We narrowed to 1,010 results for: RING;
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TypeBlog Post...purification preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce...events that occur on a short timescale, such as during drug action or cell signaling. Limitations of RaPID...
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dTAG - You're it!
TypeBlog Post...as cereblon (CRBN) or von Hippel-Lindau (VHL) to bring the endogenous degradation machinery into close ...lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal fusions and... -
Visualizing Protein Turnover In Situ
TypeBlog Post...influence behavior. A conference and a breakthrough! During my graduate studies I attended a scientific symposium...inspiration to resolve the technical hurtle of monitoring protein turnover of specific proteins in situ... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog Post...you on LinkedIn, you will lose opportunities and hiring managers will think it is odd. For those planning...any of these people, including and especially the hiring manager. This is considered intrusive and it’s ... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...rather blunt instrument for controlling CRISPR. To bring greater control to Acr activity as a biological ...benthamiana or Arabidopsis thaliana (Xing et al., 2016)). During Agrobacterium-mediated DNA transfer, each GOI is... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post... a musician, then metastasize is touring. But you know what a touring musician needs? A backstage team... -
Plasmids 101: Cre-lox
TypeBlog Post...enough that there is virtually no chance of it occurring randomly. Therefore, inserting loxP sites at deliberate...are active only in certain cell or tissue types, during certain stages of development, or by making the... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...informing potential for success in a patient-mutation bearing knock-in mouse model for CTD. Congratulations the...questions,” Hébert says. In the spirit of plasmid sharing and open science, Hébert plans on building upon... -
Antibodies 101: Affinity Reagents
TypeBlog Post...similar to a Fab fragment but created through engineering instead of cleavage. They are comprised of the...stable up to 80°C and can refold correctly after denaturing. Designed ankyrin repeat proteins, or DARPins... -
Antibodies 101: Conjugation
TypeBlog Post...spectral properties of the fluorophores you are considering to make sure that they’ll be compatible with ...target. I love FPbase’s Spectra Viewer tool for comparing different fluorophores. We also touched on fluorophores...