We narrowed to 1,010 results for: RING;
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TypeBlog Post...and Ghyselinck NB. A directional strategy for monitoring Cre-mediated recombination at the cellular level...Addgene Blog Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector...
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Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...change (facilitated by the flexible linker) that brings the fluorescent units of the FRET pair together... and diabetes mortality determined by nutrition during parents' and grandparents' slow growth period."... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...mouse knockout lines should increase. New genome engineering tools also offer hope for the creation of new...knockout animals or cell lines, please consider sharing them by depositing with Addgene! Figures were ... -
Hot Plasmids: Winter 2025
TypeBlog Post...to dicot species. In addition, they generated offspring tomato plants that inherited the genomic edit ...If they do (or if they don't!), please consider sharing your experience with other researchers by contributing... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...the Challenges of Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on Addgene.org...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...downstream flanking sequences to their input string (Figure 2). This string can then be used as input for the prediction... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post... use in the protocol, which cover 3 naturally-occurring nanobody structures (concave, loop, and convex...translated protein (sybody), ribosome, and mRNA. During the screening process, displayed sybodies that ... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...expression baseline. Copy number is then determined by comparing results to a standard curve. For CNV assays, it...gives an absolute value of DNA concentration. By comparing the number of positive droplets for the gene of... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...supernumerary preimplantation embryos that are produced during in vitro fertilization treatments and donated afterwards...produced Ethical concern High. Embryos are destroyed during ESC isolation. Low Chances of post-transplantation... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...their mental model of the protein. At some point during this interrogation process, they will almost invariably...recognize a PAM sequence, allow the complementary base pairing between the guide RNA and the target DNA strand...