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We narrowed to 302 results for: human dat

Showing: 121 - 140 of 302 results

  1. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...health-related single nucleotide polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides a method to detect nucleic...targeted to a specific DNA sequence, such as the Human papilloma virus (HPV) genome, via a crRNA. An ssDNA-fluorescently...two similar types of HPV, HPV16 and HPV18, from human cells, at atomolar levels, within one hour. DETECTR...but is undergoing validation studies.     Jennifer Tsang contributed to updating this article. References...Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection...Doudna lab has used Cas12a’s non-specific ssDNA degradation to develop the method termed DNA Endonuclease...DETECTR harness the promiscuous cleavage and degradation of neighboring ssRNA and ssDNA by Cas13 and Cas12a...

  3. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...our recent blog post. Fatima Enam: Biosensors for human milk oligosaccharides Fatima Enam is currently a...developing fluorescent protein-based biosensors for Human Milk Oligosaccharides (HMOs). HMOs are thought to...development as a researcher and science communicator.  Update (7/17/2018): Check out Fatima's recent publication...

  4. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...Lin, Steven, et al. "Enhanced homology-directed human genome engineering by controlled timing of CRISPR... "Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins...nucleofection as well as the sequences of our validated editing reagents can be found on our plasmid pages...

  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Aebischer 11442 pBabe bleo human Frataxin FXN Friedreich ataxia Ronald Kahn 11443 pBS human Frataxin (myc tag)...11503 pBabe neo human Frataxin FXN Friedreich ataxia Ronald Kahn 11504 pBabe hygro human Frataxin FXN Friedreich...Sohail Tavazoie 51437 human Alpha-synuclein SNCA T7 Parkinson's Michael J Fox Foundation MJFF 51486 pET21a-alpha-synuclein...Parkinson's Michael J Fox Foundation MJFF 194247 pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA SNCA GFP CAG... Friedreich ataxia Ronald Kahn 11505 pBabe puro human Frataxin FXN Friedreich ataxia Ronald Kahn 11508...-Myc-B HTT Myc UbC Huntington's Baoji Xu 110758 Human Parkin (1-465) PRKN His, GST T7 Parkinson's David...CHMP2B mCherry CMV ALS Sanford Simon 115523 MSCV-human CSF-1Rwt-IRES-GFP CSF1R Hereditary diffuse leukoencephalopathy...

  6. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...-editing field. It has the possibility to treat human genetic diseases, and several treatments are currently.... “Advances in CRISPR/Cas-based Gene Therapy in Human Genetic Diseases.” Theranostics. https://doi.org...originally written in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an Addgene co-op....” Cystic fibrosis may not be the most likely candidate for gene therapy, however, because the disease...

  7. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...optimized PE2 enzyme containing RT optimized for human codons, additional nuclear localization signals,...protection added to 3’ tail of pegRNA to prevent RNA degradation For more in-depth information on these PE tools...

  8. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ... Kroemer often functions as one of the “healthy human volunteers” in his studies! This was followed by...He also highlighted the UCSF Immunoprofiler - a human biopsy analysis platform focused on analysing immune... guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University! Cancer therapeutics ... potential to become an open-source pipeline and data repository for cancer researchers. Go open-source...

  9. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...novel form of AI that can learn new tasks and beat humans at the ancient Chinese game Go). In addition, there...disciplines to better understand neurobiology and human cognition. Developing better AI programs gives neurobiologists...really in all of biology) require large data sets. Whether these data sets include videos of behavior, many...

  10. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    .... et al. Isolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Meth... mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology 31, 822–826 (2013). ...genome editing. When it comes time to (hopefully) validate successful mutation of your favourite gene, there...guide you through the rough waters of mutation validation, such as this "CRISPR 101" post. Next-generation...While these methods paved the way for mutation validation, we found these assays frustrating to work with...post, we’ll introduce digital PCR as an emerging validation technology. Digital PCR has several advantages...editing workflow. Digital PCR comparison to other validation methods As mentioned, these assays have several...

