...we've already taken them for a test drive! We validated viral packaging and strong expression after inserting...characterization at IPI as well as community-led evaluation, and the results are available to view from the... of GABAergic interneurons — those marked by parvalbumin (PV), somatostatin (SST), vasoactive intestinal...neurons. The authors characterized these tools and validated their specificity in mice, non-human primates,...under the control of the BiPVe3 enhancer and parvalbumin (PV) immunostaining (green), in the primary somatosensory...

...virus, a successful AAV experiment starts with validation and optimization. Although there are different...find it is usually faster and easier to do the validation first.  AAV-mediated gene expression can differ...inducing drug, so be sure to test for this when validating your viral vector. Titer Once you have selected...measurements. The number itself is determined through validation and optimization of your expression system for...don’t. The key to good AAV selection …is truly validation and optimization! Understanding serotypes, promoters...

...mycoplasma removal from the infected culture. MRAs are non-toxic, effective in the removal of most of ...Started with Tissue Culture below! Mycoplasma removal after detection So you found mycoplasma contamination...contamination to other healthy cell lines. Mycoplasma Removal Agents (MRA) are derivatives of the quinolone family...mycoplasma. These methods cannot guarantee complete removal of mycoplasma but, can get rid of most of mycoplasma...

...of DNA transposon transposition. Modified from Sandoval-Villegas et al., 2021. To mobilize a transposon...inactivated Tc1/mariner transposons found in fish (Sandoval-Villegas et al., 2021). Sleeping Beauty’s preferred...or negative selected for during the experiment (Sandoval-Villegas et al., 2021). Target site preference... including zebrafish, mice, rats, and rabbits (Sandoval-Villegas et al., 2021).  Gene transfer Transposons...topicpage/transposons-the-jumping-genes-518/   Sandoval-Villegas N, Nurieva W, Amberger M, Ivics Z (2021...

...Preserving scientific assets: We hold a repository of valuable research materials that might otherwise be lost...and verifying them independently — particularly valuable when budgets are constrained. This collaborative...limited resources. These resources become even more valuable as labs look to maximize productivity and efficiency...impacting your work and what support would be most valuable from Addgene during this time. Your input will...

...University Cancer is one of the greatest examples of survival of the fittest. Cancer cells find a way to grow...from Madame Curie), chemotherapy, and surgical removal of the tumor. However, these conventional therapies...the immune system from fighting cancer. Surgical removal causes increases in tumor cytokines like TGFβ and...immunotherapy target using diagnostic tests and biomarker validation to identify populations that are the most likely... fosters immune privilege and predicts reduced survival." Nature medicine 10.9 (2004): 942-949. PubMed...

...important, targeting all genes doesn’t necessarily add value if you are addressing a well-defined, hypothesis...druggable targets, preferentially those for which validated inhibitors or even approved drugs already exist...smaller, secondary, CRISPR screen could be used to validate the list of candidate genes. Green Listed, as ...and then use Green Listed to design a smaller validation screen using selected gRNAs extracted from the...an easily measureable cellular phenotype (e.g. survival of a cancer cell as a cytotoxic drug is added)...

...Data Hub, an open-access repository for antibody validation data. We’ll continue growing our educational ...seizing new opportunities to create even greater value for the scientific community. As the executive director...director of Addgene, it is my privilege to uphold the values that shape Addgene’s mission and work. In 2024,...throughout 2024 on Addgene’s work, mission, and values. I welcome feedback and commentary from our diverse...

... to be able to build a highly multivalent assembly connected by covalent bonds, so there were no weak ... wanted to be able to build assemblies through covalent bonds so that the assemblies wouldn’t fall apart...2014 Mar 17. PubMed) Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial...

...buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the activity of ...you can do with these samples. This is because divalent cations are essential cofactors for many tools...downstream application involving enzymatic reactions, a valid alternative can be Tris buffer (with no EDTA) or...elute the DNA. References Murakami M (2013) Evaluation of DNA Plasmid Storage Conditions. The Open Biotechnology...

...for neuroscience research and is an expert at validating these tools. While the focus of the NABOR platform...concept of our AAV Data Hub) to share further validation data with you. “Affinity reagents are among ...an open-access, molecularly defined, and well validated collection. I’m thrilled to be working with Addgene...518027a Bradbury AM, Plückthun A (2015) Antibodies: validate recombinants once. Nature 520:295–295 . https:...

...and identifying needed skills First, evaluate your skills, values, and interests. Are you particularly...Seat” at conferences and seminars, as this is a valuable time to network Attend informational interviews...further information about shaping your career to your values, interests, and skills? Curious to know where graduate...

...not as comprehensive as this one, so it is an invaluable tool to be used in this capacity. Similar to ...molecular level. Atlas resource The TF map is a valuable resource for anyone interested in cell differentiation...differentiation programs. The Zhang lab validated their Atlas’s TF fate map by selecting a panel of candidate...network that initiates biological reprogramming is a valuable reference if you are looking to understand a pathway...

...recent studies looked at how people perceive and evaluate peer-reviewed sources of information if those ...with made up illnesses. They were then asked to evaluate the quality of the study those abstracts represented... The point of this study was to see if their evaluations of the abstracts were driven by the quality of...were engaging in a behaviour called the “biased evaluation of evidence,” in which people (conscious or unconsciously...

...only the elements needed for function. The lab validated this vector, called pBAM1, by conducting random...cell autonomy of gene function in development. To validate the use of the CRUSH vector in transgenic mice...30(11):1143-8.  A new tool for CRISPR gRNA validation Selecting a gRNA sequence that effectively...created a GFP reporter plasmid for scientists to validate the efficacy of their gRNAs. The first step is...integrated and a removable integrase cassette. They validated this methodology in two differents types of bacteria...and easy to do. Clonetegration could become a “valuable technique facilitating genetic engineering with...

...easy - I had to reinvent myself and redefine the value I could bring to potential employers. Over time,...communication style of a new, junior team member who had values, working styles, and skills that were quite different...and project management. Mentorship is another invaluable resource: connecting with mentors who have made...technical work. On the other hand, it’s perfectly valid to decide that managing isn’t for you at this point...

...for. After all, the only value your work will have in the world is the value that you can succeed in communicating...their work. While it's not always recognized and valued for its immense importance, it may well be what...them what they learned from your presentation and evaluate whether or not you’ve succeeded in communicating...

...off, F(ab’)2 fragments are bivalent, so may have higher avidity than monovalent Fab’ or Fab fragments. Higher... region and a variable region. The chains are covalently linked to one another by disulfide bonds between...antigens than a Fab or Fab’ fragment. But this bivalency may cause problems for blocking purposes, since...

...CcdB-resistant strain is ccdB Survival™ from InvitrogenTM. Using either DB3.1 or ccdB SurvivalTM, one can efficiently...same function, there is some evidence that ccdB Survival™ can be more difficult to transform, depending...would also like to note that while DB3.1 and ccdB Survival strains have been developed specifically with ...

...test them all. This is where PRIDICT comes in! Evaluate prime editing efficiencies in high-throughput ...from high-throughput experiments, where we can evaluate tens of thousands of designs in parallel. To do...coupled to their target allowing us to edit and evaluate all of the pegRNAs in one experiment (Figure 1...self-targeting screens such as PRIDICT/PRIDICT2.0 provide valuable insights into pegRNA editing efficiencies for ...