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We narrowed to 126 results for: lenti crispr

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Showing: 121 - 126 of 126 results

  1. Guide to Using Pooled Libraries

    Type
    Guide
    ...Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Pooled CRISPR Libraries...protocol. Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse our...Examples of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-... virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells. The... plasmids have been depleted by selection. In a CRISPR screen, negative screens are often used to identify...cells of interest. With others, notably pooled lentiviral plasmid libraries, the plasmids must first be...using a pooled library. The process diverges for lentiviral of DNA-based screens. NGS = next-generation sequencing...

  2. Science Guides

    Type
    Guide
    ...Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems...background information on CRISPR and the basics for planning your first CRISPR experiment. Read More Optogenetics...links to the guides below. Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies...that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus...

  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Bonifacino 173579 Lenti-sgAtm#1/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ...Venus CMV Lewy body dementia Tiago Outeiro 89643 Lenti-sgAtm/Cre ATM U6 Ataxia telangiectasia Monte Winslow...Parkinson's Frank Soldner 180437 Px330-EGFP-LRRK2-CRISPR/Cas9 LRRK2 U6 Parkinson's Frank Soldner 180439 ...FusionRed CUP1 ALS Michihiko Kataoka 188460 pKDR-Lenti-CBh.6-SOD1 SOD1 CBh ALS Erik Dent 188572 Tet-off...LRRK2 His T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia...Cookson 13318 pLenti6-DEST PINK1-V5 G309D PINK1 V5 CMV Parkinson's Mark Cookson 13319 pLenti6-DEST PINK1-... Cookson 29412 pLenti6-DJ1-V5-E18N PARK7 V5 CMV Parkinson's Mark Cookson 29413 pLenti6-DJ1-V5-C106D PARK7...

  4. Molecular Biology Reference

    Type
    Guide
    ...genome. For more information on CRISPR check out our CRISPR Guide . CRISPR Plasmids , Cre-lox and Other ...editing is most commonly accomplished using CRISPR technology. CRISPR is composed of a DNA endonuclease and ...plasmids to create viral particles (most commonly lentiviral, retroviral, AAV, or adenoviral particles) that...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...Description Transactivator PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral Tet-On vector with blasticidin...rtTA-Advanced Mikhail Alexeyev 17492 pLenti CMV TetR Blast (716-1) Lentiviral Tet-On vector expressing TetR ...87360 TLCV2 Lentiviral vector for tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam ...KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for CRISPRa Emma Rawlins 72835 pMK243 (Tet-OsTIR1-PURO) Expresses...Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression of shRNA...2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design ...-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ... and Resources You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene...labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE) System Kit : A modular and ...system that can be used to construct complex lentiviruses for modification of mammalian cells with a single...
Showing: 121 - 126 of 126 results