Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 395 results for: promoter

Showing: 121 - 140 of 395 results

  1. Working with Nuclear Receptors

    Type
    Blog Post
    ... Atlas (NURSA), one of our primary goals is to promote an understanding of how these processes are regulated...upon a context defined by ligand, cell type, and promoter, organizing the expression of functional groups...

  2. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...efficiency; however, this region does not necessarily promote replication.  Selection markers for yeast Historically...gene expression is to use a partially defective promoter to drive expression of the selection marker. This...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...expresses multiple reprogramming factors driven by one promoter. While lentivirus has similar rates of reprogramming...control. Additionally, Sendai virus doesn’t rely on promoters for transcriptional regulation, so it requires... mini-plasmids. They contain only a eukaryotic promoter and the cDNA(s) to be expressed and they don’t...phosphatase, expression of embryonic proteins, and promoter demethylation and expression of endogenous pluripotency...

  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...either the Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et al., ...known as SpyLigase and is a protein domain that promotes the formation of an isopeptide bond between 2 ...insert fragments of DNA containing basic parts (promoters, UTRs, coding sequences, terminators, etc) into... creating a single transcriptional unit (Ex: a promoter, 5’UTR, coding region, and terminator). Next, ...individual types of pre-cloned insert modules (plant promoter, N-terminal tag, coding sequence of the gene of...

  5. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ... Prior to engineering a transgenic strain, the promoter being used should be tested in cell lines to determine...with expression controlled by a tissue-specific promoter). And, Cre may also be either constitutively expressed...

  6. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...vector containing homologous ends to the breakpoint promotes homologous recombination of the targeting vector... process. The addition of small molecules that promote homologous recombination or the use of long single-stranded...

  7. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...that flank the gene cassette, which contains a promoter, the gene of interest, and a polyA signal. Created...sequences are from AAV2 because they demonstrate high promoter activity (Earley et al. 2020), but non-AAV2 ITRs...

  8. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...neuron-specific expression (hSyn promoter) and pan-mammalian cell expression (CAG promoter). An improved version ...

  10. The Twelve Days of CRISPR

    Type
    Blog Post
    ...CRISPR, Addgene gave to me: a method to capture promoter-enhancer 3D chromatin structures using CRISPR....

  13. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...components, such as your CRISPR enzyme or Pol 2 promoter. The options available in each class, or module...out reporters, Cas proteins, guide cassettes, promoters, N’ and/or C’ terminus, and selection markers....

  14. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...recombinase. If GFP is under control of an endogenous promoter, you can use expression GFP to track cells participating... physiopathological events to which the chosen promoter responds. You can also use this method to tag ...

  17. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...allows for customization with strain-specific promoters, antibiotic resistance selection markers, gRNAs...nature allows for the customization of guides, promoters, selection markers, and different versions of ...

  20. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...to measure time. These plasmids with different promoters driving expression of Cas9 were used in this study...ready-to-use AAV vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with...
Showing: 121 - 140 of 395 results