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We narrowed to 960 results for: comp

Showing: 751 - 760 of 960 results
  1. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post
    ... creates a non-functional 𝛽-galactosidase. Complementing this mutation by expressing this deleted portion...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-...
  2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post
    ...increase in the number of endogenous indel edits when compared to unsorted cells (Wen et al., 2018). Thus SRIRACCHA...expression. As a proof of concept the Denham lab compared editing at three different genomic sites in the...
  3. Plasmids 101: Methylation and Restriction Enzymes

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    Blog Post
    ...protect themselves from foreign DNA. The other component of these systems, methyltransferases, methylate...add methyl groups to specific DNA sequences and companion endonucleases that recognizes and cleave the same...
  4. Top Requested Lentivirus and AAV of 2016

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    Blog Post
    ...lentiviruses expressing various gRNAs. It’s better to compare the phenotypic effects of expressing various gRNAs...expression between experiments and over time (as compared to separate transductions with a single-vector...
  5. Viral Vectors 101: Viral Applications

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    Blog Post
    ...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction... virus to which immunity is desired. Instead, components of the virus are now delivered in other forms...
  6. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post
    ...binding of a new inactive heterotrimeric G protein complex and subsequently another round of signaling.    ...gamma subunits of the heterotrimeric G protein complex that can be used to detect G protein activation...
  7. Antibodies 101: Secondary Antibodies

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    Blog Post
    ...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies... (And while I have it on good authority that a complete Western blot can be run in a single day, I really...
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