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TypeBlog Post... Ting. Sanchez MI et al., Nat methods. 2019. https://doi.org/10.1038/s41592-019-0665-7 Optimizing ... Addgene! Chertkova, AO, et al., bioRxiv. 2020. https://doi.org/10.1101/160374 Nanobodies- there's a ...purification. Iwaki et al., Protein Expr Purif, 2020. https://doi.org/10.1016/j.pep.2020.105607 A new multiplexed...plasmids! Hazelbaker et al., Sci Reports. 2020. https://doi.org/10.1038/s41598-020-57500-1 The CRISPR...
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Antibodies 101: Reading a Flow Plot
TypeBlog Post...Kingdom of Saudi Arabia. Bioinformation, 14, 53–59. https://doi.org/10.6026/97320630014053 Barlow-Anacker,...increased B cell activation. PLOS ONE, 12, e0180664. https://doi.org/10.1371/journal.pone.0180664 Boix, F.,...recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23 Jhunjhunwala, S.,...Sterile Implant Materials. PloS One, 10, e0137550. https://doi.org/10.1371/journal.pone.0137550 More resources... -
CRISPR 101: Validating Your Genome Edit
TypeBlog Post... Acids Research Volume 42, Issue 22, Page e168. https://doi.org/10.1093/nar/gku936 Eva K. Brinkman, Arne...Nucleic Acids Research Volume 46, Issue 10, Page e58. https://doi.org/10.1093/nar/gky164 Cancellieri S, Canver...genome editing. (2019) Bioinformatics 36:2001–2008. https://doi.org/10.1093/bioinformatics/btz867 Haeussler... selection tool CRISPOR. (2016) Genome Biol 17: https://doi.org/10.1186/s13059-016-1012-2 Find more CRISPR... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...Psychological and Personality Science 11:647–657 . https://doi.org/10.1177/1948550619882032 Chrousos GP, ...syndrome threatens diversity. Science 367:749.2-750 . https://doi.org/10.1126/science.aba8039 Cordova FA (2016...2016) Embrace uncertainty. Science 351:994–994 . https://doi.org/10.1126/science.351.6276.994 Moore E (...Here’s how I built a network of mentors. Science. https://doi.org/10.1126/science.caredit.abi6939 Additional... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post...immune system. Nature Immunology, 25(1), Article 1. https://doi.org/10.1038/s41590-023-01689-6 LaFleur, M....tumor immunity. Nature Immunology 20, 1335–1347. https://doi.org/10.1038/s41590-019-0480-4 LaFleur, M. ...immune system. Nature Communications, 10(1), 1668. https://doi.org/10.1038/s41467-019-09656-2 More resources... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post... of Norepinephrine. Neuron, 102(4), 745-761.e8. https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki...norepinephrine in vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038/s41592-023-01959-z Mirdita, M....accessible to all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038/s41592-022-01488-1 More resources... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...analysis in Drosophila. Nature Methods 9:47–55 . https://doi.org/10.1038/nmeth.1800 Isaacman-Beck J, Paik...manipulate precise proportions of cells. bioRxiv 788679; https://doi.org/10.1101/788679 Lin R, Wang R, Yuan J, ...of single neurons. Nature Methods 15:1033–1036 . https://doi.org/10.1038/s41592-018-0184-y Additional ... -
Antibodies 101: Fc Effector Functions
TypeBlog Post...adaptive immune responses. Cell Res 20, 34–50 (2010). https://doi.org/10.1038/cr.2009.139 Janda, A., Bowen, ...Structure and Function. Frontiers in Microbiology, 7. https://www.frontiersin.org/articles/10.3389/fmicb.2016.00022...specificity. Trends in Immunology, 29(2), 91–97. https://doi.org/10.1016/j.it.2007.11.004 Xu Cao, et al... -
Year of the Camelid: Antibody Style
TypeBlog Post...Perspective and Future Outlook. Front Immunol 8:1589. https://doi.org/10.3389/fimmu.2017.01589 Jin B, Odongo...Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https://doi.org/10.3390/ijms24065994 Xu J, Xu K, Jung ...neutralize SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586-021-03676-z More resources... -
Hot Plasmids - October 2022
