We narrowed to 399 results for: promoter

Tips for naming your plasmid in a way that is clear and informative to you and your colleagues.

Acoustic reporter genes (ARGs) are activated by ultrasound instead of light. Updated bacterial and mammalians ARGs can provide contrast and improved utility.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

Joanne Kamens and Tyler Ford discuss the benefits of using Reddit to communicate your science.

Discover the best situations to use Cas9 activators in your experiments with tips from their creators.

An exploration of how the Trimmer lab developed their Addgene-deposited collection of recombinant antibodies.

Ginkgo Bioworks and Addgene are synthesizing and distributing thousands of plasmids for COVID-19 research. Read to learn more about these constructs

Overview of Cre-lox recombination and how it is used to make DNA modifications in transgenic animals, embryonic stem cells, and tissue-specific cell types.

Neil McKenna and Bert O’Malley of the Nuclear Receptor Signaling Atlas (NURSA) provide an introduction to nuclear receptors and more.

This post discusses the core replication and resistance features of yeast vectors and explains how these plasmids differ from their bacterial counterparts.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

Get perspectives and possibilities for future action from scientists and other citizens who participated in the March for Science.

There are many tools available for studying the microbiome. Learn about plasmid tools for engineering gut microbes, detection, and synthetic biology.

Learn all about new optogentics tools, caffeine degradation, biosensors, and new fluorescent proteins.

Learn about the latest CRISPR plasmids deposited at Addgene including plasmids for CRISPRi in Burkholderi, DNA and RNA editing, epitope tagging, and Cas9/gRNA ribonucleoprotein delivery.

Ten noteworthy developments in open science and scientific sharing of 2013.

Use this protocol to transfect mammalian cells with your plasmid of interest.

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

Learn about a wide variety of potential solutions to reproducibility issues in the biological sciences including changes to scientific sharing, publishing practices, and experimental design.