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  1. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...transposases. Nature Protocols, 19(3), 752–790. https://doi.org/10.1038/s41596-023-00927-3 Klompe, S. ...RNA-guided DNA integration. Nature, 571(7764), 219–225. https://doi.org/10.1038/s41586-019-1323-z Peters, J. E...United States of America, 114(35), E7358–E7366. https://doi.org/10.1073/pnas.1709035114 Witte, I. P., ...CRISPR-associated transposase. Science, 388(6748), eadt5199. https://doi.org/10.1126/science.adt5199 Additional Resources...

  2. Hot Plasmids - October 2022

    Type
    Blog Post
    ...2022). https://doi.org/10.1038/s41593-022-01094-6. (This work was also shared as a preprint: https://doi.org... microscopy. Cell, 18, 3408 - 3425.e29. (2022). https://doi.org/10.1016/j.cell.2022.07.013.   Novel...Molecular and Cellular Biology, 5(12), 3610–3616. doi: https://doi.org/10.1128/mcb.5.12.3610-3616.1985.    ...

  3. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...Addgene: Plasmids for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described...  Zalatan et al. Cell  (2015) can be found here: https://www.addgene.org/browse/article/9593/ Plasmids...described in Qi et al. Cell  (2013) can be found here: https://www.addgene.org/browse/article/6441/ Resources...characterized aptamers and supporting publication: http://www.freebase.com/base/aptamer Cruz-Toledo, J...

  4. Hot Plasmids - February 2022

    Type
    Blog Post
    ...Genes Cells. 2021. https://doi.org/10.1111/gtc.12893, Zhao et al. Nat Comm. 2019. https://doi.org/10.1038...domains.  Santilli, R.T., et al. Sci Rep (2021). https://doi.org/10.1038/s41598-021-96051-x Engineered...therapy.  Xiaoshu X, et al. Molecular Cell. 2021. https://doi.org/10.1016/j.molcel.2021.08.008. ...

  5. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...human GPCRome. Nat Struct Mol Biol 22:362–369 . https://doi.org/10.1038/nsmb.3014 Paek J, Kalocsay M, ...Peroxidase-Catalyzed Proximity Labeling. Cell 169:338-349.e11 . https://doi.org/10.1016/j.cell.2017.03.028 Roth BL (2016...DREADDs for Neuroscientists. Neuron 89:683–694 . https://doi.org/10.1016/j.neuron.2016.01.040 Takeda S,...human genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional...

  6. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...the National Academy of Sciences 104:5163–5168 . https://doi.org/10.1073/pnas.0700293104 Field J, Nikawa...epitope addition method. Mol Cell Biol 8:2159–2165 . https://doi.org/10.1128/mcb.8.5.2159 Galvan A, Raper J...DREADD s in monkeys. Eur J Neurosci 50:2801–2813 . https://doi.org/10.1111/ejn.14429 Kimple ME, Brill AL,...Purification. Current Protocols in Protein Science 73: . https://doi.org/10.1002/0471140864.ps0909s73 Additional...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    ...Biophysica Acta (BBA) - Enzymology 341:484–488. https://doi.org/10.1016/0005-2744(74)90240-x Chirgwin ...enriched in ribonuclease. Biochemistry 18:5294–5299. https://doi.org/10.1021/bi00591a005 Chomczynski P, Sacchi...Extraction. Analytical Biochemistry 162:156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski...twenty-something years on. Nature Protocols 1:581–585. https://doi.org/10.1038/nprot.2006.83 Additional resources...

  8. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...and Environmental Microbiology, 67(8), 3440–3444. https://doi.org/10.1128/AEM.67.8.3440-3444.2001 Fogarty... in the human gut. Cell, 187(5), 1206-1222.e16. https://doi.org/10.1016/j.cell.2024.01.039 Garcillán-Barcia... Plasmid classifications. Plasmid, 126, 102684. https://doi.org/10.1016/j.plasmid.2023.102684 Shareck,...Critical Reviews in Biotechnology, 24(4), 155–208. https://doi.org/10.1080/07388550490904288 Additional Resources...

  9. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...hours.  Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org/doi/10.1021/acssynbio.9b00395 O’Brian...’Brian, SP, et al., Biotechnol J.  2014. https://pubmed.ncbi.nlm.nih.gov/24390935/ Hypercompact genome...from CRISPR-Casɸ. Pausch et al., Science 2020, https://pubmed.ncbi.nlm.nih.gov/32675376/ mGold, the ...molecules.  Lee, J. et al., Science Advances 2020. https://doi.org/10.1126/sciadv.abb7438 The CRISPR corner...

