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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 130 results for: yeast expression

Showing: 121 - 130 of 130 results

  1. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...j.bbrc.2013.08.013. Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...

  2. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... CRISPR Mammalian Expression CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...plasmids have been designed for use in mammalian expression systems. Cut Fully functional CRISPR/Cas enzymes... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your... is tagged with a biotin acceptor site and co-expressed with BirA biotin ligase. The locus is isolated...

  3. CRISPR Plasmids - Plants

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...CRISPR expression plasmids that have been designed for use in plants. CRISPR... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...

  4. TALEN Plasmids and Kits

    Type
    Collection
    ... that can be used to custom repress gene expression in yeast. TALORs consist of the DNA-binding domain...-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built...Golden Gate cloning method. Optimized for human expression. Feng Zhang LIC TAL Effector Assembly Kit Assembly...than the parental vectors. pC-GoldyTALEN directs expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN...β-globin UTRs are included in the vector to enhance expression of the message. Both vectors utilize homodimeric...positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting in shorter N- and C-terminal...constructs were designed for optimal mammalian expression of Voytas Golden Gate-assembled TALEN, both in...

  5. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...has been adapted to allow tagging of proteins expressed from their natural chromosomal context. These ....1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged Cas9 AND a gRNA targeting the AAVS1... and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh donor plasmid... the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt...Drosophila cells that uses PCR to generate both an expression cassette for the Cas9-programming sgRNA and HR...

  6. Genetic Code Expansion

    Type
    Collection
    ...ttRNA pair (knocked out trpS and trpT genes). Expresses yeast TrpRS/tRNA pair instead. For directed evolution...plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate aminoacyl-tRNA-synthetase...codon is often used. The gene of interest can be expressed from a plasmid containing a UAG codon at the place...with a different amino acid. They are typically expressed from a single plasmid, with multiple copies of...make sure that these conditions are true. Always express first with a control reporter gene – GFP for E....mCherry-GFP for mammalian cells. You should also express the protein with and without the ncAA in the media...

  7. Molecular Biology Reference

    Type
    Guide
    ...information. Expression Plasmids - Used for gene expression (for the purposes of gene study). Expression vectors... produced. Expression vectors can drive expression in various cell types (mammalian, yeast, bacterial,... the level of insert expression (i.e., a strong promoter directs high expression, whereas weaker promoters...reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA... control of a bacterial promoter, and is thus expressed in the bacteria by bacterial transcriptional machinery...restriction enzymes digestion and ligation. In expression plasmids, the MCS is often located downstream...that when a gene is inserted within the MCS, its expression will be driven by the promoter. As a general ...

  8. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate CRISPR ...Multiple gRNAs targeting multiple loci can be easily expressed in the same cell or organism. The ability to precisely...Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ) Zebrafish Xenopus...

  9. CRISPR Guide

    Type
    Guide
    ...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...Knockout libraries often target 5′ constitutively expressed exons, while activation and repression libraries...libraries are also available, such as those for yeast or drosophila . Although CRISPR has been less widely...with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After applying your screening...

  10. CRISPR Guide

    Type
    Collection
    ...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...Knockout libraries often target 5′ constitutively expressed exons, while activation and repression libraries...libraries are also available, such as those for yeast or drosophila . Although CRISPR has been less widely...with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After applying your screening...
Showing: 121 - 130 of 130 results