We narrowed to 304 results for: human dat

...our recent blog post. Fatima Enam: Biosensors for human milk oligosaccharides Fatima Enam is currently a...developing fluorescent protein-based biosensors for Human Milk Oligosaccharides (HMOs). HMOs are thought to...development as a researcher and science communicator.  Update (7/17/2018): Check out Fatima's recent publication...

...Lin, Steven, et al. "Enhanced homology-directed human genome engineering by controlled timing of CRISPR... "Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins...nucleofection as well as the sequences of our validated editing reagents can be found on our plasmid pages...

...-editing field. It has the possibility to treat human genetic diseases, and several treatments are currently.... “Advances in CRISPR/Cas-based Gene Therapy in Human Genetic Diseases.” Theranostics. https://doi.org...originally written in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an Addgene co-op....” Cystic fibrosis may not be the most likely candidate for gene therapy, however, because the disease...

... Kroemer often functions as one of the “healthy human volunteers” in his studies! This was followed by...He also highlighted the UCSF Immunoprofiler - a human biopsy analysis platform focused on analysing immune... guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University! Cancer therapeutics ... potential to become an open-source pipeline and data repository for cancer researchers. Go open-source...

...novel form of AI that can learn new tasks and beat humans at the ancient Chinese game Go). In addition, there...disciplines to better understand neurobiology and human cognition. Developing better AI programs gives neurobiologists...really in all of biology) require large data sets. Whether these data sets include videos of behavior, many...

...optimized PE2 enzyme containing RT optimized for human codons, additional nuclear localization signals,...protection added to 3’ tail of pegRNA to prevent RNA degradation For more in-depth information on these PE tools...

.... et al. Isolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Meth... mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology 31, 822–826 (2013). ...genome editing. When it comes time to (hopefully) validate successful mutation of your favourite gene, there...guide you through the rough waters of mutation validation, such as this "CRISPR 101" post. Next-generation...While these methods paved the way for mutation validation, we found these assays frustrating to work with...post, we’ll introduce digital PCR as an emerging validation technology. Digital PCR has several advantages...editing workflow. Digital PCR comparison to other validation methods As mentioned, these assays have several...

...lentiviral prep. It's a human CRISPR inhibition library that inhibits over 18,000 human genes and is used for...quality control. For an estimate on availability date or to be notified when a particular catalog item...

...targeting the entire genome, overexpression of all human transcription factors, barcoded libraries to trace...features make them an ideal delivery system for human patients. Multiple viral-based gene therapies have...emerging viral applications and give you some recommendations for putting viruses to work. Viral applications...which target complementary mRNA sequence for degradation. Viral introduction of shRNA plasmids can induce...

...characterized these tools and validated their specificity in mice, non-human primates, and human tissue. Figure...recombinant antibodies against three of the six human glypican proteins (GPC1, GPC3, and GPC4). All IPI...we've already taken them for a test drive! We validated viral packaging and strong expression after inserting...from the antibody pages or in the Addgene Antibody Data Hub. Perhaps these antibodies are just the tools...

...activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR, Addgene gave... On the 5th day of CRISPR, Addgene gave to me: updated CRISPR101 blog posts!🎵 On the 4th day of CRISPR...High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects🎵 On the 1st ...

...effective in genome editing in both bacteria and human cells using various delivery methods, including ...RNAs, such as Cre mRNA or Cas9 mRNA and sgRNA, in human and mouse cells. They further develop a version ... AsCas12f1 plasmids   Selective endogenous encapsidation for cellular delivery of RNAs By Mike Lacy The... in an approach called Selective Endogenous eNcapsidation for cellular Delivery (SEND). They demonstrate...engineered pegRNAs (epegRNAs) protect the RNA from degradation by cellular exonucleases and broadly improve ...

...collection consists of human and murine solute channels, the team will be able to validate their findings in...in mammalian cells with both human and murine targets thus informing potential for success in a patient-mutation...vectors from Addgene, the lab will express these candidate genes in TS iPSCs to identify whether any rescue... use CRISPR plasmids to generate deletions of candidate genes in euploid iPSCs. “The BeHEARD Addgene reagents...

...designed by different academic labs, targeting mouse, human, Drosophila melanogaster, and T. gondii are available...contains most of the full genome knockout human and mouse libraries available through Addgene. This means...including other libraries. As of now I have focused on human and mouse SpCas9 knockout libraries, but there are...secondary, CRISPR screen could be used to validate the list of candidate genes. Green Listed, as of now, contains...groups and, as a consequence, which genes are good candidates for being involved in the studied phenotype. ... a massive amount of descriptive transcriptomics data. Custom CRISPR screens designed to target differentially...genes can be the next stage in making sense of such data. These targeted screens can allow a researcher to...

...infrequent genome integration at the AAVS1 locus in humans. Cap is a structural capsid protein that helps ...-293) during production. Fun fact: There are 57 human adenovirus types, but most vectors are based on ...used to package lab grown viruses. It can be intimidating at first to look at so many plasmid maps and...

...is important for therapeutic delivery, viruses, human disease, and much more! Resources and References...References Wang, I. X., Grunseich, C., Fox, J., et al., Human proteins that interact with RNA/DNA hybrids. Genome...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...

...Species of Gene or Insert Choose from: H. sapiens (human), M. musculus (mouse), R. norvegicus (rat), G. gallus...plasmid will produce anything dangerous or toxic to humans in bacteria, select “yes” in the drop-down and ...Table 1: Guidelines for the data fields in the deposit spreadsheet Data Field Description Plasmid...plasmids! We update the spreadsheet every so often and this ensures you are using our most up-to-date version...taken care of and, once we have all of your plasmid data, we’ll send you prepaid shipping materials (i.e....have the most involvement with Step 1, and, while data entry can be a chore, we’ve made it easy to send...plasmid information in a deposit spreadsheet. The data entry process On the Addgene home page, if you scroll...

...in a mouse may not work as well in a rat or non-human primate. Likewise, in vitro efficacy doesn’t always...Vector-Mediated Gene Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089...Expression mediated by three partial sequences of the human tyrosine hydroxylase promoter in vivo. Molecular...virus, a successful AAV experiment starts with validation and optimization. Although there are different...find it is usually faster and easier to do the validation first.  AAV-mediated gene expression can differ...inducing drug, so be sure to test for this when validating your viral vector. Titer Once you have selected...measurements. The number itself is determined through validation and optimization of your expression system for...

... an organism. These features make AAV ideal for human therapies and in vivo laboratory experiments ranging...medium during adeno-associated virus manufacturing." Human gene therapy 21.10 (2010): 1251-1257. PubMed PMID...we recommend checking the literature and our AAV data hub to see if others have used relevant viruses ... guest blogger Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik....

...secretion of growth promoting proteins, such as human growth factor, vascular endothelial growth factor...early tumor recurrence and poor clinical outcome in human breast cancers." The American journal of pathology...metabolism. Cancer cells promote oxidative stress leading to the degradation of one key enzyme (among others...