  10. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...extended imaging. Nature Communications, 16(1), 3241. https://doi.org/10.1038/s41467-025-58223-5.   TnpB, ... in Arabidopsis. Nature Plants, 11(5), 967–976. https://doi.org/10.1038/s41477-025-01989-9.   Make a...driver mutations. Cell Stem Cell, 32(4), 564–580.e6. https://doi.org/10.1016/j.stem.2025.01.012.  New AAV6...transsynaptic tracer. Nature Methods, 22(5), 1101–1111. https://doi.org/10.1038/s41592-025-02670-x.  ...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...HEK 293 cells. The FASEB Journal, 16(8), 869–871. https://doi.org/10.1096/fj.01-0995fje. Tan, E., Chin, ...Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.796991. Urabe, M., ...2 vectors. Human Gene Therapy, 13(16), 1935–43. https://doi.org/10.1089/10430340260355347. Vaughn, J. ...lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077....

  12. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...validation studies. Nat Commun. 13, 5495 (2022). doi: https://doi.org/10.1038/s41467-022-33246-4  Chung HK, ...degron. Nat Chem Biol. 2015 Sep;11(9):713-20. doi: https://doi.org/10.1038/nchembio.1869  Nabet B, Roberts...degradation. Nat Chem Biol. 2018 May;14(5):431-441. doi: https://doi.org/10.1038/s41589-018-0021-8  Holland AJ,...Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: https://doi.org/10.1073/pnas.1216880109  Resources on ...

  13. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... Cell. 2020. https://doi.org/10.1016/j.cell.2020.08.012  Starr et al Science. 2021. https://doi.org/10.1126...Preger et al., Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289     Novel protein...biosynthetic pathways.   Cheah et al., bioRxiv 2021. https://doi.org/10.1101/2021.01.30.428974   The CRISPR...

  14. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ... BLAST: ExPASy - http://www.expasy.org/genomics/sequence_alignment EMBL-EBI - http://www.ebi.ac.uk/services...services http://www.ebi.ac.uk/Tools/webservices/#multiple_sequence_alignment_msa http://www.ebi.ac.uk...

  15. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...artifacts and how to minimize them. FocalPlane. https://focalplane.biologists.com/2020/07/07/fixation-...Journal of Histochemistry & Cytochemistry 33:813–820. https://doi.org/10.1177/33.8.3894499    Im K, Mareninov...Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/978-1-4939-8935-5_26  Rolls G ...Nature of Fixatives in Histology. Leica Biosystems. https://www.leicabiosystems.com/us/knowledge-pathway/fixation-and-fixatives...

  16. DNA Purification Without a Kit

    Type
    Blog Post
    ...Purification Method Using Glass Syringe Filters. PloS one. https://doi.org/10.1371/journal.pone.0007750  Vogelstein... of the United States of America, 76 2, 615-9 . https://doi.org/10.1073/pnas.76.2.615  Zhou, Y., Zhang...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou, Y., Mason...and microbes in under 30 seconds. PLoS biology. https://doi.org/10.1371/journal.pbio.2003916...

  17. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Kingdom of Saudi Arabia. Bioinformation, 14, 53–59. https://doi.org/10.6026/97320630014053 Barlow-Anacker,...increased B cell activation. PLOS ONE, 12, e0180664. https://doi.org/10.1371/journal.pone.0180664 Boix, F.,...recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23 Jhunjhunwala, S.,...Sterile Implant Materials. PloS One, 10, e0137550. https://doi.org/10.1371/journal.pone.0137550 More resources...

  18. Hot Plasmids - August 2020

    Type
    Blog Post
    ... Ting. Sanchez MI et al., Nat methods. 2019. https://doi.org/10.1038/s41592-019-0665-7 Optimizing ... Addgene! Chertkova, AO, et al., bioRxiv. 2020. https://doi.org/10.1101/160374 Nanobodies- there's a ...purification. Iwaki et al., Protein Expr Purif, 2020. https://doi.org/10.1016/j.pep.2020.105607 A new multiplexed...plasmids! Hazelbaker et al., Sci Reports. 2020. https://doi.org/10.1038/s41598-020-57500-1 The CRISPR...

  19. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...Resources http://www.businessinsider.com/how-to-get-the-most-out-of-job-fairs-2014-3 https://www.forbes.com...surprising-rules-you-should-know-regarding-linkedin-etiquette/5/#4050d70b1e9c https://www.linkedin.com/pulse/20140417174121-34888774...-don-ts Resources for getting into grad school: https://www.gradschools.com/get-informed/grad-school-roadmap...

  20. Cutting Once Is Harder Than It Sounds: Your Guide to Minimizing Off-Target Effects

    Type
    Blog Post
    ...off-targets. Nature Communications, 14(1), 5746. https://doi.org/10.1038/s41467-023-41393-5 Kulcsár, P....G-extended sgRNAs. Nature Communications, 11(1), 1223. https://doi.org/10.1038/s41467-020-15021-5 Tálas, A., ...self-targeting sgRNAs. Nucleic Acids Research, 49(6), e31. https://doi.org/10.1093/nar/gkaa1220  Wang, D., Zhang,...deep learning. Nature Communications, 10, 4284. https://doi.org/10.1038/s41467-019-12281-8  Additional...
Showing: 121 - 140 of 285 results