7. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/978-1-4939-8935-5_26 Stuurman ...Introduction to Fluorescence Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy...validation of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995 Additional Resources...
  8. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... Tet-On and Tet-Off systems: second generation. https://www.takarabio.com/products/gene-function/tet-inducible-expression-systems...in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750-6-43Kaczmarczyk A, Vorholt...overexpression transgenes. Sci Rep 12, 3835 (2022). https://doi.org/10.1038/s41598-022-07852-7 De Boer, Herman...
  9. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...Mammalian Cell Culture. Anal Chem 84:4227–4232 . https://doi.org/10.1021/ac2033112 Ligasová A, Vydržalová...Mycoplasmas Using Fluorescence Microscopy. Cells 8:1510 . https://doi.org/10.3390/cells8121510 Sung H, Kang SH, ...Cell Lines. In Vitro Cell Dev Biol Anim 38:86 . https://doi.org/10.1290/1071-2690(2002)038<0086:caeomi...
  10. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    .... Review of Scientific Instruments 43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow...Overview. Current Protocols in Immunology 120: . https://doi.org/10.1002/cpim.40 Picot J, Guerin CL, Le...recent instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616-011-9415-0 Additional resources...
  11. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ... #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com/OOrwo9feyJ — BTC...Remind them you care!#thanks #health #mentalhealth https://t.co/Td2M6AOpuW pic.twitter.com/ofLI4eD8Zs — Microbigals...
  12. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ... human disease.     Sun, et al. bioRxiv. 2021. https://doi.org/10.1101/2020.05.04.076067    Novel fluorescent...Chesterfield et al., ACS Synthetic Biology 2020. https://pubs.acs.org/doi/10.1021/acssynbio.0c00192   ...
  13. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...Review of Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-010814-124803...Chemogenetics. In Methods in Enzymology (pp. 181–196). https://doi.org/10.1016/bs.mie.2018.01.022 Additional ...
  14. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...Protocols in Protein Science. John Wiley & Sons, Inc. https://doi.org/10.1002/0471140864 Fox, J. D., & Waugh...Protocols (Vol. 205, pp. 99–118). Humana Press. https://doi.org/10.1385/1-59259-301-1:99 Resources on ...
  15. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...eukaryotic proteome? Open Biol. (2019) 9:180241. DOI: https://doi.org/10.1080/rsob.180241 Vanni, C., et al.,...coding sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene...
  16. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ... GPCR transducerome. Nat Chem Biol 16:841–849 . https://doi.org/10.1038/s41589-020-0535-8 Takeda S, Kadowaki...human genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional...
  17. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...      Moore et al., ACS Synthetic Biology 202. https://doi.org/10.1021/acssynbio.0c00581   New tool ...Brookhouser, N., et al. BMC Biol 18, 193 (2020). https://doi.org/10.1186/s12915-020-00929-7    The CRISPR...
Showing: 121 - 140 of 219 results