  11. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...lentiviral prep. It's a human CRISPR inhibition library that inhibits over 18,000 human genes and is used for...quality control. For an estimate on availability date or to be notified when a particular catalog item...

  12. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...targeting the entire genome, overexpression of all human transcription factors, barcoded libraries to trace...features make them an ideal delivery system for human patients. Multiple viral-based gene therapies have...emerging viral applications and give you some recommendations for putting viruses to work. Viral applications...which target complementary mRNA sequence for degradation. Viral introduction of shRNA plasmids can induce...

  13. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...characterized these tools and validated their specificity in mice, non-human primates, and human tissue. Figure...recombinant antibodies against three of the six human glypican proteins (GPC1, GPC3, and GPC4). All IPI...we've already taken them for a test drive! We validated viral packaging and strong expression after inserting...from the antibody pages or in the Addgene Antibody Data Hub. Perhaps these antibodies are just the tools...

  14. The 12 Days of CRISPR

    Type
    Blog Post
    ...activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR, Addgene gave... On the 5th day of CRISPR, Addgene gave to me: updated CRISPR101 blog posts!🎵 On the 4th day of CRISPR...High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects🎵 On the 1st ...

  15. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...effective in genome editing in both bacteria and human cells using various delivery methods, including ...RNAs, such as Cre mRNA or Cas9 mRNA and sgRNA, in human and mouse cells. They further develop a version ... AsCas12f1 plasmids   Selective endogenous encapsidation for cellular delivery of RNAs By Mike Lacy The... in an approach called Selective Endogenous eNcapsidation for cellular Delivery (SEND). They demonstrate...engineered pegRNAs (epegRNAs) protect the RNA from degradation by cellular exonucleases and broadly improve ...

  16. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...collection consists of human and murine solute channels, the team will be able to validate their findings in...in mammalian cells with both human and murine targets thus informing potential for success in a patient-mutation...vectors from Addgene, the lab will express these candidate genes in TS iPSCs to identify whether any rescue... use CRISPR plasmids to generate deletions of candidate genes in euploid iPSCs. “The BeHEARD Addgene reagents...

  17. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...designed by different academic labs, targeting mouse, human, Drosophila melanogaster, and T. gondii are available...contains most of the full genome knockout human and mouse libraries available through Addgene. This means...including other libraries. As of now I have focused on human and mouse SpCas9 knockout libraries, but there are...secondary, CRISPR screen could be used to validate the list of candidate genes. Green Listed, as of now, contains...groups and, as a consequence, which genes are good candidates for being involved in the studied phenotype. ... a massive amount of descriptive transcriptomics data. Custom CRISPR screens designed to target differentially...genes can be the next stage in making sense of such data. These targeted screens can allow a researcher to...

  18. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...infrequent genome integration at the AAVS1 locus in humans. Cap is a structural capsid protein that helps ...-293) during production. Fun fact: There are 57 human adenovirus types, but most vectors are based on ...used to package lab grown viruses. It can be intimidating at first to look at so many plasmid maps and...

  19. All in a Twist: dsRNA

    Type
    Blog Post
    ...is important for therapeutic delivery, viruses, human disease, and much more! Resources and References...References Wang, I. X., Grunseich, C., Fox, J., et al., Human proteins that interact with RNA/DNA hybrids. Genome...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...

  20. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...Species of Gene or Insert Choose from: H. sapiens (human), M. musculus (mouse), R. norvegicus (rat), G. gallus...plasmid will produce anything dangerous or toxic to humans in bacteria, select “yes” in the drop-down and ...Table 1: Guidelines for the data fields in the deposit spreadsheet Data Field Description Plasmid...plasmids! We update the spreadsheet every so often and this ensures you are using our most up-to-date version...taken care of and, once we have all of your plasmid data, we’ll send you prepaid shipping materials (i.e....have the most involvement with Step 1, and, while data entry can be a chore, we’ve made it easy to send...plasmid information in a deposit spreadsheet. The data entry process On the Addgene home page, if you scroll...
Showing: 121 - 140 of 302 results