TypeBlog Post...2022). https://doi.org/10.1038/s41593-022-01094-6. (This work was also shared as a preprint: https://doi.org... microscopy. Cell, 18, 3408 - 3425.e29. (2022). https://doi.org/10.1016/j.cell.2022.07.013. Novel... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post... Vector for Gene Therapy. BioDrugs 31:317–334 . https://doi.org/10.1007/s40259-017-0234-5 Büning H, Srivastava...Therapy - Methods & Clinical Development 12:248–265 . https://doi.org/10.1016/j.omtm.2019.01.008 Maurer AC,...and Transduction. Human Gene Therapy 31:499–511 . https://doi.org/10.1089/hum.2020.069 Additional resources... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...sparse labeling Isaacman-Beck et al., bioRxiv 2019. https://doi.org/10.1101/788679 A destabilized GFP reporter...activity here Diessl et al., Microbial Cell, 2020. https://dx.doi.org/10.15698%2Fmic2020.04.713 Truncated...expressing tTALE-FP Shin et al., Sci. Rep. 2019. https://doi.org/10.1038/s41598-019-53722-0 ... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Maynard LH, Smith...Settings. Human Gene Therapy Methods 30:195–205 . https://doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha... Preparations. Molecular Therapy 25:1375–1386 . https://doi.org/10.1016/j.ymthe.2017.04.001 Additional... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog Post...multiplex immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554/elife.43322 Bandrowski, AE, Martone...Transparency of Experimental Methods. Neuron 90(3):434-6. https://doi.org/10.1016/j.neuron.2016.04.030 Bradbury... antibodies used in research. Nature 518:27–29. https://doi.org/10.1038/518027a Resources We would also... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post...neutralization by convalescent plasma. Nature 593:142–146. https://doi.org/10.1038/s41586-021-03471-w Garcia-Beltran...disease severity and survival. Cell 184:476-488.e11. https://doi.org/10.1016/j.cell.2020.12.015 Garcia-Beltran...vaccine-induced humoral immunity. Cell 184:2372-2383.e9. https://doi.org/10.1016/j.cell.2021.03.013 Additional ... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post...antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a Bradbury AM, Plückthun...validate recombinants once. Nature 520:295–295 . https://doi.org/10.1038/520295b Bradbury ARM, Trinklein... functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080/19420862.2018.1445456 Additional... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post...Antibody Staining. J Histochem Cytochem 62:197–204. https://doi.org/10.1369/0022155413511620 Lu QL, Partridge...Tissue Sections. J Histochem Cytochem 46:977–983. https://doi.org/10.1177/002215549804600813 Tuson JR, ...Histochem Cytochem : Off J Histochem Soc 38:923–926. https://doi.org/10.1177/38.7.2355173 Additional resources... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...in Medicine & Biology Society (EMBC), 4322–4325. https://doi.org/10.1109/EMBC44109.2020.9175600 Park, S...interfaces. Chemical Society Reviews, 48(6), 1826–1852. https://doi.org/10.1039/C8CS00710A Sahasrabudhe, A., Rupprecht...brain neural circuits. Nature Biotechnology, 1–13. https://doi.org/10.1038/s41587-023-01833-5 Resources on... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post...have they done to and for us? BMC Biology, 15, 91. https://doi.org/10.1186/s12915-017-0433-z. PMID: 29052511...Virus Replication. Essential Human Virology, 49. https://doi.org/10.1016/B978-0-12-800947-5.00004-1 Taylor...(pp. 23–40). Springer International Publishing. https://doi.org/10.1007/978-3-319-07758-1_2 More resources... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog Post...-authored paper. PLoS Comput Biol 14:e1006508 . https://doi.org/10.1371/journal.pcbi.1006508 Vicens Q,...Successful Collaboration. PLoS Comput Biol 3:e44 . https://doi.org/10.1371/journal.pcbi.0030044 Wuchty S,...Production of Knowledge. Science 316:1036–1039 . https://doi.org/10.1126/science.1136099 ...