We narrowed to 213 results for: pme i

...step “...proved to be quite easy to achieve…” - but I think we can all agree that it sounds less than ideal...9E10 antibody remains a trusty antibody partner; as I write this, there are close to 10,000 citations for...0092-8674(87)90086-9  Schüchner S, Behm C, Mudrak I, Ogris E (2020) The Myc tag monoclonal antibody 9E10... mysterious, microscopic world of cells. The development of antibodies as tools allowed us to detect and...

...writing up your research? What type of journal should I choose? Writing a research paper for publication in...to include in your manuscript. What information do I need to include in my paper? More and more journals...post was contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research.  With many changes ...figures and results. This is a relatively new development, and it allows reviewers and other researchers...You to Our Guest Blogger! Maaike Pols is a Developmental Editor for F1000Research, responsible for assessing...

..., and Dr. Angela Lin got me interested in TS when I was a newly independent faculty member studying sex...partners can regulate ion channels in the brain.  “I have a long standing interest in the biology of GPCRs...Deficiency Syndromes (CCDS) that present as global developmental delays or autism spectrum disorder. It is estimated...creatine deficiency, which is characterized by development delays, speech delays, seizures, and other symptoms... enhance our ability towards CTD therapeutic development,” says Iyer. Since the RESOLUTE plasmid collection...Though there are IL-36 receptor inhibitors in development, there’s still a lot of mysteries surrounding...in Turner syndrome Turner syndrome (TS) is a developmental disorder where one of the X chromosomes is partially...

...efficiencies in high-throughput libraries My labmates and I wanted to find a way to predict the efficiencies of... Weller, J., Peets, E. M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & ...Damodharan, T., Balázs, Z., Janjuha, S., Ioannidi, E. I., Böck, D., Steensel, B. van, Krauthammer, M., & Schwank... Allam. Continued training led to the recent development of PRIDICT2.0 (Mathis et al., 2024). The additional...

...of students and teachers in the U.S. and beyond. “I want to learn more about Synthetic Biology. Can you...students are asking. Teachers are also asking, “How do I engage my students in real-world lab activities? I...and collaboration. One module currently under development comes from the Caltech lab of Professor Richard... internships. A summer teacher professional development program that trains nearly 100 teachers each ...

...AAVs that have successful transduction in microglia? I have been using the AAV.PHP.eb for neuron, oligodendrocyte...immune suppression to allow AAV re-administration but I do not think the field has settled on ‘best practices...Re-administration is a challenge for AAV due to the development of neutralizing antibodies in animal models. ...design in remarkably creative ways. The continued development of more and smaller CRISPR tools is an exciting...

...the UN describes. In honor of the Camelid family, I wanted to take a moment to revisit some of the ways...discovery of heavy-chain only antibodies in a camel. And I suspect we will only continue to see their impact ...sdAbs come from? In the early years of sdAbs development, the process started with immunizing a camelid...

... for failure by delegating is a bad idea. How do I make sure it goes well? As with most managerial responsibilities...deadlines. Agree on a way to review project progress. I recommend putting a regular check-in meeting on the...Goals with Your Mentor through an Individual Development Plan Resources on the Addgene Website Are You...

...all over the world. When I was at the 22nd International C. elegans meeting, I was again reminded of the...zebrafish articles published there as well.   Worm Development Dynamics Database (WDDD) The WDDD aims to acquire...computational methods to better understand animal development (Kyoda et al., 2012).  C. elegans plasmid tools...PMC6576255. Kyoda, Koji, et al. "WDDD: worm developmental dynamics database." Nucleic acids research 41...conditional protein depletion in C. elegans." Development 142.24 (2015): 4374-4384. PubMed PMID: 26552885...

...Zetsche, B., Gootenberg, J., Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015... New Tool for CRISPR Genome Editing Which Cas9 Do I Choose for My CRISPR Experiment A Match Made in Heaven...Note: Cpf1 is also called Cas12a. There’s a new development for CRISPR-Cpf1 genome editing!  A recent paper...editing with CRISPR-Cpf1 is one of the latest developments in CRISPR technologies.  It’s a simple and effective...

...Addgene to accelerate ongoing COVID-19 research. “I hope that by sharing the plasmids other scientists... References and Resources References Cele S, Gazy I, Jackson L, Hwa S-H, Tegally H, Lustig G, Giandhari...link between COVID-19 disease severity and the development of neutralizing antibody responses in patients...

...recent paper to see the Gal4/UAS system in action (5). I use the Gal4/UAS system to knock down gene expression...2015). PubMed PMID: 21356876. 5. J. Chow, Z. Marka, I. Bartos, S. Marka, J. C. Kagan, Environmental Stress...cell fates and generating dominant phenotypes., Development 118, 401–15 (1993). PubMed PMID: 8223268. 2. ...

...your gene of interest Triton X-100 Benzonase/DNAse I (Millipore 71205-3) 40% Polyethylene Glycol 8000 (...am): Transfect cells Day 7 (am): Harvest cells Equipment Class II, Type A2 Biological Safety Cabinet 0.5...

...with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by geneticists...transformed bacteria, but you might be wondering, “How do I know which of my flies have the desired integration...as basic as survival. For those interested in development, organs of interest, such as the intestines, ...

...that will be treated in this series of posts are (i) brightness, (ii) photostability and (iii) aggregation...the Addgene Blog Which Fluorescent Protein Should I Use? Choosing Your Fluorescent Proteins for Multicolor...check all possible combinations of biology and equipment. Because the performance of a fluorescent protein...

...And while I have it on good authority that a complete Western blot can be run in a single day, I really ... while providing needed flexibility in assay development. Researchers who understand how and why to use...

...pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS & pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS. Fluorescent proteins (FP...specific cell types are missing in roots. The development of transgenic hairy roots takes at least six ...scientists do transient leaf transformations The development of transgenic plants is a common problem in non-model...might provide some useful guidelines for the development of transient transformation systems for other...

...species, with components quickly losing activity. I set out to collect the features that would define ...effectors from nanobodies to fluorescent proteins. So, I hope you will take full advantage and have lots of...is made possible through the utilization and development of genetically engineered components, like protein...

...Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Molecular Cell, 74(5): 936-950.e5 (2019... on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? The PAM requirement...can read about Cas7-11 in our blog post on its development and use. Fast facts – PAM requirement: protospacer...

...find out how you can get one. As a starting point, I would recommend conferences like Lab Robotic Interest...electronic but it is one of the most helpful machines I have used in the lab. It’s especially useful if you...DNA as opposed to in live bacteria. For these shipments, we’ve needed to find a way to purify DNA from...