We narrowed to 124 results for: yeast expression

1. CRISPR History and Development for Genome Engineering

   Type

   Collection

   ...known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate CRISPR ...Multiple gRNAs targeting multiple loci can be easily expressed in the same cell or organism. The ability to precisely...Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ) Zebrafish Xenopus...

2. Molecular Biology Reference

   Type

   Guide

   ...information. Expression Plasmids - Used for gene expression (for the purposes of gene study). Expression vectors... produced. Expression vectors can drive expression in various cell types (mammalian, yeast, bacterial,... the level of insert expression (i.e., a strong promoter directs high expression, whereas weaker promoters...reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA... control of a bacterial promoter, and is thus expressed in the bacteria by bacterial transcriptional machinery...restriction enzymes digestion and ligation. In expression plasmids, the MCS is often located downstream...that when a gene is inserted within the MCS, its expression will be driven by the promoter. As a general ...

3. CRISPR Guide

   Type

   Guide

   ...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...Knockout libraries often target 5′ constitutively expressed exons, while activation and repression libraries...libraries are also available, such as those for yeast or drosophila . Although CRISPR has been less widely...with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After applying your screening...

4. CRISPR Guide

   Type

   Collection

   ...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...Knockout libraries often target 5′ constitutively expressed exons, while activation and repression libraries...libraries are also available, such as those for yeast or drosophila . Although CRISPR has been less widely...with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After applying your screening...